LOCUS       sequence-201072-        7465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of Cre from GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7465)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV.GFAP.Cre.WPRE.hGH
FEATURES             Location/Qualifiers
     source          1..7465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     misc_feature    146..263
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2536..3567
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    3581..4169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3634..3654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4052..4063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4201..4677
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4400..4419)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4717..4857
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4717..4846
                     /label=AAV2 ITR
     rep_origin      4932..5387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5019..5038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5229..5250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5404..5423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5523..5545
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5583..5601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5669..5773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5774..5842,5843..6634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6805..7393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7294..7313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(7455..7465,1..130)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttttta aaagaaaagg ggggattggg gggtacagtg
      181 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
      241 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
      301 taattaaccc acctccctct ctgtgctggg actcacagag ggagacctca ggaggcagtc
      361 tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg cgcacaactg
      421 taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga agttctagac
      481 cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa tcagccacgt
      541 gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg gatcacttaa
      601 ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca gcctgggcaa
      661 cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa catatcctgg
      721 tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc tctgtgagct
      781 ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat tgggctggcc
      841 gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt
      901 tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga
      961 gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg catcttggga
     1021 taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga
     1081 acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca ggcatggaca
     1141 gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag gacacaaatg
     1201 ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc gtagatggac
     1261 ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc ccagtggctc
     1321 cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg ctgatgtgtg
     1381 ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga aacggggcgc
     1441 tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg tggagctgtc
     1501 aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg tgacctggag
     1561 ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc ctcttgccct
     1621 atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag ggctggtgtc
     1681 caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc aggaatgtgg
     1741 gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc tagtaggaaa
     1801 tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc agcaccttgc
     1861 aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt tggaaagcag
     1921 gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt ttgtgagaca
     1981 aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc agcctcaacc
     2041 tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt acaagcatga
     2101 gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg taagtattca
     2161 tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc tcttgcttca
     2221 gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca tgcaggagtt
     2281 ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga ggggagctct
     2341 ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca
     2401 ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc atcccaggag
     2461 cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc gccgggatcg
     2521 cgggaattcg ccaccatgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
     2581 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
     2641 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
     2701 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
     2761 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
     2821 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
     2881 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
     2941 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
     3001 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
     3061 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     3121 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     3181 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     3241 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     3301 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     3361 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
     3421 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
     3481 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
     3541 gtgcgcctgc tggaagatgg cgattagcca gcttatcgat aatcaacctc tggattacaa
     3601 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3661 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3721 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3781 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3841 ctgtcagctc ctttccggaa ctttcgcttt ccccctccct attgccacgg cggaactcat
     3901 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3961 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat
     4021 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4081 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4141 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     4201 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     4261 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     4321 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     4381 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     4441 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     4501 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     4561 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     4621 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     4681 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     4741 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4801 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4861 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4921 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4981 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5041 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5101 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     5161 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5221 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     5281 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5341 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     5401 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5461 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5521 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5581 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5641 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5701 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5761 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5821 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5881 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5941 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6001 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6061 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6121 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6181 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6241 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6301 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6361 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6421 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6481 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6541 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6601 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6661 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6721 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6781 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6841 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6901 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6961 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7021 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7081 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7141 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7201 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7261 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7321 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7381 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7441 ttttgctcac atgtcctgca ggcag
//
