LOCUS       sequence-200815-        1563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR152; Opto-GPR152; E6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR152
FEATURES             Location/Qualifiers
     source          1..1563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgccg gctcccaggc ccggcatgga
      301 gctggcacgc gactcaacta catcctgctc aacctggccg tggccgacct cttcatggtg
      361 ttcgggggct tcaccaccac cctctacacc tctctgcacg ggtacttcgt ctttgggccc
      421 acgggctgca acctggaggg cttctttgcc accttgggcg gtgaaattgc actgtggtcc
      481 ttggtggtcc tggccatcga gcggtacgtg gtggtgctgt gcccacactg gtaccctggg
      541 caccgcccag tccgcctggc catcatgggc gtcgccttca cctgggtcat ggctctggcc
      601 tgtgccgcgc cccccctcgt cggctggtcc aggtacatcc cggagggcat gcagtgctcg
      661 tgcgggattg actactacac gccccacgag gaaaccaaca atgagtcgtt cgtcatctac
      721 atgttcgtgg tccacttcat catccccctg attgtcatat tcttctgcta cggactcacc
      781 caggccacag cctgtcgcac ctgccaccgc caacagcagc ccgcagcctg ccggggcttc
      841 gcccgcatgg tgatcatcat ggtcatcgct ttcctaatct gctggctgcc ctacgctggg
      901 gtggcgttct acatcttcac ccatcagggc tctgactttg gccccatctt catgaccatc
      961 ccggctttct ttgccaagac ttctgccgtc tacaaccccg tcatctacat catgatgaac
     1021 aagcagttcc gcaccctgct gcgctccgtg ctctcgtcct tcgcggcagc tctctgcgag
     1081 gagcggccgg gcagcttcac gcccactgag ccacagaccc agctagattc tgagggtcca
     1141 actctgccag agccgatggc agaggcccag tcacagatgg atcctgtggc ccagcctcag
     1201 gtgaacccca cactccagcc acgatcggat cccacagcac agccacagct gaaccctacg
     1261 gcccagccac agtcggatcc cacagcccag ccacagctga acctcatggc ccagccacag
     1321 tcagattctg tggcccagcc acaggcagac actaacgtcc agacccctgc acctgctgcc
     1381 agttctgtgc ccagtccctg tgatgaagct tccccaaccc catcctcgca tcctacccca
     1441 ggggcccttg aggacccagc cacacctcct gcctctgaag gagaaagccc cagcagcacc
     1501 ccgccagagg cggccccggg cgcaggcccc acgacagaaa ccagccaagt ggcgcctgcc
     1561 taa
//
