LOCUS       sequence-20095-e         588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Miller M, Shuman JD, Sebastian T, Dauter Z, Johnson PF
  TITLE     Structural basis for DNA recognition by the basic region leucine 
            zipper transcription factor CCAAT/enhancer-binding protein alpha.
  JOURNAL   J Biol Chem. 2003 Apr 25;278(17):15178-84. doi: 
            10.1074/jbc.M300417200. Epub 2003 Feb 10.
  PUBMED    12578822
REFERENCE   2  (bases 1 to 588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M300417200"; journalName: "J Biol Chem"; date: 
            "2003-04-25- 25"; volume: "278"; issue: "17"; pages: "15178-84"
COMMENT     Sequence Label: pcDNA3.1(-) rat C/EBP alpha
FEATURES             Location/Qualifiers
     source          1..588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agagctctct ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagactc gagcggccgc
      121 cactgtgctg gatatctgca gaattcgcgc caccatggag tcggccgact tctacgaggc
      181 ggagccgcgg cccccgatga gcagccacct ccagagcccc ccgcacgcgc ccagcaacgc
      241 cgcctttggc tttccccggg gcgcgggccc cgcgccgccc ccagccccac ctgccgcccc
      301 ggagccgctg ggcggcatct gcgagcacga gacgtctata gacatcagcg cctacatcga
      361 cccggccgcc ttcaacgacg agttcctggc cgacctcttc cagcacagcc ggcagcagga
      421 gaaggccaag gcggcggcgg gccccgcggg tggcggcggt gactttgact acccgggcgc
      481 cccggcgggc cccggcggtg cggtcatgtc cgcgggggcg cacggacccc ctcccggcta
      541 ctgctgtgcg gcggccggct acctggacgg aaagctggag cccctgta
//
