LOCUS       sequence-200904-        5526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fast indicator for synaptic glutamate imaging (synapsin promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert
            JS, Geeves M, Oertner TG, Torok K
  TITLE     Ultrafast glutamate sensors resolve high-frequency release at 
            Schaffer collateral synapses.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 
            10.1073/pnas.1720648115.
  PUBMED    29735711
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 
            10.1073/pnas.1720648115."
COMMENT     Sequence Label: pCI syn iGluf
FEATURES             Location/Qualifiers
     source          1..5526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..533
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          570..702
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     685..704
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        747..765
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     856..915
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1884..1905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2011..2032)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2272..2291)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2497..2526
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2530..2676
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    2703..2824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2714..2733)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2768..2787
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3005..3460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3092..3111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3302..3323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3527..3546)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3646..3668
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3706..3724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3792..3896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3897..3965,3966..4757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4115..4134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4928..5516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5417..5436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgcggcctt ttacggttcc tggcctttgc tggccttttg ctcacatggc tcgacagatc
       61 taatctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg
      121 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc
      181 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt
      241 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc
      301 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc
      361 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga
      421 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc
      481 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagctgcagc
      541 taggaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
      601 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
      661 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca
      721 attacagctc ttaaggctag agtacttaat acgactcact ataggctagc gctaccggac
      781 tcaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      841 ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt
      901 ccaggttcca ctggtgacag atctgccgca ggcagcacgc tggacaaaat cgccaaaaac
      961 ggtgtgattg tcgtcggtca ccgtgattct tcagtgcctt tctcttatta cgacaatcag
     1021 caaaaagtgg tgggttactc gcaggattac tccaacgcca ttgttgaagc agtgaaaaag
     1081 aaactcaaca aaccggactt gcaggtaaaa ctgattccga ttacctcaca aaaccgtatt
     1141 ccactgctgc aaaacggcac tttcgatttt gaatgtggtt ctaccaccaa caacgtcgaa
     1201 cgccaaaaac aggcggcttt ctctgacact attttcgtgg tcggtacgcg cctgttgacc
     1261 aaaaagggtg gcgatatcaa agattttgcc aacctgaaag acaaagccgt agtcgtcact
     1321 tccggcacta cctctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg
     1381 cgcatcatca gcgccaaaga tcacggtgac tctttccgca ccctggaaag cggtcgtgcc
     1441 gttgccttta tgatggatga cgtgctgctg gccggtgaac gtgcgaaagc gaagaaacca
     1501 gacaactggg aaatcgtcgg caagccgcag tctcaggagg cctacggttg tatgttgcgt
     1561 aaagatgatc cgcagttcaa aaagctgatg gatgacacca tcgctcaggt gcagacctcc
     1621 ggtgaagcgg aaaaatggtt tgataagtgg ttcaaaaatc caattctggt gagccacaac
     1681 gtctatatca tggccgacaa gcagaggaac ggcatcaagg cgaacttcaa gatccgccac
     1741 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
     1801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa actttcgaaa
     1861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1921 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
     1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2041 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
     2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2221 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     2281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcataagct ggagtacaac
     2401 tttaacaacc ccctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa
     2461 gaaccgaatg acaaggcact gaaactgcag gtcgacgaac aaaaactcat ctcagaagag
     2521 gatctgaatg ctgtgggcca ggacacgcag gaggtcatcg tggtgccaca ctccttgccc
     2581 tttaaggtgg tggtgatctc agccatcctg gccctggtgg tgctcaccat catctccctt
     2641 atcatcctca tcatgctttg gcagaagaag ccacgttagg cggccgcttc gagcagacat
     2701 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2761 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2821 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     2881 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgggctggcg
     2941 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3001 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3421 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     3481 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     3541 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3601 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3661 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     3721 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     3781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3901 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3961 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4021 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4081 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4141 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4201 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4261 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4321 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4381 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4441 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4501 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4561 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4621 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4681 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4741 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4801 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5041 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5521 ccagca
//
