LOCUS       sequence-200808-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR141; Opto-GPR141; D11).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR141
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcctgg tgaaaatgaa cacccggtca
      301 gtgctcaact acatcctgct caacctggcc gtggccgacc tcttcatggt gttcgggggc
      361 ttcaccacca ccctctacac ctctctgcac gggtacttcg tctttgggcc cacgggctgc
      421 aacctggagg gcttctttgc caccttgggc ggtgaaattg cactgtggtc cttggtggtc
      481 ctggccatcg agcggtacgt ggtggtgttc aagtgcaaag acaaagtgga attctacaga
      541 aaactggcca tcatgggcgt cgccttcacc tgggtcatgg ctctggcctg tgccgcgccc
      601 cccctcgtcg gctggtccag gtacatcccg gagggcatgc agtgctcgtg cgggattgac
      661 tactacacgc cccacgagga aaccaacaat gagtcgttcg tcatctacat gttcgtggtc
      721 cacttcatca tccccctgat tgtcatattc ttctgctacg gaatcattat gttgatggtg
      781 cagaagctac gccactcttt actatcccac caggagttct gggcacagcg catggtgatc
      841 atcatggtca tcgctttcct aatctgctgg ctgccctacg ctggggtggc gttctacatc
      901 ttcacccatc agggctctga ctttggcccc atcttcatga ccatcccggc tttctttgcc
      961 aagacttctg ccgtctacaa ccccgtcatc tacatcatga tgaacaagca gttccgccaa
     1021 aagataattg gcttatggaa ttgtgttttg tgccgtacag aaaccagcca agtggcgcct
     1081 gcctaa
//
