LOCUS       sequence-201103-       13935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, 
            Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch
            R, Bradner JE, Gray NS, Young RA
  TITLE     YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
  JOURNAL   Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 
            10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
  PUBMED    29224777
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.11.008"; journalName: "Cell"; date: 
            "2017-12-14- 14"; volume: "171"; issue: "7"; pages: "1573-1588.e28"
COMMENT     Sequence Label: pAW91.dCas9
FEATURES             Location/Qualifiers
     source          1..13935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(37..56)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        228..607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        609..807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     762..782
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             825..1005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1052..1177
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1670..1903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2088..2132
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2430..2547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2677..3855
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2908..3846
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3803..3823
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3871..3918
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(3919..3942,3943..3945,3946..6501,6502..6504,
                     6505..8019)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8074..8094
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8140..8193
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8194..8592
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8617..9205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8670..8690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9088..9099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9208..9224)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9209..9225)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9730..9910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9936..9953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9942..10166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10212..10640
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10299..10318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10509..10530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10649..10669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10654..10983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10834..10969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10896..10915
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11031..11078
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11097..11471
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11601..11722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11638..11657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11692..11711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11771..11787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11771..11787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11784..11806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11795..11811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11819..11825,11826..11843,11844..11849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11864..11885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12002..12019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12173..12761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12253..12272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12932..13723,13724..13792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13555..13574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13793..13897)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
       61 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      121 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      181 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      241 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      361 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      421 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      481 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      541 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      601 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      661 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      721 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      781 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      841 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      901 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      961 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1021 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1081 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1141 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1201 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1261 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1321 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1381 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1441 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1501 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1561 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1621 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1681 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1741 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1801 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1861 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1921 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1981 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     2041 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2101 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2161 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2221 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2281 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2341 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2401 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2461 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2521 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2581 tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     2641 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     2701 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     2761 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     2821 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     2881 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     2941 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     3001 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     3061 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     3121 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     3181 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     3241 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     3301 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     3361 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     3421 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     3481 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3541 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     3601 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     3661 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     3721 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     3781 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     3841 tttcaggtgt cgtgacgtac ggccaccatg aaaaggccgg cggccacgaa aaaggccggc
     3901 caggcaaaaa agaaaaagga caagaagtac agcatcggcc tggccatcgg caccaactct
     3961 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     4021 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
     4081 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     4141 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     4201 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     4261 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     4321 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     4381 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     4441 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     4501 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     4561 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     4621 ggcctgttcg gcaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     4681 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     4741 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     4801 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     4861 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     4921 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     4981 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     5041 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
     5101 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     5161 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     5221 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     5281 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     5341 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     5401 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     5461 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     5521 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     5581 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     5641 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     5701 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     5761 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     5821 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     5881 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     5941 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     6001 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     6061 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     6121 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     6181 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     6241 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     6301 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     6361 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     6421 tacgatgtgg accacatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     6481 gtgctgacca gaagcgacaa ggcccggggc aagagcgaca acgtgccctc cgaagaggtc
     6541 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     6601 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     6661 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     6721 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     6781 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     6841 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     6901 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     6961 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     7021 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     7081 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     7141 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     7201 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     7261 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     7321 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     7381 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     7441 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     7501 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     7561 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     7621 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     7681 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
     7741 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
     7801 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
     7861 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
     7921 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     7981 tacgagacac ggatcgacct gtctcagctg ggaggcgaca gcgctggagg aggtggaagc
     8041 ggaggaggag gaagcggagg aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc
     8101 gctggatccg gacgggctat taactgtaca ggcagtggag agggcagagg aagtctgcta
     8161 acatgcggtg acgtcgagga gaatcctggc ccaatggcca agcctttgtc tcaagaagaa
     8221 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     8281 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     8341 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     8401 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     8461 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     8521 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     8581 tgggagggct aagaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt
     8641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     9001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     9061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     9121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     9181 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     9241 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     9301 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     9361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     9421 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     9481 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     9541 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     9601 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     9661 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     9721 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9901 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     9961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
    10021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
    10081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
    10141 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
    10201 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    10261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    10321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
    10381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    10441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
    10501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
    10561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
    10621 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
    10681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
    10741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    10861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10921 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    10981 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
    11041 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
    11101 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
    11161 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
    11221 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    11281 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    11341 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    11401 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    11461 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
    11521 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    11581 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    11641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11701 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    11761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    11821 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    11881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    11941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    12001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    12181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    12241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    12301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    12361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    12421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    12481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    12541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    12601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    12661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    12721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    12781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    12841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    12901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    12961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    13021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    13081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    13141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    13201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    13261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    13321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    13381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    13441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    13501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    13561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    13621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    13681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    13741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    13801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    13861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    13921 ctgacgtcga cggat
//
