LOCUS       sequence-200884-        6357 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under 
            control of the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Nelson EV, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Ruedas JB, 
            Connor JH, Ebihara H, Muhlberger E
  TITLE     An RNA polymerase II-driven Ebola virus minigenome system as an 
            advanced tool for antiviral drug screening.
  JOURNAL   Antiviral Res. 2017 Aug 12;146:21-27. doi: 
            10.1016/j.antiviral.2017.08.005.
  PUBMED    28807685
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antiviral 
            Res."; date: "2017-08-12"; volume: "146:21-27. doi"; pages: " 
            10.1016/j.antiviral.2017.08.005"
COMMENT     Sequence Label: pCAGGS_NP_EBOV
FEATURES             Location/Qualifiers
     source          1..6357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     818..840
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     902..921
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2555..2566
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3375..3394)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3440..3495
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3494..3513)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3856..3872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3856..3872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3869..3891)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3880..3896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3904..3910,3911..3928,3929..3934))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3949..3970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4029..4224
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4075..4210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4230..4364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4267..4286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4321..4340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4432..4449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4603..5191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4683..4702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5362..6153,6154..6222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5985..6004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6223..6327)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
      541 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
      601 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
      661 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
      721 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
      781 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
      841 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
      901 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagggtacc
      961 cccggggcgg ccgcgagctc actagtggat ccacaaaatt ccgagtatgg attctcgtcc
     1021 tcagaaaatc tggatggcgc cgagtctcac tgaatctgac atggattacc acaagatctt
     1081 gacagcaggt ctgtccgttc aacaggggat tgttcggcaa agagtcatcc cagtgtatca
     1141 agtaaacaat cttgaagaaa tttgccaact tatcatacag gcctttgaag caggtgttga
     1201 ttttcaagag agtgcggaca gtttccttct catgctttgt cttcatcatg cgtaccaggg
     1261 agattacaaa cttttcttgg aaagtggcgc agtcaagtat ttggaagggc acgggttccg
     1321 ttttgaagtc aagaagcgtg atggagtgaa gcgccttgag gaattgctgc cagcagtatc
     1381 tagtggaaaa aacattaaga gaacacttgc tgccatgccg gaagaggaga caactgaagc
     1441 taatgccggt cagtttctct ccttcgcaag tctattcctt ccgaaattgg tagtaggaga
     1501 aaaggcttgc cttgagaagg ttcaaaggca aattcaagta catgcagagc aaggactgat
     1561 acaatatcca acagcttggc aatcagtagg acacatgatg gtgattttcc gtttgatgcg
     1621 aacaaatttt ctgatcaaat ttctcctaat acaccaaggg atgcacatgg ttgccgggca
     1681 tgatgccaac gatgctgtga tttcaaattc agtggctcaa gctcgttttt caggcttatt
     1741 gattgtcaaa acagtacttg atcatatcct acaaaagaca gaacgaggag ttcgtctcca
     1801 tcctcttgca aggaccgcca aggtaaaaaa tgaggtgaac tcctttaagg ctgcactcag
     1861 ctccctggcc aagcatggag agtatgctcc tttcgcccga cttttgaacc tttctggagt
     1921 aaataatctt gagcatggtc ttttccctca actatcggca attgcactcg gagtcgccac
     1981 agcacacggg agtaccctcg caggagtaaa tgttggagaa cagtatcaac aactcagaga
     2041 ggctgccact gaggctgaga agcaactcca acaatatgca gagtctcgcg aacttgacca
     2101 tcttggactt gatgatcagg aaaagaaaat tcttatgaac ttccatcaga aaaagaacga
     2161 aatcagcttc cagcaaacaa acgctatggt aactctaaga aaagagcgcc tggccaagct
     2221 gacagaagct atcactgctg cgtcactgcc caaaacaagt ggacattacg atgatgatga
     2281 cgacattccc tttccaggac ccatcaatga tgacgacaat cctggccatc aagatgatga
     2341 tccgactgac tcacaggata cgaccattcc cgatgtggtg gttgatcccg atgatggaag
     2401 ctacggcgaa taccagagtt actcggaaaa cggcatgaat gcaccagatg acttggtcct
     2461 attcgatcta gacgaggacg acgaggacac taagccagtg cctaatagat cgaccaaggg
     2521 tggacaacag aagaacagtc aaaagggcca gcatatagag ggcagacaga cacaatccag
     2581 gccaattcaa aatgtcccag gccctcacag aacaatccac cacgccagtg cgccactcac
     2641 ggacaatgac agaagaaatg aaccctccgg ctcaaccagc cctcgcatgc tgacaccaat
     2701 taacgaagag gcagacccac tggacgatgc cgacgacgag acgtctagcc ttccgccctt
     2761 ggagtcagat gatgaagagc aggacaggga cggaacttcc aaccgcacac ccactgtcgc
     2821 cccaccggct cccgtataca gagatcactc tgaaaagaaa gaactcccgc aagacgagca
     2881 acaagatcag gaccacactc aagaggccag gaaccaggac agtgacaaca cccagtcaga
     2941 acactctttt gaggagatgt atcgccacat tctaagatca caggggccat ttgatgctgt
     3001 tttgtattat catatgatga aggatgagcc tgtagttttc agtaccagtg atggcaaaga
     3061 gtacacgtat ccagactccc ttgaagagga atatccacca tggctcactg aaaaagaggc
     3121 tatgaatgaa gagaatagat ttgttacatt ggatggtcaa caattttatt ggccggtgat
     3181 gaatcacaag aataaattca tggcaatcct gcaacatcat cagtgaatga gcatggaaca
     3241 atgggatgat tcaaccgaca aatagctaac attaagtagt caaggaacga aaacaggaag
     3301 aatttttgat gtctaaggtg tgaattatta tcacaataaa agtgattctt atttttgaat
     3361 ttaaagctag cagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     3421 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     3481 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     3541 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     3601 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     3661 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     3721 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     3781 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     3841 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3901 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3961 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4021 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4081 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4141 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4201 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     4261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4321 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     4381 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4441 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4501 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5041 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5281 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5341 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5401 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5461 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5521 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5581 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5641 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5701 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5761 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5821 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5881 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5941 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6001 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6061 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6121 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6181 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6241 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6301 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtc
//
