LOCUS       sequence-200787-        2013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR50; Opto-GPR50; C2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR50
FEATURES             Location/Qualifiers
     source          1..2013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtga cgaagaacaa gaagctccgg
      301 aattctctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg tgccacagcc tccagtacga acggatcttc
      541 agtgtgcgca atgccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaag gatctggacc
      781 aaagtgctgg cggcccgtga ccctgcaggg cagaatcctg acaaccaact tgctgaggtt
      841 cgcaattttc gcatggtgat catcatggtc atcgctttcc taatctgctg gctgccctac
      901 gctggggtgg cgttctacat cttcacccat cagggctctg actttggccc catcttcatg
      961 accatcccgg ctttctttgc caagacttct gccgtctaca accccgtcat ctacatcatg
     1021 atgaacaagc agttccgcag agaatactgg accatcttcc atgctatgcg gcaccctatc
     1081 atattcttct ctggcctcat cagtgatatt cgtgagatgc aggaggcccg taccctggcc
     1141 cgcgcccgtg cccatgctcg cgaccaagct cgtgaacaag accgtgccca tgcctgtcct
     1201 gctgtggagg aaaccccgat gaatgtccgg aatgttccat tacctggtga tgctgcagct
     1261 ggccaccccg accgtgcctc tggccaccct aagccccatt ccagatcctc ctctgcctat
     1321 cgcaaatctg cctctaccca ccacaagtct gtctttagcc actccaaggc tgcctctggt
     1381 cacctcaagc ctgtctctgg ccactccaag cctgcctctg gtcaccccaa gtctgccact
     1441 gtctacccta agcctgcctc tgtccatttc aaggctgact ctgtccattt caagggtgac
     1501 tctgtccatt tcaagcctga ctctgttcat ttcaagcctg cttccagcaa ccccaagccc
     1561 atcactggcc accatgtctc tgctggcagc cactccaagt ctgccttcag tgctgccacc
     1621 agccacccta aacccaccac tggccacatc aagccagcta ccagccatgc agagcccacc
     1681 actgctgact atcccaagcc tgccactacc agccacccta agcccactgc tgctgacaac
     1741 cctgagctct ctgcctccca ttgccccgag atccctgcca ttgcccaccc tgtgtctgac
     1801 gacagtgacc tccctgagtc ggcctctagc cctgccgctg ggcccaccaa gcctgctgcc
     1861 agccagctgg agtctgacac catcgctgac cttcctgacc ctactgtagt cactaccagt
     1921 accaatgatt accatgatgt cgtggttatt gatgttgaag atgatcctga tgaaatggct
     1981 gtgacagaaa ccagccaagt ggcgcctgcc taa
//
