LOCUS       sequence-201075-        5616 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  AAV expression of Cre from hSyn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5616)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENN.AAV.hSyn.Cre.WPRE.hGH
FEATURES             Location/Qualifiers
     source          1..5616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        225..672
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             687..1718
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    1732..2320
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1785..1805)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2203..2214)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2352..2828
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2551..2570)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2868..3008
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2868..2997
                     /label=AAV2 ITR
     rep_origin      3083..3538
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3170..3189)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3380..3401
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3555..3574)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3674..3696
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3734..3752)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3820..3924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3925..3993,3994..4785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4143..4162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4956..5544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5445..5464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5606..5616,1..130)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttttta aaagaaaagg ggggattggg gggtacagtg
      181 caggggaaag aatagtagga tctctgcaga gggccctgcg tatgagtgca agtgggtttt
      241 aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
      301 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
      361 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
      421 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
      481 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
      541 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
      601 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
      661 ctgagagcgc agtcgaattc gccaccatgt ccaatttact gaccgtacac caaaatttgc
      721 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca
      781 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
      841 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc
      901 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt
      961 tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg
     1021 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa
     1081 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata
     1141 gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt
     1201 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga
     1261 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg
     1321 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg
     1381 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg
     1441 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga
     1501 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
     1561 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
     1621 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
     1681 caggggcaat ggtgcgcctg ctggaagatg gcgattagcc agcttatcga taatcaacct
     1741 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1801 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1861 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1921 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1981 attgccacca cctgtcagct cctttccgga actttcgctt tccccctccc tattgccacg
     2041 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2101 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt
     2161 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2221 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2281 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc
     2341 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     2401 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     2461 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2521 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2581 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     2641 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     2701 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     2761 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     2821 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     2881 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     2941 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3001 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3061 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3121 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3181 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3241 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     3301 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3361 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3421 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3481 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     3541 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3601 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3661 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3721 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3781 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3901 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4141 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4441 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4801 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4861 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5101 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5581 ttttgctggc cttttgctca catgtcctgc aggcag
//
