LOCUS       sequence-200817-        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR160; Opto-GPR160; E8).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR160
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcacac taggaatgag aagaaaaaac
      301 acctgtcaaa atctcaacta catcctgctc aacctggccg tggccgacct cttcatggtg
      361 ttcgggggct tcaccaccac cctctacacc tctctgcacg ggtacttcgt ctttgggccc
      421 acgggctgca acctggaggg cttctttgcc accttgggcg gtgaaattgc actgtggtcc
      481 ttggtggtcc tggccatcga gcggtacgtg gtggtgtcta aaacaaccaa gctttcattt
      541 aagtgtcaaa aattatttta tttcgccatc atgggcgtcg ccttcacctg ggtcatggct
      601 ctggcctgtg ccgcgccccc cctcgtcggc tggtccaggt acatcccgga gggcatgcag
      661 tgctcgtgcg ggattgacta ctacacgccc cacgaggaaa ccaacaatga gtcgttcgtc
      721 atctacatgt tcgtggtcca cttcatcatc cccctgattg tcatattctt ctgctacgga
      781 aggataactt cctatatgaa tgaaactatc ttatattttc ctttttcatc ccactccagt
      841 tatactgtga gatctaaaaa acgcatggtg atcatcatgg tcatcgcttt cctaatctgc
      901 tggctgccct acgctggggt ggcgttctac atcttcaccc atcagggctc tgactttggc
      961 cccatcttca tgaccatccc ggctttcttt gccaagactt ctgccgtcta caaccccgtc
     1021 atctacatca tgatgaacaa gcagttccgc ttaaaagaca ttggattacc tttggatcca
     1081 tttgtcaact ggaagtgctg cttcattcca cttacaattc ctaatcttga gcaaattgaa
     1141 aagcctatat caataatgat ttgtacagaa accagccaag tggcgcctgc ctaa
//
