LOCUS       sequence-200818-        1758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR161; Opto-GPR161; E9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR161
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcttgt acaagaagtc ctacctcctc
      301 accctcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg ctgtacccca tggtgtaccc catgaagatc
      541 acagggaacc gggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggatt catcttccgc
      781 gtggccaggg tcaaggcacg caaggtgcac tgtggcacag tcgtcatcgt ggaggaggat
      841 gctcagagga ccgggaggaa gaactccagc acctccacct cctcttcagg cagcaggagg
      901 aatgcctttc agggtgtggt ctactcggcc aaccagtgca aagccctccg catggtgatc
      961 atcatggtca tcgctttcct aatctgctgg ctgccctacg ctggggtggc gttctacatc
     1021 ttcacccatc agggctctga ctttggcccc atcttcatga ccatcccggc tttctttgcc
     1081 aagacttctg ccgtctacaa ccccgtcatc tacatcatga tgaacaagca gttccgcaaa
     1141 gaactactgg gcatgtgctt tggggaccga tattatcggg aaccatttgt gcaacgacag
     1201 aggacttcca ggctgttcag catttccaac aggatcacag acctgggcct gtccccacac
     1261 ctcactgcgc tcatggcagg tggacagccc ctggggcaca gcagcagcac gggggacact
     1321 ggcttcagct gctcccagga ctcagggaca gatatgatgc tgcttgagga ctacacgtct
     1381 gatgacaacc ctccctctca ctgcacttgc ccacccaaga gaaggagctc ggtgacattt
     1441 gaggatgaag tggaacaaat caaagaagct gccaagaact cgattcttca tgtgaaagct
     1501 gaagtacaca agtccttgga cagttacgca gcaagcttgg ccaaagccat tgaggccgaa
     1561 gccaaaatca acttatttgg ggaggaggct ttgccagggg tcttggttac agcacggact
     1621 gtcccggggg gcggcttcgg gggccgccga ggcagcagaa ctcttgtgag ccagaggctg
     1681 cagttgcaga gcatcgaaga aggagatgtt ttagctgccg agcagagaac agaaaccagc
     1741 caagtggcgc ctgcctaa
//
