LOCUS       sequence-200826-        1398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-beta2AR
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcattg ccaagttcga gaggctacag
      301 actgtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg acatcgccat tcaagtacca gagcctgctg
      541 accaagaata aggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaag ggtgttccag
      781 gtggccaaaa ggcagctcca gaagatagac aaatctgagg gaagattcca ctccccaaac
      841 ctcggccagg tggagcagga tgggcggagt gggcacggac tccgaaggtc ctccaagttc
      901 tgcttgaagg agcacaaagc cctccgcatg gtgatcatca tggtcatcgc tttcctaatc
      961 tgctggctgc cctacgctgg ggtggcgttc tacatcttca cccatcaggg ctctgacttt
     1021 ggccccatct tcatgaccat cccggctttc tttgccaaga cttctgccgt ctacaacccc
     1081 gtcatctaca tcatgatgaa caagcagttc cgcattgcct tccaggagct tctatgcctc
     1141 cgcaggtcct cttcaaaagc ctatgggaac ggctactcca gcaacagtaa tggcaaaaca
     1201 gactacatgg gggaggcgag tggatgtcag ctggggcagg aaaaagaaag tgaacggctg
     1261 tgtgaggacc ccccaggcac ggaaagcttt gtgaactgtc aaggtactgt gcctagcctt
     1321 agccttgatt cccaagggag gaactgtagt acaaatgact caccgctgac agaaaccagc
     1381 caagtggcgc ctgcctaa
//
