LOCUS       sequence-200814-        1380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR151; Opto-GPR151; E5).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR151
FEATURES             Location/Qualifiers
     source          1..1380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcctcc ttcacaatgc ttggaaagga
      301 aagccatcca tgctcaacta catcctgctc aacctggccg tggccgacct cttcatggtg
      361 ttcgggggct tcaccaccac cctctacacc tctctgcacg ggtacttcgt ctttgggccc
      421 acgggctgca acctggaggg cttctttgcc accttgggcg gtgaaattgc actgtggtcc
      481 ttggtggtcc tggccatcga gcggtacgtg gtggtgagtg acccagccaa gcaagtgagt
      541 atccacaact acgccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaag agcttatgac
      781 caatgtaaaa aacgaggaac taagactcaa aatcttagaa accagatacg ctcaaagcaa
      841 gtcacacgca tggtgatcat catggtcatc gctttcctaa tctgctggct gccctacgct
      901 ggggtggcgt tctacatctt cacccatcag ggctctgact ttggccccat cttcatgacc
      961 atcccggctt tctttgccaa gacttctgcc gtctacaacc ccgtcatcta catcatgatg
     1021 aacaagcagt tccgcgaagg cttgaaaggt gtatggaaat ggatgataac caaaaaacct
     1081 ccaactgtct cagagtctca ggaaacacca gctggcaact cagagggcct ccctgacaag
     1141 gttccatctc cagaatcccc agcatccata ccagaaaaag agaaacccag ctctccctcc
     1201 tctggcaaag ggaaaactga gaaggcagag attcccatcc ttcctgacgt agagcagttt
     1261 tggcatgaga gggacacagt cccttctgta caggacaatg accctatccc ctgggaacat
     1321 gaagatcaag agacagggga aggtgttaaa acagaaacca gccaagtggc gcctgcctaa
//
