LOCUS       sequence-201091-        3639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides Glycine max P1 matrix attachment region as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3639)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD1:P1-MAR
FEATURES             Location/Qualifiers
     source          1..3639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(686..704)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(687..704)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(722..738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(722..738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(735..757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    746..762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(770..776,777..794,795..800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    815..836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(953..970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1124..1712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1204..1223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1883..2674,2675..2743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2506..2525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2744..2848)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2926..3381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3063..3084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3275..3294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3507..3529
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3521..3538
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3522..3538
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3545..3564
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3545..3563
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3594..3603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3598..3607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggaggtgaat tctaaactta tattgagata ttagtgtata atataatttc cgcactctct
       61 tttaaattaa taatacaaga tttagaaaaa atgaacttta attttgagat attagtgtgt
      121 aattctcagt agagaatttc ctaagttcac ccaaaagtat atcattttcc tcttaagaaa
      181 atacaaacac tacctaattt tatcccctat aaatatctaa aaatttgcat ctcataaaat
      241 ttaccaatta tttatttttt aagatatttt actaattatc tataactatt aataatcaaa
      301 attatttcat tgatgtacat atttcaatag ataatttacc ccttaatcac ttaataaatt
      361 ttaaattttc attattttta tataatttat agtctttttt attaactata tttaaatttt
      421 attttttatt attaaaaaaa tttagagaga cacatttttc cctaattagt catatataag
      481 aaaaataaca tttgggtaaa atgtgagagc ccaaacgcaa ttcgtgttgg gcctaaaggg
      541 cccacgaagt agatactaaa ggatgccctc atcgatgaat gcttcgctcg agaccactca
      601 tcgcccatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa
      661 cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat
      721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1981 gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg
     2041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgct ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 attgtaatac gactcactat agggcgaatt gggcccgacg tcgcatgctc ccggccgcca
     3601 tggcggccgc gggaattcga tgggcgatga gtggtctcg
//
