LOCUS       sequence-200813-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR150; Opto-GPR150; E4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR150
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcctgt gcggcggcgg cgggccctgg
      301 gcgggcccca agcgtcgcaa gctcaactac atcctgctca acctggccgt ggccgacctc
      361 ttcatggtgt tcgggggctt caccaccacc ctctacacct ctctgcacgg gtacttcgtc
      421 tttgggccca cgggctgcaa cctggagggc ttctttgcca ccttgggcgg tgaaattgca
      481 ctgtggtcct tggtggtcct ggccatcgag cggtacgtgg tggtgcggct cccgcacggc
      541 cggccgctgc ccgccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaca cctactctcc
      781 gtctggtggc ggcaccggcc gcaggccccc gcggctgcag cgccctggtc ggcgagccca
      841 ggtcgagccc ctgcgcccag cgcgctgccc cgcgccaagg tgcagagccg catggtgatc
      901 atcatggtca tcgctttcct aatctgctgg ctgccctacg ctggggtggc gttctacatc
      961 ttcacccatc agggctctga ctttggcccc atcttcatga ccatcccggc tttctttgcc
     1021 aagacttctg ccgtctacaa ccccgtcatc tacatcatga tgaacaagca gttccgccga
     1081 cagctgcgga agcggctggg ctctctgtgc tgcgcgccgc agggaggcgc ggaggacgag
     1141 gaggggcccc ggggccacca ggcgctctac cgccaacgct ggccccaccc tcattatcac
     1201 catgctcggc gggaaccgct ggacgagggc ggcttgcgcc caccccctcc gcgccccaga
     1261 cccctgcctt gctcctgcga aagtgccttc acagaaacca gccaagtggc gcctgcctaa
//
