LOCUS       sequence-200786-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR45; Opto-GPR45; C1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR45
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtgt accagaggcc ggctatgcgc
      301 tcggccctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg gtccagcgcc aggacaagct gaacccgcgc
      541 agggccatca tgggcgtcgc cttcacctgg gtcatggctc tggcctgtgc cgcgcccccc
      601 ctcgtcggct ggtccaggta catcccggag ggcatgcagt gctcgtgcgg gattgactac
      661 tacacgcccc acgaggaaac caacaatgag tcgttcgtca tctacatgtt cgtggtccac
      721 ttcatcatcc ccctgattgt catattcttc tgctacggat gcatcctcaa cacggtccgc
      781 aagaacgccg tgcgcgtgca caaccagtcg gacagcctgg acctgcggca gctcaccagg
      841 gcgggcctgc ggcgcctgca gcggcagcaa caggtcagcg tggacttgag cttcaagacc
      901 aaggccttcc gcatggtgat catcatggtc atcgctttcc taatctgctg gctgccctac
      961 gctggggtgg cgttctacat cttcacccat cagggctctg actttggccc catcttcatg
     1021 accatcccgg ctttctttgc caagacttct gccgtctaca accccgtcat ctacatcatg
     1081 atgaacaagc agttccgcga ggcctgcata gagttgctgc cccagacctt ccaaatcctc
     1141 cccaaagtgc ctgagcggat ccgaaggaga atccagccaa gcacagtcta cgtgtgcaat
     1201 gaaaaccagt ctgcggttac agaaaccagc caagtggcgc ctgcctaa
//
