LOCUS       sequence-200816-        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR153; Opto-GPR153; E7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR153
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgttg gcgccaagca gaagaagtgg
      301 aagcccctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg tgctggcctg tcaactaccg attgagcaat
      541 gccaagaagc aggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaac gctggccgtg
      781 caggtggggc gccaggccga ccgccgcgcc ttcaccgtgc ccaccatcgt ggtggaggac
      841 gcgcagggca agcggcgctc ctccatcgat ggctcggagc ccgccaaaac ctctctgcag
      901 cgcatggtga tcatcatggt catcgctttc ctaatctgct ggctgcccta cgctggggtg
      961 gcgttctaca tcttcaccca tcagggctct gactttggcc ccatcttcat gaccatcccg
     1021 gctttctttg ccaagacttc tgccgtctac aaccccgtca tctacatcat gatgaacaag
     1081 cagttccgcg ctgacctcaa agctgtccgg gagaagtgca tggccctcat ggccaacgac
     1141 gaggagtcag acgatgaaac cagcctggaa ggtggcatct ccccggacct ggtgttggag
     1201 cgctccctgg actatggcta tggaggtgat tttgtggccc tagataggat ggccaagtat
     1261 gagatctccg ccctggaggg gggcctgccc cagctctacc cactgcggcc cttgcaggag
     1321 gacaagatgc aatacctgca ggtcccgccc acgcggcgct tctcccacga cgatgcggac
     1381 gtgtgggccg ccgtcccgct gcccgccttc ctgccgcgct ggggctccgg cgaggacctg
     1441 gccgccctgg cgcacctggt gctgcctgcc gggcccgagc ggcgccgcgc cagcctcctg
     1501 gccttcgcgg aggacgcacc accgtcccgc gcgcgccgcc gctcggccga gagcctgctg
     1561 tcgctgcggc cctcggccct ggatagcggc ccgcggggag cccgcgactc gccccccggc
     1621 agcccgcgcc gccgccccgg gcccggcccc cgctccgcct cggcctcgct gctgcccgac
     1681 gccttcgccc tgaccgcctt cgagtgcgag ccacaggccc tgcgccgccc gcccgggccc
     1741 ttccccgctg cgcccgccgc ccccgacggc gcagatcccg gagaggcccc gacgccccca
     1801 agcagcgccc agcggagccc agggccacgc ccctctgcgc actcgcacgc cggctctctg
     1861 cgccccggcc tgagcgcgtc gtggggcgag cccggggggc tgcgcgcggc gggcggcggc
     1921 ggcagcacca gcagcttcct gagttccccc tccgagtcct cgggctacgc cacgctgcac
     1981 tcggactcgc tgggctccgc gtccacagaa accagccaag tggcgcctgc ctaa
//
