LOCUS       sequence-200797-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR82; Opto-GPR82; C12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR82
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtctttt taacaaaaat aggtaaaaaa
      301 acatcactca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg tgctatgaga aaatatttta tggccattta
      541 ctgaaaaaat ttcgccagcc caactttgcc atcatgggcg tcgccttcac ctgggtcatg
      601 gctctggcct gtgccgcgcc ccccctcgtc ggctggtcca ggtacatccc ggagggcatg
      661 cagtgctcgt gcgggattga ctactacacg ccccacgagg aaaccaacaa tgagtcgttc
      721 gtcatctaca tgttcgtggt ccacttcatc atccccctga ttgtcatatt cttctgctac
      781 ggatcttttg taagccatct gagaaaaata agaacctgta cgtccattat ggagaaagat
      841 ttgacttaca gttctgtgcg catggtgatc atcatggtca tcgctttcct aatctgctgg
      901 ctgccctacg ctggggtggc gttctacatc ttcacccatc agggctctga ctttggcccc
      961 atcttcatga ccatcccggc tttctttgcc aagacttctg ccgtctacaa ccccgtcatc
     1021 tacatcatga tgaacaagca gttccgcaaa acactatata atctctttac aaagtctaat
     1081 tcagcacata tgcaatcata tggtacagaa accagccaag tggcgcctgc ctaa
//
