LOCUS       sequence-20107-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Bateman JR, Lee AM, Wu CT
  TITLE     Site-specific transformation of Drosophila via phiC31 
            integrase-mediated cassette exchange.
  JOURNAL   Genetics. 2006 Jun . 173(2):769-77.
  PUBMED    16547094
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2006 Jun . 173(2):769-77."
COMMENT     Sequence Label: piB-GFP
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..83
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     273..289
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     274..290
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        342..580
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     527..548
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
       61 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
      121 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
      181 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
      241 tcagcgggtt ctcgacggtc acggcgggca tgtcgaggtc gacggtatcg ataagcttga
      301 tatcgaattc ctgcagcccg ggggatccgg cgaaaagagc gccggagtat aaatagaggc
      361 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
      421 aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag
      481 ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat
      541 ctgccaagaa gtaattattg aatacaagaa gagaactctg aatactttca acaagttacc
      601 gagaaagaag aactcacaca caatgcctac tagtaagggc gaggaactgt tcactggcgt
      661 ggtcccaatt ctcgtggaac tggatggcga tgtga
//
