LOCUS       sequence-200994-        3889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Caspase-6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Seaman JE, Julien O, Lee PS, Rettenmaier TJ, Thomsen ND, Wells JA
  TITLE     Cacidases: caspases can cleave after aspartate, glutamate and 
            phosphoserine residues.
  JOURNAL   Cell Death Differ. 2016 Oct;23(10):1717-26. doi: 
            10.1038/cdd.2016.62. Epub 2016 Jul 1.
  PUBMED    27367566
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2016.62"; journalName: "Cell Death Differ"; date: 
            "2016-10"; volume: "23"; issue: "10"; pages: "1717-26"
COMMENT     Sequence Label: Caspase-6
FEATURES             Location/Qualifiers
     source          1..3889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             390..407
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(460..478)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      474..521
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      558..1013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(645..664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     855..876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1039..1143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1144..1212,1213..2004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1362..1381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2175..2763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2664..2683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2917..2934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2949..3088
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3014..3033)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3174..3196
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3190..3381)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atggcagcct ccgtttacac gctgcctgct ggagctgact
      121 tcctcatgtg ttactctgtt gcagaaggat attattctca ccgggaaact gtgaacggct
      181 catggtacat tcaagatttg tgtgagatgt tgggaaaata tggctcctcc ttagagttca
      241 cagaactcct cacactggtg aacaggaaag tttctcagcg ccgagtggac ttttgcaaag
      301 acccaagtgc aattggaaag aagcaggttc cctgttttgc ctcaatgcta actaaaaagc
      361 tgcatttctt tccaaaatct aatctcgagc accaccacca ccaccactga gatccggctg
      421 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      481 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      541 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      601 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      781 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      841 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      901 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      961 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     1021 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1081 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1141 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1201 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1261 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1321 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1381 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1441 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1501 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1561 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1621 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1681 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1741 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1801 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1861 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1921 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1981 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2041 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2281 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2881 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2941 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
     3001 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     3061 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3121 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3181 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     3241 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     3301 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     3361 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     3421 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     3481 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     3541 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     3601 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     3661 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     3721 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     3781 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     3841 cacccgtggc caggacccaa cgctgcccga gatctcgatc ccgcgaaat
//
