LOCUS       sequence-200827-        1581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-alpha1AR
FEATURES             Location/Qualifiers
     source          1..1581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtag cctgtcaccg acacctgcac
      301 tcagtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg agctacccgc tgcgctaccc aaccatcgtc
      541 acccagagga gggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggacg cgtctacgtg
      781 gtggccaaga gggagagccg gggcctcaag tctggcctca agaccgacaa gtcggactcg
      841 gagcaagtga cgctccgcat ccatcggaaa aacgccccgg caggaggcag cgggatggcc
      901 agcgccaaga ccaagacgca cttctcagtg aggctcctca agttctcccg ggagaagaaa
      961 gcggcccgca tggtgatcat catggtcatc gctttcctaa tctgctggct gccctacgct
     1021 ggggtggcgt tctacatctt cacccatcag ggctctgact ttggccccat cttcatgacc
     1081 atcccggctt tctttgccaa gacttctgcc gtctacaacc ccgtcatcta catcatgatg
     1141 aacaagcagt tccgcaaggc ctttcagaat gtcttgagaa tccagtgtct ctgcagaaag
     1201 cagtcctcca aacatgccct gggctacacc ctgcacccgc ccagccaggc cgtggaaggg
     1261 caacacaagg acatggtgcg catccccgtg ggatcaagag aaaccttcta caggatctcc
     1321 aagacggatg gcgtttgtga atggaaattt ttctcttcca tgccccgtgg atctgccagg
     1381 attacagtgt ccaaagacca atcctcctgt accacagccc gggtgagaag taaaagcttt
     1441 ttgcaggtct gctgctgtgt agggccctca acccccagcc ttgacaagaa ccatcaagtt
     1501 ccaaccatta aggtccacac catctccctc agtgagaacg gggaggaagt cacagaaacc
     1561 agccaagtgg cgcctgccta a
//
