LOCUS       sequence-200988-        6041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse dopamine receptor subtype 1 with EGFP fused to the 
            C-terminus of the receptor. Localizes to primary cilia..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Domire JS, Green JA, Lee KG, Johnson AD, Askwith CC, Mykytyn K
  TITLE     Dopamine receptor 1 localizes to neuronal cilia in a dynamic process
            that requires the Bardet-Biedl syndrome proteins.
  JOURNAL   Cell Mol Life Sci. 2011 Sep;68(17):2951-60. doi: 
            10.1007/s00018-010-0603-4. Epub 2010 Dec 9.
  PUBMED    21152952
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-010-0603-4"; journalName: "Cell Mol Life Sci"; date:
            "2011-09"; volume: "68"; issue: "17"; pages: "2951-60"
COMMENT     Sequence Label: pEGFP-N-Drd1
FEATURES             Location/Qualifiers
     source          1..6041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2070..2079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2076..2078,2079..2081,2082..2795)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2076..2078,2079..2081,2082..2792)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2121..2142)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2382..2401)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2729..2750
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2918..3039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2955..2974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3009..3028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3046..3501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3183..3204)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3395..3414
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3528..3632
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3634..3991
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3634..3825
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3657..3677)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3842..3977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4026..4820
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4026..4820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4080..4099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4690..4709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5008..5027)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5052..5099
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5428..6016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5917..5936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
       61 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      661 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagatg gctcctaaca
      721 cttctaccat ggatgagact ggcctgccag tggagaggga cttctccttt cgcatcctca
      781 cagcctgttt tctgtccctg cttatcctgt ccactctctt agggaatacc cttgtctgtg
      841 ccgctgtcat caggtttcga cacctgcggt ccaaggtgac caacttcttt gtcatctctt
      901 tagctgtgtc agatctcttg gtggctgtct tggtcatgcc ctggaaagct gtggctgaga
      961 ttgctggctt ttggcccttt gggtcctttt gtaacatttg ggtagccttt gacatcatgt
     1021 gctccacggc atccatcctt aacctctgtg tgatcagcgt ggacaggtat tgggctatct
     1081 ccagcccttt ccagtatgag aggaagatga ctccgaaggc agccttcatc ctgattagcg
     1141 tagcatggac tttgtctgtt ctcatatcct tcattccagt gcagctaagc tggcacaagg
     1201 caaaacctac atggcccttg gatggcaatt ttacttccct ggaagatgcc gaggatgaca
     1261 actgtgacac gaggttgagc aggacatacg ccatttcatc ctccctcata agcttttaca
     1321 tccccgtagc cattatgatc gtcacttaca ccagtatcta caggattgcc cagaagcaaa
     1381 tccggcgcat ctcagctttg gagagggcag cagtccatgc caagaactgc cagaccacca
     1441 caggtaatgg aaaccctgtc gaatgctctc aatcagaaag ttcctttaag atgtccttta
     1501 agagggagac taaagtcctg aagacactgt ctgtgatcat gggggtattc gtgtgctgct
     1561 ggctcccttt cttcatttcg aactgtatgg tgcccttctg tggctctgag gagacccagc
     1621 cattctgcat tgattccatc accttcgatg tatttgtgtg gtttggctgg gcgaattcct
     1681 ccctgaaccc cattatttat gcttttaatg ccgattttca gaaggcattc tcgaccctct
     1741 taggatgcta tagactctgc cctacaacga ataatgccat agagactgta agcatcaaca
     1801 acaacggggc tgtgatgttt tccagccacc atgagccccg aggctccatc tccaaggact
     1861 gtaatctggt ttacctgatc cctcatgctg tgggctcctc tgaggacctg aagagggagg
     1921 aggccggtgg catacctaag ccactggaga agctgtcccc ggccttatcg gtcatattgg
     1981 actatgacac cgatgtctct ctagaaaaga tccaacccgt tacccacagc ggacagcatt
     2041 caaccggtac cgcgggcccg ggatccatcg ccaccatggt gagcaagggc gaggagctgt
     2101 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2161 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2221 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     2281 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2341 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2401 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2461 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2521 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2581 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2641 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2701 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2761 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat
     2821 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2881 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     3061 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3121 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3181 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3241 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3301 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3361 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3421 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3481 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3601 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     3661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3901 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3961 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     4021 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4081 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4141 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4201 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4261 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4321 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4381 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4441 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4501 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     4561 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4621 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4681 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4741 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4801 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4861 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     4921 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     4981 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     5041 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     5101 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     5161 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     5221 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     5281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta c
//
