LOCUS       sequence-201144-       10887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector expressing plant-codon-optimized Cas9 under UBQ10
            promoter, with sgRNAs be shuffled in; seed coat specific red 
            fluorescence for screening trangene free plants;.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, 
            Schwab R, Weigel D
  TITLE     An efficient CRISPR vector toolbox for engineering large deletions 
            in Arabidopsis thaliana.
  JOURNAL   Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. 
            eCollection 2018.
  PUBMED    30083222
REFERENCE   2  (bases 1 to 10887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-08-2"; volume: "14:65. doi"; pages: " 
            10.1186/s13007-018-0330-7. eCollection 2018"
COMMENT     Sequence Label: pRW004-SM-destination-proUBQ10-Cas9
FEATURES             Location/Qualifiers
     source          1..10887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             1232..1279
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1286..1306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1331..2767,2957..5620)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2768..2956
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5621..5668
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5674..6315
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(6538..6861)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     6771..6787
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6857..6873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6857..6873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6870..6892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6881..6897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6905..6911,6912..6929,6930..6935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6950..6971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7088..7105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7484..7736)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(8020..8039)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8289..8307
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8906..8930
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9021..9609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9101..9120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9780..10568)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(10859..10887,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagccga cgagtcagta
      601 ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc
      661 acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa
      721 acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct
      781 cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc
      841 tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc
      901 tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg
      961 ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc
     1021 gaagacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat
     1081 agatatcatc cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg
     1141 tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtagattaat
     1201 ctgagttttt ctgattaaca gcggatccat ggattacaag gatgatgatg ataaggatta
     1261 caaggatgat gatgataaga tggctccaaa gaagaagaga aaggttggaa tccacggagt
     1321 tccagctgct gataagaagt actctatcgg acttgacatc ggaaccaact ctgttggatg
     1381 ggctgttatc accgatgagt acaaggttcc atctaagaag ttcaaggttc ttggaaacac
     1441 cgatagacac tctatcaaga agaaccttat cggtgctctt cttttcgatt ctggagaaac
     1501 cgctgaggct accagattga agagaaccgc tagaagaaga tacaccagaa gaaagaacag
     1561 aatctgctac cttcaggaaa tcttctctaa cgagatggct aaggttgatg attctttctt
     1621 ccacagactt gaggagtctt tccttgttga ggaggataag aagcacgaga gacacccaat
     1681 cttcggaaac atcgttgatg aggttgctta ccacgagaag tacccaacca tctaccacct
     1741 tagaaagaag ttggttgatt ctaccgataa ggctgatctt agacttatct accttgctct
     1801 tgctcacatg atcaagttca gaggacactt ccttatcgag ggtgacctta acccagataa
     1861 ctctgatgtt gataagttgt tcatccagct tgttcagacc tacaaccagc ttttcgagga
     1921 gaacccaatc aacgcttctg gagttgatgc taaggctatc ctttctgcta gactttctaa
     1981 gtctcgtaga cttgagaacc ttatcgctca gcttccagga gagaagaaga acggactttt
     2041 cggaaacctt atcgctcttt ctcttggact taccccaaac ttcaagtcta acttcgatct
     2101 tgctgaggat gctaagttgc agctttctaa ggatacctac gatgatgatc ttgataacct
     2161 tcttgctcag atcggagatc agtacgctga tcttttcctt gctgctaaga acctttctga
     2221 tgctatcctt ctttctgaca tccttagagt taacaccgag atcaccaagg ctccactttc
     2281 tgcttctatg atcaagagat acgatgagca ccaccaggat cttacccttt tgaaggctct
     2341 tgttagacag cagcttccag agaagtacaa ggaaatcttc ttcgatcagt ctaagaacgg
     2401 atacgctgga tacatcgatg gaggagcttc tcaggaggag ttctacaagt tcatcaagcc
     2461 aatccttgag aagatggatg gaaccgagga gcttcttgtt aagttgaaca gagaggatct
     2521 tcttagaaag cagagaacct tcgataacgg atctatccca caccagatcc accttggaga
     2581 gcttcacgct atccttcgta gacaggagga tttctaccca ttcttgaagg ataacagaga
     2641 gaagatcgag aagatcctta ccttcagaat cccatactac gttggaccac ttgctagagg
     2701 aaactctcgt ttcgcttgga tgaccagaaa gtctgaggaa accatcaccc cttggaactt
     2761 cgaggaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt
     2821 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc
     2881 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa
     2941 atttgttgat gtgcaggttg ttgataaggg agcttctgct cagtctttca tcgagagaat
     3001 gaccaacttc gataagaacc ttccaaacga gaaggttctt ccaaagcact ctcttcttta
     3061 cgagtacttc accgtttaca acgagcttac caaggttaag tacgttaccg agggaatgag
     3121 aaagccagct ttcctttctg gagagcagaa gaaggctatc gttgatcttc ttttcaagac
     3181 caacagaaag gttaccgtta agcagttgaa ggaggattac ttcaagaaga tcgagtgctt
     3241 cgattctgtt gaaatctctg gagttgagga tagattcaac gcttctcttg gaacctacca
     3301 cgatcttttg aagatcatca aggataagga tttccttgat aacgaggaga acgaggacat
     3361 ccttgaggac atcgttctta cccttaccct tttcgaggat agagagatga tcgaggagag
     3421 actcaagacc tacgctcacc ttttcgatga taaggttatg aagcagttga agagaagaag
     3481 atacaccgga tggggtagac tttctcgtaa gttgatcaac ggaatcagag ataagcagtc
     3541 tggaaagacc atccttgatt tcttgaagtc tgatggattc gctaacagaa acttcatgca
     3601 gcttatccac gatgattctc ttaccttcaa ggaggacatc cagaaggctc aggtttctgg
     3661 acagggagat tctcttcacg agcacatcgc taaccttgct ggatctccag ctatcaagaa
     3721 gggaatcctt cagaccgtta aggttgttga tgagcttgtt aaggttatgg gtagacacaa
     3781 gccagagaac atcgttatcg agatggctag agagaaccag accacccaga agggacagaa
     3841 gaactctcgt gagagaatga agagaatcga ggagggaatc aaggagcttg gatctcaaat
     3901 cttgaaggag cacccagttg agaacaccca gcttcagaac gagaagttgt acctttacta
     3961 ccttcagaac ggaagagata tgtacgttga tcaggagctt gacatcaaca gactttctga
     4021 ttacgatgtt gatcacatcg ttccacagtc tttcttgaag gatgattcta tcgataacaa
     4081 ggttcttacc cgttctgata agaacagagg aaagtctgat aacgttccat ctgaggaggt
     4141 tgttaagaag atgaagaact actggagaca gcttcttaac gctaagttga tcacccagag
     4201 aaagttcgat aaccttacca aggctgagag aggaggactt tctgagcttg ataaggctgg
     4261 attcatcaag agacagcttg ttgaaaccag acagatcacc aagcacgttg ctcagatcct
     4321 tgattctcgt atgaacacca agtacgatga gaacgataag ttgatcagag aggttaaggt
     4381 tatcaccttg aagtctaagt tggtttctga tttcagaaag gatttccagt tctacaaggt
     4441 tagagagatc aacaactacc accacgctca cgatgcttac cttaacgctg ttgttggaac
     4501 cgctcttatc aagaagtacc caaagttgga gtctgagttc gtttacggag attacaaggt
     4561 ttacgatgtt agaaagatga tcgctaagtc tgagcaggag atcggaaagg ctaccgctaa
     4621 gtacttcttc tactctaaca tcatgaactt cttcaagacc gagatcaccc ttgctaacgg
     4681 agagatcaga aagaggccac ttatcgaaac caacggagaa accggagaga tcgtttggga
     4741 taagggaaga gatttcgcta ccgttagaaa ggttctttct atgccacagg ttaacatcgt
     4801 taagaaaacc gaggttcaga ccggaggatt ctctaaggag tctatccttc caaagagaaa
     4861 ctctgataag ttgatcgcta gaaagaagga ttgggaccca aagaagtacg gaggattcga
     4921 ttctccaacc gttgcttact ctgttcttgt tgttgctaag gttgagaagg gaaagtctaa
     4981 gaagttgaag tctgttaagg agcttcttgg aatcaccatc atggagcgtt cttctttcga
     5041 gaagaaccca atcgatttcc ttgaggctaa gggatacaag gaggttaaga aggatcttat
     5101 catcaagttg ccaaagtact ctcttttcga gcttgagaac ggaagaaaga gaatgcttgc
     5161 ttctgctgga gagcttcaga agggaaacga gcttgctctt ccatctaagt acgttaactt
     5221 cctttacctt gcttctcact acgagaagtt gaagggatct ccagaggata acgagcagaa
     5281 gcagcttttc gttgagcagc acaagcacta ccttgatgag atcatcgagc aaatctctga
     5341 gttctctaag agagttatcc ttgctgatgc taaccttgat aaggttcttt ctgcttacaa
     5401 caagcacaga gataagccaa tcagagagca ggctgagaac atcatccacc ttttcaccct
     5461 taccaacctt ggtgctccag ctgctttcaa gtacttcgat accaccatcg atagaaaaag
     5521 atacacctct accaaggagg ttcttgatgc tacccttatc caccagtcta tcaccggact
     5581 ttacgagacc agaatcgatc tttctcagct tggaggagat aagagaccag ctgctaccaa
     5641 gaaggctgga caggctaaga agaagaagtg aaaagctttc gttcgtatca tcggtttcga
     5701 caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt
     5761 ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     5821 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     5881 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     5941 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     6001 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag
     6061 ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca
     6121 aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat
     6181 gtaattttcc agaatccttg tcagattcta atcattgctt tataattata gttatactca
     6241 tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt
     6301 gacaacatgc atcaatcggt accacctagt agaagagctc agtgagaccg aattcgctta
     6361 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     6421 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc taagcttcgg atgccgggag
     6481 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     6541 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6601 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     6661 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     6721 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     6781 ggccagtgaa ttcgagctcg gtacacaggg atcttcaaga gtcgacctgc aggcatgcag
     6841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcac
     7141 tagtaggagg taatcaatgc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7201 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7261 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     7321 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     7381 cagtacaatc tgctctgatg ccgcatgatc atctggcgga cctcttcgga ggtccgcttt
     7441 tttttctcga gggtctcaac tagctcttca tgggtattct agagataatt tatttgaaaa
     7501 ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca
     7561 cgcacattta ggatattggc cgagattact gaatattgag taagatcacg gaatttctga
     7621 caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg
     7681 caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagagct
     7741 ctcacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact
     7801 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt
     7861 aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt
     7921 agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct
     7981 cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc
     8041 cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca
     8101 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
     8161 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
     8221 cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg
     8281 ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
     8341 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
     8401 gcatgaactc cttgatgatg gccatgttat ccattgttcg taccgttttg ctatttgtgt
     8461 atgttttctt gttttggttt gagtgaagag tgtagtgaag ggttggtttg tatttatagg
     8521 tgagaaagga gatttgcatg gcaatcacgt gtaagaatgc atgcatgcat gagtgtgtgt
     8581 ggagagagag agatgctctt acgttgttgt cgggattatg tgttggaaaa gagtgacaag
     8641 tgacgaacaa aacaatacca ctcatcatag ctccgcttat gcatgctatg tgtgatttgt
     8701 agctcttgtt ctgacatgac actatgaaca attttttaaa aagaaggtta aacttttatt
     8761 taaataaaaa ccctatgtta atttggtttc aatcgagtaa gatgagaagt caatcttttg
     8821 atggtagcta gtgatgttgg aaatatagtt gtgccagctt atctagacca agcatgcaca
     8881 gctggcgttc tcagccagat ccccttgaca ggatatattg gcgggtaaac taagtcgctg
     8941 tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9481 gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc cggcaaacaa
     9541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9721 aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg gtctagtgat
     9781 tatttgccga ctaccttggt gatctcgcct ttcacgtagt gaacaaattc ttccaactga
     9841 tctgcgcgcg aggccaagcg atcttcttgt ccaagataag cctgcctagc ttcaagtatg
     9901 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     9961 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
    10021 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
    10081 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
    10141 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
    10201 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
    10261 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
    10321 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
    10381 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
    10441 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
    10501 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
    10561 tccctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
    10621 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
    10681 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
    10741 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
    10801 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcgatcccc
    10861 atccaacagc ccgccgtcga gcgggct
//
