LOCUS       sequence-20106-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Bateman JR, Lee AM, Wu CT
  TITLE     Site-specific transformation of Drosophila via phiC31 
            integrase-mediated cassette exchange.
  JOURNAL   Genetics. 2006 Jun . 173(2):769-77.
  PUBMED    16547094
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2006 Jun . 173(2):769-77."
COMMENT     Sequence Label: pUASTP2
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..193
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        212..450
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     397..418
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    546..645
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 ttttaaagtt ttatttttaa taatttgcga gtacgcaaag cttggctgca tccaacgcgt
       61 tgggagctct ccggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
      121 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      181 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
      241 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
      301 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
      361 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
      421 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcg gcttcgacta
      481 gtactgacgg acacaccgaa gccccggcgg caaccctcag cggatgcccc ggggcttcac
      541 gttttcccag gtcagaagcg gttttcggga gtagtgcccc aactggggta acctttgagt
      601 tctctcagtt gggggcgtag ggtcgccgac atgacacaag gggttgtgac cggggtggac
      661 acgtacgcgg gtgct
//
