LOCUS       sequence-200788-        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR52; Opto-GPR52; C3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR52
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtctttc attgtgctcc actgttacat
      301 cattatactc tcaactacat cctgctcaac ctggccgtgg ccgacctctt catggtgttc
      361 gggggcttca ccaccaccct ctacacctct ctgcacgggt acttcgtctt tgggcccacg
      421 ggctgcaacc tggagggctt ctttgccacc ttgggcggtg aaattgcact gtggtccttg
      481 gtggtcctgg ccatcgagcg gtacgtggtg gtgaccaagc ctctttccta caatcaactg
      541 gtcacccctt gtcgcgccat catgggcgtc gccttcacct gggtcatggc tctggcctgt
      601 gccgcgcccc ccctcgtcgg ctggtccagg tacatcccgg agggcatgca gtgctcgtgc
      661 gggattgact actacacgcc ccacgaggaa accaacaatg agtcgttcgt catctacatg
      721 ttcgtggtcc acttcatcat ccccctgatt gtcatattct tctgctacgg acacattttc
      781 aaaatttgcc gtcagcacac caaagagata aatgaccgaa gggcccgatt ccctagtcat
      841 gaggtagatt cttccagaga gactggacac agccctgacc gtcgctacgc ccgcatggtg
      901 atcatcatgg tcatcgcttt cctaatctgc tggctgccct acgctggggt ggcgttctac
      961 atcttcaccc atcagggctc tgactttggc cccatcttca tgaccatccc ggctttcttt
     1021 gccaagactt ctgccgtcta caaccccgtc atctacatca tgatgaacaa gcagttccgc
     1081 ctaggcctcc gaaggctgtc tgagacaatg tgcacatcct gtatgtgtgt gaaggatcag
     1141 gaagcacaag aacccaaacc taggaaacgg gctaattctt gctccattac agaaaccagc
     1201 caagtggcgc ctgcctaa
//
