LOCUS       sequence-200806-        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR135; Opto-GPR135; D9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR135
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcattg tgaagcaccg gcagctccgc
      301 accgtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg gtgcgcccgc cgcgggagaa gatcggccgc
      541 cgccgcgcca tcatgggcgt cgccttcacc tgggtcatgg ctctggcctg tgccgcgccc
      601 cccctcgtcg gctggtccag gtacatcccg gagggcatgc agtgctcgtg cgggattgac
      661 tactacacgc cccacgagga aaccaacaat gagtcgttcg tcatctacat gttcgtggtc
      721 cacttcatca tccccctgat tgtcatattc ttctgctacg gacacatctg caagacggtg
      781 cgcctgtcgg acgtgcgcgt gcggccggtg aacacctacg cgcgcgtgct gcgcttcttc
      841 agcgaggtgc gcacgcgcat ggtgatcatc atggtcatcg ctttcctaat ctgctggctg
      901 ccctacgctg gggtggcgtt ctacatcttc acccatcagg gctctgactt tggccccatc
      961 ttcatgacca tcccggcttt ctttgccaag acttctgccg tctacaaccc cgtcatctac
     1021 atcatgatga acaagcagtt ccgccgcaac cgcgaggagg gctaccggac taggaatgtg
     1081 gacgctttcc tgcccagcca gggcccgggt ctgcaagcca gaagccgcag tcgccttcga
     1141 aaccgctatg ccaaccggct gggggcctgc aacaggatgt cctcttccaa cccggccagc
     1201 ggagtggcag gggacgtggc catgtgggcc cgcaaaaatc cagttgtact tttctgccga
     1261 gagggaccac cagagccggt gacggcagtg accaaacagc ctaaatccga agctggggat
     1321 accagcctca cagaaaccag ccaagtggcg cctgcctaa
//
