LOCUS       sequence-200831-        1506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-D2R; Opto-D2R; F10; 721).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-D2R
FEATURES             Location/Qualifiers
     source          1..1506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcgtat cacgagagaa ggctttgcag
      301 accaccctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg gccatgccta tgttgtataa cacacgctac
      541 agctccaagc gccgagccat catgggcgtc gccttcacct gggtcatggc tctggcctgt
      601 gccgcgcccc ccctcgtcgg ctggtccagg tacatcccgg agggcatgca gtgctcgtgc
      661 gggattgact actacacgcc ccacgaggaa accaacaatg agtcgttcgt catctacatg
      721 ttcgtggtcc acttcatcat ccccctgatt gtcatattct tctgctacgg aaaaatctac
      781 atcgttctcc gcaagcgtcg gaagcgggtc aacaccaagc gtagcagccg agctttcaga
      841 gccaacctga aaacaccact caagggcaac tgtacccacc ctgaggacat gaaactctgc
      901 accgttatca tgaagtctaa tgggagtttc ccagtgaaca ggcggagaat ggatgctgcc
      961 cgccgagctc aggagctgga aatggagatg ctgtcaagca ccagcccccc agagaggacc
     1021 cggtatagcc ccatccctcc cagtcaccac cagctcactc tccccgatcc atcccaccac
     1081 ggtctacata gcaaccctga cagtcctgcc aaaccagaaa agaatgggca tgccaagatt
     1141 gtcaatccca ggattgccaa gttctttgag atccagacca tgcccaatgg caaaacccgg
     1201 acctccctta agacgatgag ccgcaggaag ctctcccagc agaaggagaa gaaagccact
     1261 cgcatggtga tcatcatggt catcgctttc ctaatctgct ggctgcccta cgctggggtg
     1321 gcgttctaca tcttcaccca tcagggctct gactttggcc ccatcttcat gaccatcccg
     1381 gctttctttg ccaagacttc tgccgtctac aaccccgtca tctacatcat gatgaacaag
     1441 cagttccgca aggccttcat gaagatcctg cactgcacag aaaccagcca agtggcgcct
     1501 gcctaa
//
