LOCUS       sequence-201074-        5196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of TurboRFP from hSyn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5196)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENN.AAV.hSyn.TurboRFP.WPRE.RBG
FEATURES             Location/Qualifiers
     source          1..5196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        228..675
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             701..1396
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     misc_feature    1406..1994
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1459..1479)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1877..1888)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2033..2052)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2098..2153
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   2246..2375
                     /label=AAV2 ITR
     primer_bind     complement(2394..2411)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2394..2410)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2403..2425)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2552..3007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2639..2658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2849..2870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3033..3137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3138..3206,3207..3998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3356..3375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4169..4757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4658..4677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5045..5066
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5081..5086,5087..5104,5105..5111)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5119..5135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5124..5146
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5143..5159
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5143..5159
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg
      181 atcctctaga actatagcta gcatgcctgc agagggccct gcgtatgagt gcaagtgggt
      241 tttaggacca ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg
      301 gacaagcacc caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga
      361 aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg
      421 cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc
      481 gccggtcccc cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc
      541 ccagccggac cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc
      601 tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg
      661 tgcctgagag cgcagtcgaa ttcaagctgc tagtgccacc atgagcgagc tgatcaagga
      721 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
      781 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgaagatca aggtggtcga
      841 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
      901 agccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg
      961 cttcacatgg gagagaatca ccacatacga agacgggggc gtgctgaccg ctacccagga
     1021 caccagcttc cagaacggct gcatcatcta caacgtcaag atcaacgggg tgaacttccc
     1081 atccaacggc cctgtgatgc agaagaaaac acgcggctgg gaggccaaca ccgagatgct
     1141 gtaccccgct gacggcggcc tgagaggcca cagccagatg gccctgaagc tcgtgggcgg
     1201 gggctacctg cactgctcct tcaagaccac atacagatcc aagaaacccg ctaagaacct
     1261 caagatgccc ggcttccact tcgtggacca cagactggaa agaatcaagg aggccgacaa
     1321 agagacctac gtcgagcagc acgagatggc tgtggccaag tactgcgacc tccctagcaa
     1381 actggggcac agatgactcg acgataatca acctctggat tacaaaattt gtgaaagatt
     1441 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     1501 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     1561 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     1621 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     1681 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     1741 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     1801 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     1861 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     1921 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     1981 ggccgcctcc ccgcatcgtc gaggacgggg tgaactacgc ctgaggatcc gatctttttc
     2041 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     2101 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     2161 gcaattcgtt gatctgaatt tcgaccaccc ataataccca ttaccctggt agataagtag
     2221 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     2281 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     2341 gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc
     2401 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2461 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2521 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     2581 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2641 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2701 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2761 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2821 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2881 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2941 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3001 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3061 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4861 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4921 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4981 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5041 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5101 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5161 atgattacgc cagatttaat taaggcctta attagg
//
