LOCUS       sequence-200804-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR120; Opto-GPR120; D7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR120
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtgg cgcgccgacg acgccgcggc
      301 gcgctcaact acatcctgct caacctggcc gtggccgacc tcttcatggt gttcgggggc
      361 ttcaccacca ccctctacac ctctctgcac gggtacttcg tctttgggcc cacgggctgc
      421 aacctggagg gcttctttgc caccttgggc ggtgaaattg cactgtggtc cttggtggtc
      481 ctggccatcg agcggtacgt ggtggtggtg cacctgcagc gcggcgtgcg gggtcctggg
      541 cggcgggcgg ccatcatggg cgtcgccttc acctgggtca tggctctggc ctgtgccgcg
      601 ccccccctcg tcggctggtc caggtacatc ccggagggca tgcagtgctc gtgcgggatt
      661 gactactaca cgccccacga ggaaaccaac aatgagtcgt tcgtcatcta catgttcgtg
      721 gtccacttca tcatccccct gattgtcata ttcttctgct acggaaaaat tttacagacc
      781 tcggaacacc tcctggatgc aagagctgtc gtgactcaca gtgagatcac aaaggcatca
      841 aggaagaggc tcacggtaag cctggcctac tcggagagcc accagatccg cgtgtcccag
      901 caggacttcc ggctgttccg catggtgatc atcatggtca tcgctttcct aatctgctgg
      961 ctgccctacg ctggggtggc gttctacatc ttcacccatc agggctctga ctttggcccc
     1021 atcttcatga ccatcccggc tttctttgcc aagacttctg ccgtctacaa ccccgtcatc
     1081 tacatcatga tgaacaagca gttccgctgg aagaaaattt tttgctgctt ctggttccca
     1141 gaaaagggag ccattttaac agacacatct gtcaaaagaa atgacttgtc gattatttct
     1201 ggcacagaaa ccagccaagt ggcgcctgcc taa
//
