LOCUS       sequence-201149-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of luc, ekivR using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS35.41
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          2799..2914
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(2932..3549,3550..3555,3556..3591,3592..3594,3595..3630,
                     3631..3633,3634..3669,3670..3678)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(4026..4044,4045..4067,4068..4086,4087..4109,4110..4128,
                     4129..4151,4152..4170,4171..4193,4194..4212,4213..4235,
                     4236..4254,4255..4277,4278..4296)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          4366..4481
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             6248..6304
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(10359..10378)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10360..10378)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10388..10405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10388..10404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10397..10419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10546..11001
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10633..10652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10843..10864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcggagaa tatggagctt catcgaatca ccggcagtaa
     2281 gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc gaaatagcat gccattaacc
     2341 taggtacaga agtccaattg cttccgatct ggtaaaagat tcacgagata gtaccttctc
     2401 cgaagtaggt agagcgagta cccggcgcgt aagctcccta attggcccat ccggcatctg
     2461 tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg
     2521 ctcaggagct ggccagcggc gcagaccggg aacacaagct ggcagtcgac ccatccggtg
     2581 ctctgcactc gacctgctga ggtccctcag tccctggtag gcagctttgc cccgtctgtc
     2641 cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca gtccaacatt tgttgccata
     2701 ttttcctgct ctccccacca gctgctcttt tcttttctct ttcttttccc atcttcagta
     2761 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
     2821 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
     2881 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccgaattcac catgtctaga
     2941 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
     3001 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3061 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3121 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3181 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3241 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3301 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3361 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3421 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     3481 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     3541 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     3601 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     3661 gacatgctcc ccgggtaact aagtaaggat ccactagtac agcagaagaa tctctctccg
     3721 ctgttgcttc agtgtctgcc atgcattaac ttcatcctac tgtcctaccc gcagtaccca
     3781 ttcacatttg cggcagatac caggttgttt tctattccct cggttcttca gttcttcaga
     3841 tattacatta ctcgagaaat tggcggcgat gcaggagttt ggttggtttt aattgtttca
     3901 gtctccttga cctgatttgt atgacatgca atgtttcgac ggaagactat ctcggtgaat
     3961 atacgtcatg aatcatgcgg ccgcgcgtat cacgaggccc tttcgtcttc actcgagttt
     4021 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     4081 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4141 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4201 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4261 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccccatc
     4321 ttcagtatat tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta
     4381 catccatact ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc
     4441 acttcatcgc agcttgacta acagctaccc cgcttgagca gacatcaccg tttaaacacc
     4501 atggaggacg ccaagaacat caagaagggc cccgccccct tctaccccct cgaggacggc
     4561 accgccggcg agcagctcca caaggccatg aagcgctacg ccctcgtccc cggcaccatc
     4621 gccttcgtag gtttcctcca gctctcgcct ccagcacccg aggcacacct cgggcatctt
     4681 cacaacaaca gacactgaca tctcattctc acagaccgac gcccacatcg aggtcaacat
     4741 cacctacgcc gagtacttcg agatgtccgt ccgcctcgcc gaggccatga agcgctacgg
     4801 cctcaacacc aaccaccgca tcgtcgtctg ctccgagaac tccctccagt tcttcatgcc
     4861 cgtcctcggc gccctcttca tcggcgtcgc cgtcgccccc gccaacgaca tctacaacga
     4921 gcgcgagctc ctcaactcca tgaacatctc ccagcccacc gtcgtcttcg tctccaagaa
     4981 gggcctccag aagatcctca acgtccagaa gaagctcccc atcatccaga agatcatcat
     5041 catggactcc aagaccgact accagggctt ccagtccatg tacaccttcg tcacctccca
     5101 cctccccccc ggcttcagcg agtacgactt cgtccccgag tccttcgacc gcgacaagac
     5161 catcgccctc atcatgaact cctccggctc caccggcctc cccaagggcg tcgccctccc
     5221 ccaccgcacc gcctgcgtcc gcttctccca cgcccgcgac cccatcttcg gcaaccagat
     5281 catccccgac accgccatcc tctccgtcgt ccccttccac cacggcttcg gcatgttcac
     5341 caccctcggc tacctcatct gcggcttccg cgtcgtcctc atgtaccgct tcgaggagga
     5401 gctcttcctc cgctccctcc aggactacaa gatccagtcc gccctcctcg tccccaccct
     5461 cttctccttc ttcgccaagt ccaccctcat cgacaagtac gacctctcca acctccacga
     5521 gatcgcctcc ggcggcgccc ccctctccaa ggaggtcggc gaggccgtcg ccaagcgctt
     5581 ccacctcccc ggcatccgcc agggctacgg cctcaccgag accacctccg ccatcctcat
     5641 cacccccgag ggcgacgaca agcccggcgc cgtcggcaag gtcgtcccct tcttcgaggc
     5701 caaggtcgtc gacctcgaca ccggcaagac cctcggcgtc aaccagcgcg gcgagctctg
     5761 cgtccgcggc cccatgatca tgtccggcta cgtcaacaac cccgaggcca ccaacgccct
     5821 catcgacaag gacggctggc tccactccgg cgacatcgcc tactgggacg aggacgagca
     5881 cttcttcatc gtcgaccgcc tcaagtccct catcaagtac aagggctacc aggtcgcccc
     5941 cgccgagctc gagtccatcc tcctccagca ccccaacatc ttcgacgccg gcgtcgccgg
     6001 cctccccgac gacgacgccg gcgagctccc cgccgccgtc gtcgtcctcg agcacggcaa
     6061 gaccatgacc gagaaggaga tcgtcgacta cgtcgcctcc caggtcacca ccgccaagaa
     6121 gctccgcggc ggcgtcgtct tcgtcgacga ggtccccaag ggcctcaccg gcaagctcga
     6181 cgcccgcaag atccgcgaga tcctcatcaa ggccaagaag ggcggcaagt ccaagctcgg
     6241 ctccggcgcc accaacttct ccctcctgaa gcaggccggt gacgtcgagg agaaccccgg
     6301 ccccatggaa aagcctgtgg cctcacaagt ccaccccagc tggctaacag ctcttctacc
     6361 tgaatccaga atacccccca agctgtttgc ctggtcctca gccaacatcc gcccagcaag
     6421 ctcctccaag ctcggaggct ctgccaccaa catccaccat gcagagtgtt cacatccaac
     6481 cccaaactcc acagcaagct ctgagcagcg catcttcatc gttggtcccg gcaacatagg
     6541 taggctctac gcaagccaca tggtccgccg cccaaatccc ctccccatca ctctcgtcgt
     6601 ccccagaaag gaactcctat cgcaatgggc aggctgtgaa ggcgtgggcc ttatagaccc
     6661 gggtcgcggc aaggtcctca aaagcaagcg cttcaccgtc gagtggtgga cggagactag
     6721 accacagtat ggtcctgtga gagaggtggc agacggaaag aagctgcgca acgtgttcat
     6781 atccacaaag gcatccgctg ggctggccga ggcagatcgt cttcgtcggt atttgggcaa
     6841 gtgtagttct gtcgtctttg cgcagaacgg agtatgcaag ctgtggcctc ctcatgggcc
     6901 gttatacgtc gccggtcgat accctcctgg tgatgctcct accttctcat cctgcgtcgt
     6961 gaatcatggc gttgcatcag cggggccttt tctgagcatt cacgcagctc cggcagatgc
     7021 atccatcgga cctgtcctct ggccttccca tccgatgccg cacaagaaac ccctgaacga
     7081 cttcttcaca agatacattg ctactacacc cttcctaaac acaaagcaag tttcctctgg
     7141 tgagctctgg cttattcagc tggagaaact tgttaccaat gccgccatca accccctcac
     7201 agctttgcta cgctgcaaaa cgggacagct gttcacaacc catgacccca cagaccctct
     7261 tacgcgcgtg ctggacaggt tgctatggca ggccagcgcc gtgattcagg gtcttatcaa
     7321 ccacgacgcg agtgtcgaca tcgtcgcttc ctacgccgag cacctccacc agaccgaacc
     7381 aagcagcgac tcaagccttg tcgcagtacg gaccaagtta acagagcggt tctcccagcc
     7441 taccttgaga gcgaagctat tcgcctttgg tcgcaagata tacgagcatc gcagctcgat
     7501 gcttcaggat gttgaggccg gcaggaaaac ggagataaga gatttcaacg ggtgggttgt
     7561 ggatatggcg agttttctgg ggacggggct gaatgtgagc gtgcatcaga gtttgattgt
     7621 gctgattgaa cgcggtgagg ttttgaacaa ggatgagttg gctaggagga cattataagt
     7681 ttaaacagta gatgccgacc gggatccact taacgttact gaaatcatca aacagcttga
     7741 cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga caaatggtgt
     7801 tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct tctagtgatt
     7861 taatagctcc atgtcaacaa gaataaaacg cgtttcgggt ttacctcttc cagatacagc
     7921 tcatctgcaa tgcattaatg cattggacct cgcaacccta gtacgccctt caggctccgg
     7981 cgaagcagaa gaatagctta gcagagtcta ttttcatttt cgggagacga gatcaagcag
     8041 atcaacggtc gtcaagagac ctacgagact gaggaatccg ctcttggctc cacgcgggcg
     8101 cgcctcggtc gctcactgtt cctttacgga tatgtgtact ccggcaagcc gcggggtcgc
     8161 ggtcgtttgt acggcagctt ctacttgctt gcacagggag ctctctgggg cttgacgtct
     8221 tttggagttg cgagggagtt gatttcctac ttctaagttt ggactgaatc cgtggtgtga
     8281 ttgaggtgat tggcgatgtt tggctatacc agctatatgt aataatctct actgtatact
     8341 actattcaac gcattttact atgcgtgctg ctagggtcgg caatgacaat ggcaatctga
     8401 ctgacgtggt ctatttctcc atgtgcagca gggaatacga gctccaatgg acctcgggag
     8461 tggcacagtc aatggcaagg aaactccgcc tttgcaggtg tggctgaacc ccacgggtcg
     8521 gaggcggagc aatccacccc cgatgtggct ggtgcgtgga ggggctcgcg atgattttac
     8581 tgagcttgct tttcttgtcg acattgaaca ttgtccttgg tcttccttca gatttaaggg
     8641 tcagtcactg ctacatttct cagtagtatc cgcgcacgtc tctggattta cgaatcaggg
     8701 tccaccagtc gaaacttcga actactctca ttatacaatc ctctttccat tcccgcatta
     8761 acccctccat caacaccatg tcctccaagt cgcaattgac ctacactgcc cgtgccagca
     8821 agcatcccaa tgctctggcg aagaggctgt tcgagattgc cgaggccaag aagaccaatg
     8881 tgactgtctc ggctgacgtt accaccacta aggagctact agatcttgct gaccgtaggc
     8941 cgacccgcta ctctgcctga ttatgctgca tgcaaactta ttaacggtga taccggactg
     9001 caggtctcgg tccctacatt gccgtgatca aaacccacat cgatatcctc tctgatttca
     9061 gcaacgagac cattgaggga cttaaggctc tcgcgcagaa gcacaacttt ctcatcttcg
     9121 aggaccgcaa gttcattgac atcggcaaca cggtccagaa gcaataccac ggcggtaccc
     9181 tccgtatctc ggaatgggcc cacatcatca actgcagcat tctccctggt gagggtatcg
     9241 tcgaggctct cgctcagacg gcgtctgcac cggacttcgc ctacggcccc gaacgcggtc
     9301 tgttgatctt ggcagagatg acctctaagg gctccttggc taccggccag tacactactt
     9361 cctcggtcga ttatgcccgg aaatacaaga acttcgttat gggattcgtg tcgacgcgcg
     9421 cgttgggtga ggtgcagtcg gaagtcagct ctccttcgga tgaggaggac tttgtggtct
     9481 tcacgactgg tgtgaacatt tcttccaagg gagataagct tggtcagcag taccagacgc
     9541 ccggatcggc tatcggccgg ggtgctgact tcattatcgc gggtcgcggt atctacgccg
     9601 cgccggatcg atccggtgca ggctgcgcaa cagtatcaga aggaggggtg ggaagcctac
     9661 ctggcccgtg tcggcggaaa ctaatactat aaaaggagga tcgaagttct gatggttatg
     9721 aatgatatag aaatgcaact tgccgcaacg gatacggaag cggaaacgga ccaatgtcga
     9781 gcacgggtag tcagactgcg gcatcggatg tccaaacggt attgatcctg caggctacta
     9841 tggtgtggca caaggatcaa tgcggtacga cgatttgatg cagataagca ggctgcgaag
     9901 tagtaactct tgcgtagaga aaatggcgac gggtgggctg ataagggcgg tgataagctt
     9961 aattgtcatc gcagataagc actgctgtct tgcatccaag tcagcgtcag cagaaatacg
    10021 ggacttccga aagtatatgg caaaattaaa gaacttgact ctccagcaat gttttgccct
    10081 gaccgtcgct aaaacgttac tacccctata cccgtctgtt tgtcccagcc cgaggcatta
    10141 ggtctgactg acagcacggc gccatgcggg cttgggacgc catgtccgtc gcgtgataag
    10201 ggttgatcca tgcagctact atccttccat cgttccattc ccatccttgt cctatctcca
    10261 tccttgaaac tttactagtt tagttggatg ctcgagcttg ctctcggcta ctccgtcggc
    10321 gcgccttgcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattacg
    10381 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    10441 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    10501 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
    10561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    10621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    10681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    10981 aaatattaac gcttacaatt tag
//
