LOCUS       sequence-200824-        1278 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR182; Opto-GPR182; F3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR182
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcaact ggcgcggctc aggccgggca
      301 gggctgctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg accagcgcct ccccctcctg gcagcgttac
      541 cagcaccgag tgcggcgggc cgccatcatg ggcgtcgcct tcacctgggt catggctctg
      601 gcctgtgccg cgccccccct cgtcggctgg tccaggtaca tcccggaggg catgcagtgc
      661 tcgtgcggga ttgactacta cacgccccac gaggaaacca acaatgagtc gttcgtcatc
      721 tacatgttcg tggtccactt catcatcccc ctgattgtca tattcttctg ctacggactg
      781 acagcctgcc ggctgcggca gccaggacaa cccaagagcc ggcgccactg ccgcatggtg
      841 atcatcatgg tcatcgcttt cctaatctgc tggctgccct acgctggggt ggcgttctac
      901 atcttcaccc atcagggctc tgactttggc cccatcttca tgaccatccc ggctttcttt
      961 gccaagactt ctgccgtcta caaccccgtc atctacatca tgatgaacaa gcagttccgc
     1021 ggccggctcc tgaatgctgt agtccattac cttcctaagg accagaccaa ggcgggcaca
     1081 tgcgcctcct cttcctcctg ttccacccag cattccatca tcatcaccaa gggtgatagc
     1141 cagcctgctg cagcagcccc ccaccctgag ccaagcctga gctttcaggc acaccatttg
     1201 cttccaaata cttcccccat ctctcccact cagcctctta cacccagcac agaaaccagc
     1261 caagtggcgc ctgcctaa
//
