LOCUS       sequence-200834-        1815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric putative G-protein coupled receptor 
            (Rhodopsin-M3R; Opto-M3R; H1; 724).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-M3R
FEATURES             Location/Qualifiers
     source          1..1815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcttta aggtcaacaa gcagctgaag
      301 acggtcctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg acgaggccgc tcacgtaccg agccaaacga
      541 acaacaaaga gagccatcat gggcgtcgcc ttcacctggg tcatggctct ggcctgtgcc
      601 gcgccccccc tcgtcggctg gtccaggtac atcccggagg gcatgcagtg ctcgtgcggg
      661 attgactact acacgcccca cgaggaaacc aacaatgagt cgttcgtcat ctacatgttc
      721 gtggtccact tcatcatccc cctgattgtc atattcttct gctacggaag gatctataag
      781 gaaactgaaa agcgtaccaa agagcttgct ggcctgcaag cctctgggac agaggcagag
      841 acagaaaact ttgtccaccc cacgggcagt tctcgaagct gcagcagtta cgaacttcaa
      901 cagcaaagca tgaaacgctc caacaggagg aagtatggcc gctgccactt ctggttcaca
      961 accaagagct ggaaacccag ctccgagcag atggaccaag accacagcag cagtgacagt
     1021 tggaacaaca atgatgctgc tgcctccctg gagaactccg cctcctccga cgaggaggac
     1081 attggctccg agacgagagc catctactcc atcgtgctca agcttccggg tcacagcacc
     1141 atcctcaact ccaccaagtt accctcatcg gacaacctgc aggtgcctga ggaggagctg
     1201 gggatggtgg acttggagag gaaagccgac aagctgcagg cccagaagag cgtggacgat
     1261 ggaggcagtt ttccaaaaag cttctccaag cttcccatcc agctagagtc agccgtggac
     1321 acagctaaga cttctgacgt caactcctca gtgggtaaga gcacggccac tctacctctg
     1381 tccttcaagg aagccactct ggccaagagg tttgctctga agaccagaag tcagatcact
     1441 aagcggaaaa ggatgtccct ggtcaaggag aagaaagcgg cccgcatggt gatcatcatg
     1501 gtcatcgctt tcctaatctg ctggctgccc tacgctgggg tggcgttcta catcttcacc
     1561 catcagggct ctgactttgg ccccatcttc atgaccatcc cggctttctt tgccaagact
     1621 tctgccgtct acaaccccgt catctacatc atgatgaaca agcagttccg caccactttc
     1681 aagatgctgc tgctgtgcca gtgtgacaaa aaaaagaggc gcaagcagca gtaccagcag
     1741 agacagtcgg tcatttttca caagcgcgca cccgagcagg ccttgacaga aaccagccaa
     1801 gtggcgcctg cctaa
//
