LOCUS       sequence-200891-        3674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector containing TIAL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Meyer C, Garzia A, Mazzola M, Gerstberger S, Molina H, Tuschl T
  TITLE     The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated 
            Stress Response and Cell Cycle Progression.
  JOURNAL   Mol Cell. 2018 Feb 15;69(4):622-635.e6. doi: 
            10.1016/j.molcel.2018.01.011.
  PUBMED    29429924
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.01"; journalName: "Mol Cell"; date: 
            "2018-02-15- 15"; volume: "69"; issue: "4"; pages: "622-635.e6"
COMMENT     Sequence Label: pENTR4_TIAL1
FEATURES             Location/Qualifiers
     source          1..3674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1799..1898)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1915..1934)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1916..1934)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1939..1955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1939..1955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2057..2076)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2068..2877
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2135..2154)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3513..3532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atgatggaag acgacgggca gccccggact ctatacgtag gtaacctttc
      721 cagagatgtg acagaagtcc ttatacttca gttgttcagt cagattggac cctgtaaaag
      781 ctgtaaaatg ataacagagc atacaagcaa tgacccatat tgctttgtgg aattttatga
      841 acacagagat gcagctgctg cattagctgc tatgaatggg agaaaaattt tgggaaagga
      901 ggtcaaagta aactgggcaa ccacaccaag tagccagaaa aaagatactt ccaatcactt
      961 ccatgtgttt gttggggatt tgagtccaga aattacaaca gaagatatca aatcagcatt
     1021 tgcccccttt ggtaaaatat cggatgcccg ggtagttaaa gacatggcaa ctggaaaatc
     1081 caaaggctat ggttttgtat ctttttataa caaactggat gcagaaaatg cgattgtgca
     1141 tatgggcggt cagtggttgg gtggtcgtca aatccgaacc aattgggcca ctcgtaaacc
     1201 acctgcacct aaaagtacac aagaaaacaa cactaagcag ttgagatttg aagatgtagt
     1261 aaaccagtca agtccaaaaa attgtactgt gtactgtgga ggaattgcgt ctgggttaac
     1321 agatcagctt atgagacaga cattctcacc atttggacaa attatggaaa taagagtttt
     1381 cccagaaaag ggctattcat ttgtcagatt ttcaacccat gaaagtgcag cccatgccat
     1441 tgtttcggtg aacggtacta cgattgaagg acatgtggtt aaatgctatt ggggtaaaga
     1501 atctcctgat atgactaaaa acttccaaca ggttgactat agtcaatggg gccaatggag
     1561 ccaagtgtat ggaaacccac aacagtatgg acagtatatg gcaaatgggt ggcaagtacc
     1621 gccttatgga gtatacgggc aaccatggaa tcaacaagga tttggagtag atcaatcacc
     1681 ttctgctgct tggatgggtg gatttggtgc tcagcctccc caaggacaag ctcctccccc
     1741 tgtaatacct cctcctaacc aagccggata tggtatggca agttaccaaa cacagtgaac
     1801 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1861 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1921 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1981 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct
     2041 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2101 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2161 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2221 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2281 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2341 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2401 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2461 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2521 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2581 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2641 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2701 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2761 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2821 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2881 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2941 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgtt
//
