LOCUS       sequence-20102-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  TITLE     Johnson lab plasmids transferred from original pMEX to pcDNA
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA 3.1(-) rat C/EBP beta (LIP)
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      148..157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(621..638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctnctagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc
      121 actgtgctgg atatctgcag aattcgcgcc accatggcgg ccggcttccc gttcgccctg
      181 cgcgcctacc tgggctacca ggcgacgccg agcggcagca gcggcagcct gtccacgtcg
      241 tcgtcgtcca gcccgcccgg gacgccgagc cccgccgacg ccaaggccgc gcccgccgcc
      301 tgcttcgcgg ggccgccggc cgcgcccgcc aaggccaagg ccaagaaggc ggtggacaag
      361 ctgagcgacg agtacaagat gcggcgcgag cgcaacaaca tcgcggtgcg caagagccgc
      421 gacaaggcca agatgcgcaa cctggagacg cagcacaagg tgctggagct gacggcggag
      481 aacgagcggc tgcagaagaa ggtggagcag ctgtcgcgag agctcagcac gctgcggaac
      541 ttgttcaagc agctgcccga gccgctgctg gcctcggccg gtcactgcta gcccaagctt
      601 aagtttaaac cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      661 cccctccccc gtgccttcct tgaccctgga aggtgccact
//
