LOCUS       sequence-200785-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR42; Opto-GPR42; B12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR42
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtctttg tgggcaagct gcggtgccgc
      301 ccggtggccc tcaactacat cctgctcaac ctggccgtgg ccgacctctt catggtgttc
      361 gggggcttca ccaccaccct ctacacctct ctgcacgggt acttcgtctt tgggcccacg
      421 ggctgcaacc tggagggctt ctttgccacc ttgggcggtg aaattgcact gtggtccttg
      481 gtggtcctgg ccatcgagcg gtacgtggtg gtggcccacc cactgtggta caagacccgg
      541 ccgaggctgg ggcaggccat catgggcgtc gccttcacct gggtcatggc tctggcctgt
      601 gccgcgcccc ccctcgtcgg ctggtccagg tacatcccgg agggcatgca gtgctcgtgc
      661 gggattgact actacacgcc ccacgaggaa accaacaatg agtcgttcgt catctacatg
      721 ttcgtggtcc acttcatcat ccccctgatt gtcatattct tctgctacgg acgcctggtg
      781 tggatcctcg gcagaggggg cagccaccgc cggcagagga gggtggcgcg catggtgatc
      841 atcatggtca tcgctttcct aatctgctgg ctgccctacg ctggggtggc gttctacatc
      901 ttcacccatc agggctctga ctttggcccc atcttcatga ccatcccggc tttctttgcc
      961 aagacttctg ccgtctacaa ccccgtcatc tacatcatga tgaacaagca gttccgcgcc
     1021 gactttcatg agctgctgag gaggttgtgt gggctctggg gccagtggca gcaggagagc
     1081 agcatggagc tgaaggagca gaagggaggg gaggagcaga gagcggaccg accagctgaa
     1141 agaaagacca gtgaacactc acagggctgt ggaactggtg gccaggtggc ctgtgctgaa
     1201 aacacagaaa ccagccaagt ggcgcctgcc taa
//
