LOCUS       sequence-201065-        6018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated 
            tdtomato for tracing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ,
            Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, 
            Gradinaru V
  TITLE     Systemic AAV vectors for widespread and targeted gene delivery in 
            rodents.
  JOURNAL   Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.
  PUBMED    30626963
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Protoc"; date: "2019-02"; volume: "14"; issue: "2"; pages: 
            "379-414"
COMMENT     Sequence Label: pAAV-hSyn1-tdTomatof
FEATURES             Location/Qualifiers
     source          1..6018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..468
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1991..2579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2044..2064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2462..2473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2611..3087
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2810..2829)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3127..3267
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3127..3256
                     /label=AAV2 ITR
     rep_origin      3342..3797
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3429..3448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3639..3660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3814..3833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3933..3955
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3993..4011)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4079..4183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4184..4252,4253..5044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4402..4421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5215..5803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5704..5723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5865..5994
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
       61 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
      121 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
      181 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
      241 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
      301 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
      361 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
      421 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaggtac
      481 cgccaccatg gtgtcaaagg gagaggaagt catcaaggaa ttcatgcggt ttaaagttcg
      541 catggaaggg agcatgaacg gccatgaatt cgagatcgaa ggggagggag agggccgacc
      601 atacgagggg acacagacgg cgaaattgaa agtgaccaaa ggaggaccct tgccattcgc
      661 ttgggacatt ctgtcccctc agttcatgta cggaagtaag gcctatgtga agcatcctgc
      721 cgatattccc gactataaga aactgtcctt cccggagggg ttcaagtggg aaagagtgat
      781 gaatttcgag gatggcgggc tcgtgaccgt cacccaagat agcagcttgc aggacggaac
      841 actgatctac aaggtcaaga tgaggggcac taatttccct cctgatggtc ctgtcatgca
      901 aaagaaaacc atggggtggg aagcaagtac ggaacgcctg tacccacggg acggtgttct
      961 gaagggcgag atacatcagg cacttaaact caaggacggc ggccactatc tggtcgaatt
     1021 taagacgatc tatatggcaa agaaaccagt acagctcccg ggttactact atgtggacac
     1081 taaacttgac attacaagtc acaacgagga ctataccatc gtggaacagt atgaacgctc
     1141 tgaggggagg caccatctgt ttcttggcca tggaacaggc agcacaggat ctggatcatc
     1201 tggcactgca tccagcgagg ataacaacat ggccgtgatt aaggagttca tgaggttcaa
     1261 agttcgtatg gagggatcaa tgaatggcca cgaattcgaa atcgagggtg aaggggaggg
     1321 tagaccctac gagggcactc agacagccaa gctgaaggta accaagggcg gacctctgcc
     1381 ttttgcttgg gacatcctgt ctccccagtt tatgtacggt agcaaggcct atgtcaaaca
     1441 cccagctgac atcccggact acaagaagct ctcctttcca gaaggcttta agtgggagcg
     1501 ggttatgaac ttcgaggatg gtgggcttgt aactgtgacc caggattcca gtctgcagga
     1561 tgggactctg atttacaaag tgaaaatgcg tggaactaac tttccacccg atggccccgt
     1621 tatgcagaag aaaacaatgg gctgggaagc tagcaccgag agactgtatc ctagggatgg
     1681 agtgctgaag ggtgagatcc accaagctct gaaactcaaa gatggcggac attacctggt
     1741 ggagtttaag accatataca tggccaagaa gcccgtgcaa ttgcccgggt actactacgt
     1801 agacacaaag ctggacataa cctcacacaa tgaggactac acaattgtcg aacagtatga
     1861 gcgatctgag ggtagacacc acctctttct ctatgggatg gatgagctgt ataaaaagct
     1921 gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc tctcctgaaa
     1981 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2041 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2101 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2161 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2221 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2281 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2341 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2401 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2461 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2521 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     2581 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     2641 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     2701 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     2761 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     2821 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     2881 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     2941 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     3001 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     3061 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc
     3121 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     3181 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     3241 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     3301 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     3361 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     3421 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3481 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3541 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3601 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3661 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     3721 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3781 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3841 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3901 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3961 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4021 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4081 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4141 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4201 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4261 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4321 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4381 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4441 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4501 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4561 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4621 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4681 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4741 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4801 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4861 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4921 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4981 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5041 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5101 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5341 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcagctgcg
     5881 cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag
     5941 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tcctgcggcc
     6001 gcacgcgtgt gtctagac
//
