LOCUS       sequence-200790-        1461 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR61; Opto-GPR61; C5).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR61
FEATURES             Location/Qualifiers
     source          1..1461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcatcg ccaagacgcc tgccctccga
      301 aaactcaact acatcctgct caacctggcc gtggccgacc tcttcatggt gttcgggggc
      361 ttcaccacca ccctctacac ctctctgcac gggtacttcg tctttgggcc cacgggctgc
      421 aacctggagg gcttctttgc caccttgggc ggtgaaattg cactgtggtc cttggtggtc
      481 ctggccatcg agcggtacgt ggtggtggtc caccccatgc gctacgaggt gcgcatgacg
      541 ctggggctgg ccatcatggg cgtcgccttc acctgggtca tggctctggc ctgtgccgcg
      601 ccccccctcg tcggctggtc caggtacatc ccggagggca tgcagtgctc gtgcgggatt
      661 gactactaca cgccccacga ggaaaccaac aatgagtcgt tcgtcatcta catgttcgtg
      721 gtccacttca tcatccccct gattgtcata ttcttctgct acggaagcat gttccgagtg
      781 gcccgcgtgg ctgccatgca gcacgggccg ctgcccacgt ggatggagac accccggcaa
      841 cgctccgaat ctctcagcag ccgctccacg atggtcacca gctcgggggc cccccagacc
      901 accccacacc ggacgtttgg gggagggaaa gcagcacgca tggtgatcat catggtcatc
      961 gctttcctaa tctgctggct gccctacgct ggggtggcgt tctacatctt cacccatcag
     1021 ggctctgact ttggccccat cttcatgacc atcccggctt tctttgccaa gacttctgcc
     1081 gtctacaacc ccgtcatcta catcatgatg aacaagcagt tccgcgggga gttgagcaag
     1141 cagtttgtct gcttcttcaa gccagctcca gaggaggagc tgaggctgcc tagccgggag
     1201 ggctccattg aggagaactt cctgcagttc cttcagggga ctggctgtcc ttctgagtcc
     1261 tgggtttccc gacccctacc cagccccaag caggagccac ctgctgttga ctttcgaatc
     1321 ccaggccaga tagctgagga aacctctgag ttcctggagc agcaactcac cagcgacatc
     1381 atcatgtcag acagctacct ccgtcctgcc gcctcacccc ggctggagtc aacagaaacc
     1441 agccaagtgg cgcctgccta a
//
