LOCUS       sequence-200829-        1434 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-A2AR; Opto-A2AR; F8; 719).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-A2AR
FEATURES             Location/Qualifiers
     source          1..1434
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtgt ggctcaacag caacctgcag
      301 aacgtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg cgcatcccgc tccggtacaa tggcttggtg
      541 accggcacga gggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggacg gatcttcctg
      781 gcggcgcgac gacagctgaa gcagatggag agccagcctc tgccggggga gcgggcacgg
      841 tccacactgc agaaggaggt ccatgctcgc atggtgatca tcatggtcat cgctttccta
      901 atctgctggc tgccctacgc tggggtggcg ttctacatct tcacccatca gggctctgac
      961 tttggcccca tcttcatgac catcccggct ttctttgcca agacttctgc cgtctacaac
     1021 cccgtcatct acatcatgat gaacaagcag ttccgccaga ccttccgcaa gatcattcgc
     1081 agccacgtcc tgaggcagca agaacctttc aaggcagctg gcaccagtgc ccgggtcttg
     1141 gcagctcatg gcagtgacgg agagcaggtc agcctccggc tcaacggcca cccgccagga
     1201 gtgtgggcca acggcagtgc tccccaccct gagcggaggc ccaatggcta tgccctgggg
     1261 ctggtgagtg gagggagtgc ccaagagtcc caggggaaca cgggcctccc agacgtggag
     1321 ctccttagcc atgagctcaa gggagtgtgc ccagagcccc ctggcctaga tgaccccctg
     1381 gcccaggatg gagcaggagt gtccacagaa accagccaag tggcgcctgc ctaa
//
