LOCUS       sequence-200883-        5255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under 
            control of the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Nelson EV, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Ruedas JB, 
            Connor JH, Ebihara H, Muhlberger E
  TITLE     An RNA polymerase II-driven Ebola virus minigenome system as an 
            advanced tool for antiviral drug screening.
  JOURNAL   Antiviral Res. 2017 Aug 12;146:21-27. doi: 
            10.1016/j.antiviral.2017.08.005.
  PUBMED    28807685
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antiviral 
            Res."; date: "2017-08-12"; volume: "146:21-27. doi"; pages: " 
            10.1016/j.antiviral.2017.08.005"
COMMENT     Sequence Label: pCAGGS_VP35_EBOV
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     829..851
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     913..932
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2273..2292)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2338..2393
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2392..2411)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2754..2770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2754..2770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2767..2789)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2778..2794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2802..2808,2809..2826,2827..2832))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2927..3122
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2973..3108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3128..3262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3165..3184)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3219..3238
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3330..3347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3501..4089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3581..3600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4260..5051,5052..5120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4883..4902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5121..5225)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gccggggagg gctcggggga ggggcgcggc ggcccccgga
      541 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
      601 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
      661 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
      721 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
      781 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
      841 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
      901 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc
      961 tcgagggtac ccccggggcg gccgcgagct cactagtgga tccaaacctt catcatcctt
     1021 acgtcaattg aattctctag cactcgaagc ttattgtctt caatgtaaaa gaaaagctgg
     1081 tctaacaaga tgacaactag aacaaagggc aggggccata ctgcggccac gactcaaaac
     1141 gacagaatgc caggccctga gctttcgggc tggatctctg agcagctaat gaccggaaga
     1201 attcctgtaa gcgacatctt ctgtgatatt gagaacaatc caggattatg ctacgcatcc
     1261 caaatgcaac aaacgaagcc aaacccgaag acgcgcaaca gtcaaaccca aacggaccca
     1321 atttgcaatc atagttttga ggaggtagta caaacattgg cttcattggc tactgttgtg
     1381 caacaacaaa ccatcgcatc agaatcatta gaacaacgca ttacgagtct tgagaatggt
     1441 ctaaagccag tttatgatat ggcaaaaaca atctcctcat tgaacagggt ttgtgctgag
     1501 atggttgcaa aatatgatct tctggtgatg acaaccggtc gggcaacagc aaccgctgcg
     1561 gcaactgagg cttattgggc cgaacatggt caaccaccac ctggaccatc actttatgaa
     1621 gaaagtgcga ttcggggtaa gattgaatct agagatgaga ccgtccctca aagtgttagg
     1681 gaggcattca acaatctaaa cagtaccact tcactaactg aggaaaattt tgggaaacct
     1741 gacatttcgg caaaggattt gagaaacatt atgtatgatc acttgcctgg ttttggaact
     1801 gctttccacc aattagtaca agtgatttgt aaattgggaa aagatagcaa ctcattggac
     1861 atcattcatg ctgagttcca ggccagcctg gctgaaggag actctcctca atgtgcccta
     1921 attcaaatta caaaaagagt tccaatcttc caagatgctg ctccacctgt catccacatc
     1981 cgctctcgag gtgacattcc ccgagcttgc cagaaaagct tgcgtccagt cccaccatcg
     2041 cccaagattg atcgaggttg ggtatgtgtt tttcagcttc aagatggtaa aacacttgga
     2101 ctcaaaattt gagccaatct cccttccctc cgaaagaggc gaataatagc agaggcttca
     2161 actgctgaac tatagggtac gttacattaa tgatacactt gtgagtatca gccctggata
     2221 atataagtca attaaacgac caagataaaa ttgttcatat ctcgctagca gatctttttc
     2281 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     2341 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     2401 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     2461 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     2521 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     2581 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     2641 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     2701 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     2761 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2821 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2881 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     2941 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3001 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3061 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3121 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3241 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     3301 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3421 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3481 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3661 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3721 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3781 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3841 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3901 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3961 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4021 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4081 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4141 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4201 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4261 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4321 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4381 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4441 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4501 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4561 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4621 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4681 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4741 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4801 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4861 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4921 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4981 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5041 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5101 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5161 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5221 ccgcgcacat ttccccgaaa agtgccacct gggtc
//
