LOCUS       sequence-200789-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR55; Opto-GPR55; C4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR55
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcttta gcaccttcct taagaacagg
      301 tggcccgatt atgctgccct caactacatc ctgctcaacc tggccgtggc cgacctcttc
      361 atggtgttcg ggggcttcac caccaccctc tacacctctc tgcacgggta cttcgtcttt
      421 gggcccacgg gctgcaacct ggagggcttc tttgccacct tgggcggtga aattgcactg
      481 tggtccttgg tggtcctggc catcgagcgg tacgtggtgg tgcgttaccc gctactggtg
      541 agccacctcc ggtcccccag gaaggccatc atgggcgtcg ccttcacctg ggtcatggct
      601 ctggcctgtg ccgcgccccc cctcgtcggc tggtccaggt acatcccgga gggcatgcag
      661 tgctcgtgcg ggattgacta ctacacgccc cacgaggaaa ccaacaatga gtcgttcgtc
      721 atctacatgt tcgtggtcca cttcatcatc cccctgattg tcatattctt ctgctacgga
      781 aggagcatcc acatcctgct gggccgccga gatcacaccc aggactgggt gcagcagaaa
      841 gcctgcatcc gcatggtgat catcatggtc atcgctttcc taatctgctg gctgccctac
      901 gctggggtgg cgttctacat cttcacccat cagggctctg actttggccc catcttcatg
      961 accatcccgg ctttctttgc caagacttct gccgtctaca accccgtcat ctacatcatg
     1021 atgaacaagc agttccgcat gaacatcagg gcccaccggc cttccagggt ccagctggtc
     1081 ctgcaggaca ccacgatctc ccggggcaca gaaaccagcc aagtggcgcc tgcctaa
//
