LOCUS       sequence-201126-        2828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus YFP fused with silaffin-R5 silica affinity peptide 
            under the control of TetO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Olmez TT, Yuca E, Eyupoglu E, Catalak HB, Sahin O, Seker UOS
  TITLE     Autonomous Synthesis of Fluorescent Silica Biodots Using Engineered 
            Fusion Proteins.
  JOURNAL   ACS Omega. 2018 Jan 31;3(1):585-594. doi: 10.1021/acsomega.7b01769. 
            Epub 2018 Jan 18.
  PUBMED    30023783
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.7b01769"; journalName: "ACS Omega"; date: 
            "2018-01-31- 31"; volume: "3"; issue: "1"; pages: "585-594"
COMMENT     Sequence Label: pZA-YFP-R5
FEATURES             Location/Qualifiers
     source          1..2828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(660..754)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(775..1434)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1349..1368
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1435..1537)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1603..1621
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1642..1660,1661..1666,1667..1683,1684..1689,
                     1690..1715)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1642..1660
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1667..1685
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1725..1736
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2573..2659
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2695..2712)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
      121 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      181 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      241 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      301 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
      361 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
      421 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
      481 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
      541 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
      601 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttact agtgcttgga
      661 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
      721 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
      781 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      841 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      901 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      961 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1021 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1081 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1141 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1201 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1261 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1321 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1381 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1441 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1501 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccagatatc
     1561 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     1621 ccctttcgtc ttcacctcga gtccctatca gtgatagaga ttgacatccc tatcagtgat
     1681 agagatactg agcacatcag caggacgcac tgaccgaatt cattaaagag gagaaaggta
     1741 ccatgagcaa aggtgaagaa ctgttcaccg gcgttgtgcc aattctggtt gagctggatg
     1801 gtgacgtgaa tggccacaaa ttttccgtgt ctggtgaagg cgagggtgat gctacttatg
     1861 gcaaactgac tctgaaactg atctgtacca ccggcaaact gcctgttccg tggccaactc
     1921 tggtcactac tctgggttac ggcctgatgt gttttgcgcg ttacccggat cacatgaaac
     1981 agcatgactt cttcaaatct gccatgccgg aaggctatgt ccaagaacgt acgatctttt
     2041 tcaaggacga cggcaactat aaaacccgtg ccgaagttaa attcgagggt gacaccctgg
     2101 ttaaccgcat cgaactgaaa ggcattgact tcaaagagga cggcaacatt ctgggtcaca
     2161 agctggaata caactacaac tcccacaacg tttacattac tgctgacaag cagaaaaacg
     2221 gcatcaaagc aaacttcaag atccgtcaca acattgaaga tggtggcgta cagctggcag
     2281 atcactacca gcagaacact ccaatcggtg atggcccagt actgctgcca gataaccatt
     2341 acctgtccta ccagagcaaa ctgtctaaag acccgaacga aaaacgtgac cacatggtac
     2401 tgctggaatt tgttaccgcg gcaggcatta cccacggtat ggacgaactg tataaaggtg
     2461 gcggtggcag tggcggcggc ggtagcggcg ggggaggatc tagcagcaaa aaaagcggca
     2521 gctatagcgg cagcaaaggc agcaaacgcc gcattctgta aacgcgtggc atcaaataaa
     2581 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2641 tctcctgagt aggacaaatc cgccgcccta gacctagggg atatattccg cttcctcgct
     2701 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     2761 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     2821 aagccgtt
//
