LOCUS       sequence-200812-        2214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR149; Opto-GPR149; E3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR149
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcctgc tgaaaatgca gaacagaact
      301 gttctcaact acatcctgct caacctggcc gtggccgacc tcttcatggt gttcgggggc
      361 ttcaccacca ccctctacac ctctctgcac gggtacttcg tctttgggcc cacgggctgc
      421 aacctggagg gcttctttgc caccttgggc ggtgaaattg cactgtggtc cttggtggtc
      481 ctggccatcg agcggtacgt ggtggtgcac agaggtgtgg ggagccagac agcctccaga
      541 agatcgggcg ccatcatggg cgtcgccttc acctgggtca tggctctggc ctgtgccgcg
      601 ccccccctcg tcggctggtc caggtacatc ccggagggca tgcagtgctc gtgcgggatt
      661 gactactaca cgccccacga ggaaaccaac aatgagtcgt tcgtcatcta catgttcgtg
      721 gtccacttca tcatccccct gattgtcata ttcttctgct acggagaaat ttcccgtgga
      781 gcttcaattc ctgggacccc tcctactgcg gggagagtgg tttccctgtc cccagaggat
      841 gctccaggcc cgagtctgcg gcgctctggg ggatgctctc cgagctccga caccgtgttc
      901 ggaccgggtg cgcccgctgc cgctggggct gaagcctgca ggcgtgagaa ccgggggact
      961 ctctatggca ccaggagctt caccgtgagc gtagcgcaga agcgcatggt gatcatcatg
     1021 gtcatcgctt tcctaatctg ctggctgccc tacgctgggg tggcgttcta catcttcacc
     1081 catcagggct ctgactttgg ccccatcttc atgaccatcc cggctttctt tgccaagact
     1141 tctgccgtct acaaccccgt catctacatc atgatgaaca agcagttccg cctatcattc
     1201 caaaaaagtt atgggattta taaaatagca catgaagatt actatgatga tgatgaaaat
     1261 tccatattct atcacaacct gatgaactct gagtgtgaaa ctacaaaaga ccctcagaga
     1321 gacaaccgta acatcttcaa tgctataaaa gtagaaatca gcaccacgcc ctctctggac
     1381 agctccacac aaagaggcat caacaaatgc acaaatactg atattacaga agctaaacag
     1441 gattccaaca acaaaaagga tgcgttttct gacaaaacag gaggtgatat taactatgaa
     1501 gaaactacct tttctgaagg gccagaaaga aggctgtctc atgaagagag tcagaaacca
     1561 gatctttcag actgggagtg gtgtaggagt aaatcagaaa gaacccctcg tcagcgttcc
     1621 ggttatgccc ttgccattcc cttgtgtgca ttccagggga ctgtgtctct ccatgcacct
     1681 acagggaaaa ccctatctct ttctacctat gaggtaagcg cagaagggca aaaaataact
     1741 ccagcctcta agaaaataga agtctatcga tccaaaagtg ttggccatga accaaactca
     1801 gaagattctt catccacgtt tgtggacacc agtgtgaaaa tacacttgga ggttcttgaa
     1861 atttgtgata atgaagaggc cttggacact gtgtcaatca ttagtaacat cagtcagtcc
     1921 tccacacaag tcagatctcc atccctacgt tactccagga aagaaaacag atttgtttca
     1981 tgtgacctag gggaaacagc ctcatactcc ctctttttgc ccaccagtaa tcctgatggt
     2041 gatattaata tctccattcc agacacagta gaagcacaca ggcagaacag taaaaggcag
     2101 catcaagaga gggatggcta ccaggaggaa atccagttgt taaataaagc ttacagaaaa
     2161 agagaggaag aaagcaaggg tagtacagaa accagccaag tggcgcctgc ctaa
//
