LOCUS       sequence-201057-        4568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty AAV vector with TBG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4568)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV.TBG.PI.Null.bGH
FEATURES             Location/Qualifiers
     source          1..4568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        470..879
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin 
                     gene (Yan et al., 2012)"
     intron          939..1071
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1054..1073
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(1327..1344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1333..1540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1628..1757
                     /label=AAV2 ITR
     primer_bind     complement(1776..1793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1776..1792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1785..1807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1934..2389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2021..2040)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2231..2252
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2416..2520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2521..2589,2590..3381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVVMVTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2739..2758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3552..4140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4041..4060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4428..4449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4464..4469,4470..4487,4488..4494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4502..4518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4507..4529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4526..4542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4526..4542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc aggttaattt ttaaaaagca gtcaaaagtc
      241 caagtggccc ttggcagcat ttactctctc tgtttgctct ggttaataat ctcaggagca
      301 caaacattcc agatccaggt taatttttaa aaagcagtca aaagtccaag tggcccttgg
      361 cagcatttac tctctctgtt tgctctggtt aataatctca ggagcacaaa cattccagat
      421 ccggcgcgcc agggctggaa gctacctttg acatcatttc ctctgcgaat gcatgtataa
      481 tttctacaga acctattaga aaggatcacc cagcctctgc ttttgtacaa ctttccctta
      541 aaaaactgcc aattccactg ctgtttggcc caatagtgag aactttttcc tgctgcctct
      601 tggtgctttt gcctatggcc cctattctgc ctgctgaaga cactcttgcc agcatggact
      661 taaacccctc cagctctgac aatcctcttt ctcttttgtt ttacatgaag ggtctggcag
      721 ccaaagcaat cactcaaagt tcaaacctta tcattttttg ctttgttcct cttggccttg
      781 gttttgtaca tcagctttga aaataccatc ccagggttaa tgctggggtt aatttataac
      841 taagagtgct ctagttttgc aatacaggac atgctataaa aatggaaaga tgttgctttc
      901 tgagagactg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
      961 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
     1021 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccagg
     1081 cggccaattc gaatggccat gggacgtcga cctgaggtaa ttataacccg ggccctatat
     1141 atggatccaa ttgcaatgat catcatgaca gatctgcgcg cgatcgattg gccacgtggg
     1201 ccgtgcacct taagcttatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg
     1261 cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataag
     1321 gatctgcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     1381 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     1441 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     1501 agggggagga ttgggaagac aatagcaggc atgctgggga ctcgagttaa gggcgaattc
     1561 ccgattagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     1621 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     1681 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     1741 agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt
     1801 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     1861 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     1921 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1981 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2041 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2101 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2161 tcacgtagtg ggccatcgcc ccgatagacg gtttttcgcc ctttgacgct ggagttcacg
     2221 ttcctcaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     2281 tcttttgatt tataagggat ttttccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2341 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttataatttc aggtggcatc
     2401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2641 cgagtgggtt acatcgaact ggatctcaat agtggtaaga tccttgagag ttttcgcccc
     2701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2761 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3061 cctgtagtaa tggtaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3661 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3961 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctgcggttt tgctcacatg
     4201 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4261 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4321 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4381 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4441 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4501 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
     4561 aattaagg
//
