LOCUS       sequence-200833-        1578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-M2R; Opto-M2R; F12; 723).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-M2R
FEATURES             Location/Qualifiers
     source          1..1578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcatta aagtcaaccg ccacctccag
      301 accgtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg acaaaacctc tgacctaccc agtcaagcgg
      541 accacaaaaa tggccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaca catatcccga
      781 gccagcaaga gcaggataaa gaaggacaag aaggagcctg ttgccaacca agaccccgtt
      841 tctccaagtc tggtacaagg aaggatagtg aagccaaaca ataacaacat gcccagcagt
      901 gacgatggcc tggagcacaa caaaatccag aatggcaaag cccccaggga tcctgtgact
      961 gaaaactgtg ttcagggaga ggagaaggag agctccaatg actccacctc agtcagtgct
     1021 gttgcctcta atatgagaga tgatgaaata acccaggatg aaaacacagt ttccacttcc
     1081 ctgggccatt ccaaagatga gaactctaag caaacatgca tcagaattgg caccaagacc
     1141 ccaaaaagtg actcatgtac cccaactaat accaccgtgg aggtagtggg gtcctcaggt
     1201 cagaatggag atgaaaagca gaatattgta gcccgcaaga ttgtgaagat gactaagcag
     1261 cctgcaaaaa agaagcctcc tccttcccgg gaaaagaaag tcacccgcat ggtgatcatc
     1321 atggtcatcg ctttcctaat ctgctggctg ccctacgctg gggtggcgtt ctacatcttc
     1381 acccatcagg gctctgactt tggccccatc ttcatgacca tcccggcttt ctttgccaag
     1441 acttctgccg tctacaaccc cgtcatctac atcatgatga acaagcagtt ccgcaaaacc
     1501 tttaaacacc ttctcatgtg tcattataag aacataggcg ctacaaggac agaaaccagc
     1561 caagtggcgc ctgcctaa
//
