LOCUS       sequence-201124-        9533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TN-DROSHA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Chang TC, Pertea M, Lee S, Salzberg SL, Mendell JT
  TITLE     Genome-wide annotation of microRNA primary transcript structures 
            reveals novel regulatory mechanisms.
  JOURNAL   Genome Res. 2015 Sep;25(9):1401-9. doi: 10.1101/gr.193607.115.
  PUBMED    26290535
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gr.193607";
            journalName: "Genome Res"; date: "2015-09"; volume: "25"; issue: 
            "9"; pages: "1401-9"
COMMENT     Sequence Label: pcDNA3.1/V5-His-TN-DROSHA
FEATURES             Location/Qualifiers
     source          1..9533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5030..5071
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5081..5098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5121..5138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5127..5351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5397..5825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5484..5503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5694..5715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5834..5854)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5839..6169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6020..6155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6082..6101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6236..7030
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6290..6309)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6900..6919
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7206..7327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7243..7262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7297..7316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7376..7392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7376..7392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7389..7411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7400..7416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7424..7430,7431..7448,7449..7454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7469..7490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7607..7624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7778..8366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7858..7877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8537..9328,9329..9397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9160..9179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9398..9502)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaaagg gtcaagacaa ttctgcagat
      961 caccatggca atgatgcagg gaaacacatg tcacagaatg tcgttccacc cgggacgagg
     1021 gtgtccccga ggacgaggag gacatggagc cagaccctca gcaccatcct ttaggcccca
     1081 aaatctgagg ctgcttcacc ctcagcagcc tcctgtgcaa tatcaatatg aacctccaag
     1141 tgccccttcc accactttct caaactctcc agcccccaat tttctccctc cacgaccaga
     1201 ctttgtaccc ttccccccac ccatgcctcc gtcagcgcaa ggccctcttc ccccctgccc
     1261 aatcaggccg cctttcccca accaccagat gaggcacccc ttcccagttc ctccttgttt
     1321 tcctcccatg ccaccaccaa tgccttgtcc taataacccc ccagtccctg gggcacctcc
     1381 tggacaaggc actttcccct tcatgatgcc ccctccctcc atgcctcatc ccccgccccc
     1441 tccagtcatg ccgcagcagg ttaattatca gtaccctccg ggctattctc accacaactt
     1501 cccacctccc agttttaata gtttccagaa caaccctagt tctttcctgc ccagtgctaa
     1561 taacagcagt agtcctcatt tcagacatct ccctccatac ccactcccaa aggctcccag
     1621 tgagagaagg tccccagaaa ggctgaaaca ctatgatgac cacaggcacc gagatcacag
     1681 tcatgggcga ggtgagaggc atcggtccct ggatcggcgg gagcgaggcc gcagtcccga
     1741 caggagaaga caagacagcc ggtacagatc tgattatgac cgagggagaa caccatctcg
     1801 ccaccgcagc tacgaacgga gcagagagcg agaacgggag agacacaggc atcgagacaa
     1861 ccgaagatca ccatctctgg aaaggtccta caaaaaagag tataagagat ctggaagtcg
     1921 ctccccaagt agggagaaga agagagctcg ttgggaggaa gaaaaagacc gttggagtga
     1981 caaccagagt tctggcaaag acaagaacta tacctcaatc aaggaaaaag agcccgagga
     2041 gaccatgcct gacaagaatg aggaggaaga agaagaactt cttaagcctg tgtggattcg
     2101 atgcactcat tcagaaaact actactccag tgaccccatg gatcaggtgg gagattctac
     2161 agtggttgga acgagtaggc ttcgtgactt atatgacaaa tttgaggagg agttggggag
     2221 caggcaagaa aaggccaaag ctgctcggcc tccgtgggaa cctccaaaga cgaagctcga
     2281 tgaagattta gagagttcca gtgaatccga gtgtgagtct gatgaggaca gcacctgttc
     2341 tagcagctca gactctgaag tttttgacgt tattgcagaa atcaaacgca aaaaggccca
     2401 ccctgaccga cttcatgatg aactttggta caacgatcca ggccagatga atgatggacc
     2461 actctgcaaa tgcagcgcaa aggcaagacg cacaggaatt aggcacagca tttatcctgg
     2521 agaagaggcc atcaagccct gtcgtcctat gaccaacaat gctggcagac ttttccacta
     2581 ccggatcaca gtctccccgc ctacgaactt tttaactgac aggccaactg ttatagaata
     2641 cgatgatcac gagtatatct ttgaaggatt ttctatgttt gcacatgccc ccctgaccaa
     2701 tattccactg tgtaaagtaa ttagattcaa catagactac acgattcatt tcattgaaga
     2761 gatgatgccg gagaattttt gtgtgaaagg gcttgaactc ttttcactgt tcctattcag
     2821 agatattttg gaattatatg actggaatct taaaggtcct ttgtttgaag acagccctcc
     2881 ctgctgccca agatttcatt tcatgccacg ttttgtaaga tttcttccag atggaggaaa
     2941 ggaagtgctg tccatgcacc agattctcct gtacttgtta aggtgcagca aagccctggt
     3001 gcctgaggag gagattgcca atatgcttca gtgggaggag ctggagtggc agaaatatgc
     3061 agaagaatgc aaaggcatga ttgttaccaa ccctgggacg aaaccaagct ctgtccgtat
     3121 cgatcaactg gatcgtgaac agttcaaccc cgatgtgatt acttttccga ttatcgtcca
     3181 ctttgggata cgccctgcac agttgagtta tgcaggagac ccacagtacc aaaaactgtg
     3241 gaagagttat gtgaaacttc gccacctcct agcaaatagt cccaaagtca aacaaactga
     3301 caaacagaag ctggcacaga gggaggaagc cctccaaaaa atacggcaga agaatacaat
     3361 gagacgagaa gtaacggtgg agctaagtag ccaaggattc tggaaaactg gcatccgttc
     3421 tgatgtctgt cagcatgcaa tgatgctacc tgttctgacc catcatatcc gctaccacca
     3481 atgcctaatg catttggaca agttgatagg atatactttc caagatcgtt gtctgttgca
     3541 gctggccatg actcatccaa gtcatcattt aaattttgga atgaatcctg atcatgccag
     3601 gaattcatta tctaactgtg gaattcggca gcccaaatac ggagacagaa aagttcatca
     3661 catgcacatg cggaagaaag ggattaacac cttgataaat atcatgtcac gccttggcca
     3721 agatgaccca actccctcga ggattaacca caatgaacgg ttggaattcc tgggtgatgc
     3781 tgttgttgaa tttctgacca gcgtccattt gtactatttg tttcctagtc tggaagaagg
     3841 aggattagca acctatcgga ctgccattgt tcagaatcag caccttgcca tgctagcaaa
     3901 gaaacttgaa ctggatcgat ttatgctgta tgctcacggg cctgaccttt gtagagaatc
     3961 ggaccttcga catgcaatgg ccaattgttt tcaagcgtta ataggagctg tttacttgga
     4021 gggaagcctg gaggaagcca agcagttatt tggacgcttg ctctttaatg atccggacct
     4081 gcgcgaagtc tggctcaatt atcctctcca cccactccaa ctacaagagc caaatactga
     4141 tcgacaactt attgaaactt ctccggttct acaaaaactt actgagtttg aagaagcaat
     4201 tggagtaatt tttactcatg ttcgacttct ggcaagggca ttcacattga gaactgtggg
     4261 atttaaccat ctgaccctag gccacaatca gagaatggaa ttcctaggtg actccataat
     4321 gcaactggta gccacagagt acttattcat tcatttccca gatcatcatg aaggacactt
     4381 aactttgttg cgaagctctt tggtgaataa tagaactcag gccaaggtag cggaggagct
     4441 gggcatgcag gagtacgcca taaccaacga caagaccaag aggcctgtgg cgcttcgcac
     4501 caagaccttg gcggaccttt tgcaatcatt tattgcagcg ctgtacattg ataaggattt
     4561 ggaatatgtt catactttca tgaatgtctg cttctttcca cgattgaaag agttcatttt
     4621 gaatcaggat tggaatgacc ccaaatccca gcttcagcag tgttgcttga cacttaggac
     4681 agaaggaaaa gagccagaca ttcctctgta caagactctg cagacagtgg gcccatccca
     4741 tgcccgaacc tacactgtgg ctgtttattt caagggagaa agaataggct gtgggaaagg
     4801 accaagtatt cagcaagcgg aaatgggagc agcaatggat gcgcttgaaa aatataattt
     4861 tccccagatg gcccatcaga agcggttcat cgaacggaag tacagacaag agttaaaaga
     4921 aatgaggtgg gaaagagagc atcaagagag agagccagat gagactgaag acatcaagaa
     4981 aatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5041 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     5101 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5161 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5221 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5281 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5341 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5521 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc
     5581 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5641 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5701 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5761 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5821 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc
     5881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6181 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6241 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6301 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6361 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6421 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6481 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6541 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6601 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6661 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6721 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6781 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6841 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6901 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6961 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7021 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     7081 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     7141 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     7201 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7321 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7381 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7441 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7621 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7681 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     7981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
