LOCUS       sequence-200885-        5796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under 
            control of the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Nelson EV, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Ruedas JB, 
            Connor JH, Ebihara H, Muhlberger E
  TITLE     An RNA polymerase II-driven Ebola virus minigenome system as an 
            advanced tool for antiviral drug screening.
  JOURNAL   Antiviral Res. 2017 Aug 12;146:21-27. doi: 
            10.1016/j.antiviral.2017.08.005.
  PUBMED    28807685
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antiviral 
            Res."; date: "2017-08-12"; volume: "146:21-27. doi"; pages: " 
            10.1016/j.antiviral.2017.08.005"
COMMENT     Sequence Label: pCAGGS_VP30_EBOV
FEATURES             Location/Qualifiers
     source          1..5796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     825..847
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     909..928
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2814..2833)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2879..2934
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2933..2952)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3295..3311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3295..3311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3308..3330)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3319..3335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3343..3349,3350..3367,3368..3373))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3388..3409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3468..3663
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3514..3649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3669..3803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3706..3725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3760..3779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3871..3888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4042..4630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4122..4141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4801..5592,5593..5661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5424..5443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5662..5766)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
      541 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
      601 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
      661 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
      721 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
      781 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
      841 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
      901 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattccccgg
      961 ggagctcact agtggatccg cgaaccggta gagtttagtt gcaacctaac acacataaag
     1021 cattggtcaa aaagtcaata gaaatttaaa cagtgagtgg agacaacttt taaatggaag
     1081 cttcatatga gagaggacgc ccacgagctg ccagacagca ttcaagggat ggacacgacc
     1141 accatgttcg agcacgatca tcatccagag agaattatcg aggtgagtac cgtcaatcaa
     1201 ggagcgcctc acaagtgcgc gttcctactg tatttcataa gaagagagtt gaaccattaa
     1261 cagttcctcc agcacctaaa gacatatgtc cgaccttgaa aaaaggattt ttgtgtgaca
     1321 gtagtttttg caaaaaagat caccagttgg agagtttaac tgatagggaa ttactcctac
     1381 taatcgcccg taagacttgt ggatcagtag aacaacaatt aaatataact gcacccaagg
     1441 actcgcgctt agcaaatcca acggctgatg atttccagca agaggaaggt ccaaaaatta
     1501 ccttgttgac actgatcaag acggcagaac actgggcgag acaagacatc agaaccatag
     1561 aggattcaaa attaagagca ttgttgactc tatgtgctgt gatgacgagg aaattctcaa
     1621 aatcccagct gagtctttta tgtgagacac acctaaggcg cgaggggctt gggcaagatc
     1681 aggcagaacc cgttctcgaa gtatatcaac gattacacag tgataaagga ggcagttttg
     1741 aagctgcact atggcaacaa tgggaccgac aatccctaat tatgtttatc actgcattct
     1801 tgaatattgc tctccagtta ccgtgtgaaa gttctgctgt cgttgtttca gggttaagaa
     1861 cattggttcc tcaatcagat aatgaggaag cttcaaccaa cccggggaca tgctcatggt
     1921 ctgatgaggg taccccttaa taaggctgac taaaacacta tataaccttc tacttgatca
     1981 caatactccg tatacctatc atcatatatt taatcaagac gatatccttt aaaacttatt
     2041 cagtactata atcactctcg tttcaaatta ataagatgtg catgattgcc ctaatatatg
     2101 aagaggtatg atacaaccct aacagtgatc aaagaaaatc ataatctcgt atcgctcgaa
     2161 atataacctg ccaagcatac ctcttgcaca aagtgattct tgtacacaaa taatgtttta
     2221 ctctacagga ggtagcaacg atccatccca tcaaaaaata agtatttcat gacttactaa
     2281 tgatctctta aaatattaag aaaaactgac ggaacataaa ttctttatgc ttcaagctgt
     2341 ggaggaggtg tttggtattg gctattgtta tattacaatc aataacaagc ttgtaaaaat
     2401 attgttcttg tttcaagagg tagattgtga ccggaaatgc taaactaatg atgaagatta
     2461 atgcggaggt ctgataagaa taaaccttat tattcagatt aggccccaag aggcattctt
     2521 catctccttt tagcaaagta ctatttcagg gtagtccaat tagtggcacg tcttttagct
     2581 gtatatcagt cgcccctgag atacgccaca aaagtgtctc taagctaaat tggtctgtac
     2641 acatcccata cattgtatta ggggcaataa tatctaattg aacttagccg tttaaaattt
     2701 agtgcataaa tctgggctaa caccaccagg tcaactccat tggctgaaaa gaagcttacc
     2761 tgcagctcga gggtaccccc ggggcggccg cgagctctta attagctagc agatcttttt
     2821 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     2881 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2941 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     3001 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     3061 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     3121 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     3181 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     3241 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     3301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
     3481 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3661 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3781 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
     3841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5701 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5761 tccgcgcaca tttccccgaa aagtgccacc tgggtc
//
