LOCUS       sequence-200819-        1950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR162; Opto-GPR162; E10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR162
FEATURES             Location/Qualifiers
     source          1..1950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcatct cggccaagca gcagaagcac
      301 aagccactca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg cgctggcccg tcaactaccg cctcagcaac
      541 gccaagaagc aggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaac actgtgggcc
      781 cggccccgga gggctcggca ggcccggaga gtggggggtg gtggggggac caaagcgggt
      841 gggccagggg ccttgggtac ccggccagct tttgaggtac cagccattgt ggtggaggat
      901 gcccgaggga agcggcggtc ctcgctggat ggctcggagt ctgccaagac atccctgcag
      961 cgcatggtga tcatcatggt catcgctttc ctaatctgct ggctgcccta cgctggggtg
     1021 gcgttctaca tcttcaccca tcagggctct gactttggcc ccatcttcat gaccatcccg
     1081 gctttctttg ccaagacttc tgccgtctac aaccccgtca tctacatcat gatgaacaag
     1141 cagttccgcg ccgacgtgcg cacagtgtgg gagcaatgcg tggccatcat gtctgaggag
     1201 gatggagatg acgatggggg ctgtgacgac tatgcagagg gccgagtttg caaagttcgc
     1261 tttgatgcta acggagccac aggaccaggg agccgggacc ccgcccaggt gaagctgctg
     1321 cctggaaggc acatgctgtt ccctcctctt gagagagtcc actacttaca ggtcccccta
     1381 tcccggcgtc tgtcccatga tgagacaaac atcttctcta cccctcggga accaggctcc
     1441 ttcctgcaca agtggtcatc ctctgatgac atccgggtcc tcccagccca gagccgggcc
     1501 ctcgggggtc ctcctgagta cctgggacaa agacacaggt tggaggacga ggaggacgag
     1561 gaagaggctg aaggtggggg gctggccagc cttcgccaat tcttggagag tggggttctg
     1621 gggtcaggtg ggggaccccc acggggtcct ggcttcttcc gggaggagat caccaccttc
     1681 atcgatgaga cacctctgcc ttctccgact gcctcaccag ggcactctcc tcgtcggccc
     1741 cggccactgg gcctctcacc ccgccgactc tcccttgggt cccctgagag cagagccgtt
     1801 ggacttcctt tgggactaag cgcagggagg cgctgctccc tgacgggggg tgaagaaagt
     1861 gcaagggctt ggggaggatc ctggggccca ggcaacccca tctttcccca gctgaccctg
     1921 acagaaacca gccaagtggc gcctgcctaa
//
