LOCUS       sequence-201150-       20465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of luc, ekivR, esyn1, mutated P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 20465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS36.6
FEATURES             Location/Qualifiers
     source          1..20465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          2799..2914
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(2932..3549,3550..3555,3556..3591,3592..3594,3595..3630,
                     3631..3633,3634..3669,3670..3678)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(4026..4044,4045..4067,4068..4086,4087..4109,4110..4128,
                     4129..4151,4152..4170,4171..4193,4194..4212,4213..4235,
                     4236..4254,4255..4277,4278..4296)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          4366..4481
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     primer_bind     complement(19821..19840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(19822..19840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(19850..19867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(19850..19866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19859..19881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      20008..20463
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(20095..20114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     20305..20326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcggagaa tatggagctt catcgaatca ccggcagtaa
     2281 gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc gaaatagcat gccattaacc
     2341 taggtacaga agtccaattg cttccgatct ggtaaaagat tcacgagata gtaccttctc
     2401 cgaagtaggt agagcgagta cccggcgcgt aagctcccta attggcccat ccggcatctg
     2461 tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg
     2521 ctcaggagct ggccagcggc gcagaccggg aacacaagct ggcagtcgac ccatccggtg
     2581 ctctgcactc gacctgctga ggtccctcag tccctggtag gcagctttgc cccgtctgtc
     2641 cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca gtccaacatt tgttgccata
     2701 ttttcctgct ctccccacca gctgctcttt tcttttctct ttcttttccc atcttcagta
     2761 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
     2821 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
     2881 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccgaattcac catgtctaga
     2941 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
     3001 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3061 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3121 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3181 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3241 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3301 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3361 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3421 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     3481 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     3541 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     3601 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     3661 gacatgctcc ccgggtaact aagtaaggat ccactagtac agcagaagaa tctctctccg
     3721 ctgttgcttc agtgtctgcc atgcattaac ttcatcctac tgtcctaccc gcagtaccca
     3781 ttcacatttg cggcagatac caggttgttt tctattccct cggttcttca gttcttcaga
     3841 tattacatta ctcgagaaat tggcggcgat gcaggagttt ggttggtttt aattgtttca
     3901 gtctccttga cctgatttgt atgacatgca atgtttcgac ggaagactat ctcggtgaat
     3961 atacgtcatg aatcatgcgg ccgcgcgtat cacgaggccc tttcgtcttc actcgagttt
     4021 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     4081 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4141 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4201 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4261 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccccatc
     4321 ttcagtatat tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta
     4381 catccatact ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc
     4441 acttcatcgc agcttgacta acagctaccc cgcttgagca gacatcaccg tttaaacacc
     4501 atggaggacg ccaagaacat caagaagggc cccgccccct tctaccccct cgaggacggc
     4561 accgccggcg agcagctcca caaggccatg aagcgctacg ccctcgtccc cggcaccatc
     4621 gccttcgtag gtttcctcca gctctcgcct ccagcacccg aggcacacct cgggcatctt
     4681 cacaacaaca gacactgaca tctcattctc acagaccgac gcccacatcg aggtcaacat
     4741 cacctacgcc gagtacttcg agatgtccgt ccgcctcgcc gaggccatga agcgctacgg
     4801 cctcaacacc aaccaccgca tcgtcgtctg ctccgagaac tccctccagt tcttcatgcc
     4861 cgtcctcggc gccctcttca tcggcgtcgc cgtcgccccc gccaacgaca tctacaacga
     4921 gcgcgagctc ctcaactcca tgaacatctc ccagcccacc gtcgtcttcg tctccaagaa
     4981 gggcctccag aagatcctca acgtccagaa gaagctcccc atcatccaga agatcatcat
     5041 catggactcc aagaccgact accagggctt ccagtccatg tacaccttcg tcacctccca
     5101 cctccccccc ggcttcagcg agtacgactt cgtccccgag tccttcgacc gcgacaagac
     5161 catcgccctc atcatgaact cctccggctc caccggcctc cccaagggcg tcgccctccc
     5221 ccaccgcacc gcctgcgtcc gcttctccca cgcccgcgac cccatcttcg gcaaccagat
     5281 catccccgac accgccatcc tctccgtcgt ccccttccac cacggcttcg gcatgttcac
     5341 caccctcggc tacctcatct gcggcttccg cgtcgtcctc atgtaccgct tcgaggagga
     5401 gctcttcctc cgctccctcc aggactacaa gatccagtcc gccctcctcg tccccaccct
     5461 cttctccttc ttcgccaagt ccaccctcat cgacaagtac gacctctcca acctccacga
     5521 gatcgcctcc ggcggcgccc ccctctccaa ggaggtcggc gaggccgtcg ccaagcgctt
     5581 ccacctcccc ggcatccgcc agggctacgg cctcaccgag accacctccg ccatcctcat
     5641 cacccccgag ggcgacgaca agcccggcgc cgtcggcaag gtcgtcccct tcttcgaggc
     5701 caaggtcgtc gacctcgaca ccggcaagac cctcggcgtc aaccagcgcg gcgagctctg
     5761 cgtccgcggc cccatgatca tgtccggcta cgtcaacaac cccgaggcca ccaacgccct
     5821 catcgacaag gacggctggc tccactccgg cgacatcgcc tactgggacg aggacgagca
     5881 cttcttcatc gtcgaccgcc tcaagtccct catcaagtac aagggctacc aggtcgcccc
     5941 cgccgagctc gagtccatcc tcctccagca ccccaacatc ttcgacgccg gcgtcgccgg
     6001 cctccccgac gacgacgccg gcgagctccc cgccgccgtc gtcgtcctcg agcacggcaa
     6061 gaccatgacc gagaaggaga tcgtcgacta cgtcgcctcc caggtcacca ccgccaagaa
     6121 gctccgcggc ggcgtcgtct tcgtcgacga ggtccccaag ggcctcaccg gcaagctcga
     6181 cgcccgcaag atccgcgaga tcctcatcaa ggccaagaag ggcggcaagt ccaagctcgg
     6241 ctccggcgcc accaacttct ccctcctgaa gcaggccggt gacgtcgagg agaaccccgg
     6301 cgccatggaa aagcctgtgg cctcacaagt ccaccccagc tggctaacag ctcttctacc
     6361 tgaatccaga atacccccca agctgtttgc ctggtcctca gccaacatcc gcccagcaag
     6421 ctcctccaag ctcggaggct ctgccaccaa catccaccat gcagagtgtt cacatccaac
     6481 cccaaactcc acagcaagct ctgagcagcg catcttcatc gttggtcccg gcaacatagg
     6541 taggctctac gcaagccaca tggtccgccg cccaaatccc ctccccatca ctctcgtcgt
     6601 ccccagaaag gaactcctat cgcaatgggc aggctgtgaa ggcgtgggcc ttatagaccc
     6661 gggtcgcggc aaggtcctca aaagcaagcg cttcaccgtc gagtggtgga cggagactag
     6721 accacagtat ggtcctgtga gagaggtggc agacggaaag aagctgcgca acgtgttcat
     6781 atccacaaag gcatccgctg ggctggccga ggcagatcgt cttcgtcggt atttgggcaa
     6841 gtgtagttct gtcgtctttg cgcagaacgg agtatgcaag ctgtggcctc ctcatgggcc
     6901 gttatacgtc gccggtcgat accctcctgg tgatgctcct accttctcat cctgcgtcgt
     6961 gaatcatggc gttgcatcag cggggccttt tctgagcatt cacgcagctc cggcagatgc
     7021 atccatcgga cctgtcctct ggccttccca tccgatgccg cacaagaaac ccctgaacga
     7081 cttcttcaca agatacattg ctactacacc cttcctaaac acaaagcaag tttcctctgg
     7141 tgagctctgg cttattcagc tggagaaact tgttaccaat gccgccatca accccctcac
     7201 agctttgcta cgctgcaaaa cgggacagct gttcacaacc catgacccca cagaccctct
     7261 tacgcgcgtg ctggacaggt tgctatggca ggccagcgcc gtgattcagg gtcttatcaa
     7321 ccacgacgcg agtgtcgaca tcgtcgcttc ctacgccgag cacctccacc agaccgaacc
     7381 aagcagcgac tcaagccttg tcgcagtacg gaccaagtta acagagcggt tctcccagcc
     7441 taccttgaga gcgaagctat tcgcctttgg tcgcaagata tacgagcatc gcagctcgat
     7501 gcttcaggat gttgaggccg gcaggaaaac ggagataaga gatttcaacg ggtgggttgt
     7561 ggatatggcg agttttctgg ggacggggct gaatgtgagc gtgcatcaga gtttgattgt
     7621 gctgattgaa cgcggtgagg ttttgaacaa ggatgagttg gctaggagga cattaggctc
     7681 cggcgccacc aacttctccc tcctgaagca ggccggtgac gtcgaggaga accccggcgc
     7741 catgtcactc cacaccccaa gtgacggtca acaagaccca gcgttagcct ccaaaaccct
     7801 ctgtgagcaa atctcgcgag ccctcggcct cggccaggac aagattgaga atatcttccc
     7861 agggacaccc ttccagcggg acgtcataga ctgcgccgca gacgacaagc agcgcgccgt
     7921 gggccacgct gtctttgaaa ttcccaaaga tatagacgcg gcccgcctgg cggctgcgtg
     7981 gaaggagacc gtgcttcaca cccctgctct gcggacctgc acgtttacgt ccaaatctgg
     8041 ggatgtgctt caggtggtcc tgagagatag ctttgtcttt tcgtggatgt ctgggccgtc
     8101 tgtggatctt aaggaggcgg ttgtgcaaga cgaggctgct gctgctttgg ctggcccgcg
     8161 ctgtaaccgc ttcgtccttc ttgaggaccc tgatacaaag gaacgtcagc tcatctggac
     8221 gttcagccat gctctcgttg atagtacctt ccaggaacgt atccttcgac gagtcctcaa
     8281 agcttacaag gatgccaatg acgaacatcc tcgccagttc gagacaccag attcttccca
     8341 ggccacgccc gaggaggatt tacagcccaa tccatccaag atgctcaaga tccctcaagc
     8401 cgcagatatg gaccgtgctg ttgagttttg gaaggatcat ctaagtggcc tgaatgcttc
     8461 tgccttcccg catttgtcct ctcatctgtc tatgccacat ccagatgcca aggcagagca
     8521 tagaatctcc tactcgtctt cagcacagca aaaaatgtcc agtgctacta tttgccgaac
     8581 tgccctggca attctgttat ctcgttatac gcactctcct gaagcactct ttggcattgt
     8641 aactgagcaa actcctctac tggaggagca actcatgctt gatggcccaa cacgtacagt
     8701 cgtaccaatc cgcgtatcct gtgcttctga acagtcagtg tcagatatca tgagcacgat
     8761 tgactcctac gatcagacca tgcggcagtt tgcccacgct ggtcttcgca acatcgccag
     8821 tgctggagac gatgaatctg ccgcctgcgg gttccagacc gttctcttgg tctcagatgg
     8881 agacgcccag ccagcatcta cttgggaaat tctcaagaaa actgaagagc ctgagggttt
     8941 catcccctgt acgaaccgtg ctctgcttct aagttgccag atgaccagct ctggagcaca
     9001 cctaaccgcc agatacgacc agagtatcat cgacgctgag caaatggccc gactcctgcg
     9061 acaactaggc catctgatcc aaaatcttca aacctccaca gatctacccg tggaaaaggt
     9121 ggacatgatg acgcaagaag actggttaga gattgagagg tggaactcag actccatcga
     9181 cgcacaagac acgcttatcc acagcgagat gttgaagtgg acttctcagt cgcctaacaa
     9241 agctgccgtc gccgcgtggg atggagagtg gacgtatgct gagctggaca atgtatcttc
     9301 gcgcctggct cagcatatca actctattga cctgggcaag gagcacgcga tagtacccat
     9361 ctactttgag aaatcgaaat gggtcgttgc ttcgatgctg gctgtgctca aggctggcca
     9421 tgcgtttact ctcattgacc ccagtgatcc accagctcga acagcacagg tcgtgcagca
     9481 aacctctgcg acagttgctc ttacttccaa gctccatcgc gagactgttc aatcaactgt
     9541 cgggcgctgc attgttgtcg acgaagagtt tgtcaaatcc ctcccccagt ccagcgaact
     9601 ctcagcgtct gtaaaggcac atgacctggc atatgtcatc ttcacctcgg gcagcacagg
     9661 cattccaaag ggaatcatga ttgagcaccg ctcattttca tcctgcgcca tcaaattcgg
     9721 tcccgcgctt ggaataacct ccgacacacg cgctcttcag tttggatctc atgcctttgg
     9781 cgcgtgtatc ctcgagatca tgacgacgct catccacgga ggctgtgtct gtatcccctc
     9841 tgacgatgac cgcatgaaca acgtccttga gttcatcaac cggactaacg tcaactgggt
     9901 catggcaacg ccttcgtaca tggggacatt ccagccagag gttgttcctg gtttgaagac
     9961 cttggtattg gtaggtgagc agatgtctgc ttctgtgaat gaggtctggg ctcctcgtgt
    10021 tcaactcctg aacggttacg gacagagcga gagttcttct atctgctgcg tagccaagat
    10081 cagccctggt tcatctgagc caaacaatat tggccacgca gtaggcgcac attcttggat
    10141 cgttgaccct gaggatccca atcgcttggc tcccatcggt gccgttggcg agctggtcat
    10201 cgagagtgca ggtatcgcac gcgattacat cgttgcgcca actcaggata agtctccttt
    10261 cattaagact gctcctacgt ggtatccggc caagcagctc ccggacggct tcaagatcta
    10321 cagaacaggc gatcttgcat gctacgcatc tgatggcagt atagtgtgcc ttggccgtat
    10381 ggactcccag gtcaagatca gaggacagcg tgttgagctg ggtgcagtgg agactcatct
    10441 tcgacagcag atgcctgatg atatgaccat cgttgttgaa gctgtcaagt tctctgattc
    10501 ctccagtact acagttttga cagccttctt gattggtgca ggggagaaga acagtcatat
    10561 cttggaccag cgtgctacaa gggagatcaa tgccaagatg gagcaagttc ttcctcgcca
    10621 ttccatcccc gcgttctaca tctctatgaa caacctcccc caaacagcca ctggtaaggt
    10681 cgatcgacga aaacttcgta tcatgggtag caagatactg agccaaaaga cgcattccac
    10741 cccatctcag cagagtcagg ccgccatctc atctggaacc gacacagaga ccaagctgga
    10801 gagcatctgg atcacaagct tggacctcga gcccggctct gcaaacatgt cagctacatt
    10861 cttcgagatg ggcggaaact ccatcatcgc aatcaagatg gtcaacatgg cgcgctccaa
    10921 tggcatagag cttaaggtct cggacattta ccagaacccc acgctcgctg gtctcaaggc
    10981 tattgtcatt ggtacttcgc tgccatacag ccttattccc aaggttaccc gccaaggccc
    11041 tgttagcgag cagtcttatg cgcaaaacag aatgtggttc ctggatcagt tgtctgaggg
    11101 tgcttcatgg tatctgattc ctttcgctgt gcgcatgcga ggtccggtgg atgttgatgc
    11161 gttgacgcgt gctttgctcg ctcttgaaca gcgtcatgag acactacgaa ctacatttga
    11221 gaaccaagat ggtgttggag tccagatcat ccatgataga ctctccaaag agctacaagt
    11281 catcgatgcc ttggatggtg acgagggtgg tctcaagaca ctctacaaag tagagaccac
    11341 cacattcgac atcacatccg aagcaggctg gagctcaacc ctcatccgcc tcggcaaaga
    11401 cgatcacatt ctgtctatcg tcatgcacca catcatctcc gacggctggt ccatcgacgt
    11461 tctccgccgc gaactcatcc aactctacgc cgccgctctg cagggcaagg atccttcctc
    11521 cgcactaacc cccctaccca tccagtacag cgacttcgcc gtgtggcaga agcaggaggc
    11581 ccaagcagct gagcacgaga ggcagctcca gtactggaag aagcaactcg cagatagttc
    11641 acctgccaag atccctaccg acttcccccg tccagatctc ctgtccggtg acgcaggcgt
    11701 tgtgcccgtt gccatcgacg gcgagctgta tcagaaacta aggggcttct gcaacaagca
    11761 caacagcact gccttctcca tcctgctcgc tgctttccgc gcggcgcatt accgtctcac
    11821 agccgttgac gacgccgtga tcggcatccc cattgcaaac cgtaaccgct gggagctgga
    11881 gaacatgatt ggtttctttg tcaacacgca gtgtatgcgc atcgccgttg acgagacgga
    11941 tacatttgag agtctggtgc gccaggtcag atctaccact acagctgcgt ttgcgcacga
    12001 ggatgtcccc ttcgagcgtg tcgtttcagc gcttcagcct ggccatagag atctctcgcg
    12061 aacaccgctg gcacagataa tgtttgctgt tcactcgcag aaggaccttg gacgtttcga
    12121 gctggagggt atccagtctg agcctatcgc cagcaaggcc tacaccagat tcgatgtcga
    12181 gttccatctg ttccaacagg cagacggact gaagggcagt tgcaactttg ccacagatct
    12241 cttcaagccc gaaactatcc agaatgttgt cagcgtgttt ttccagattc tacgccatgg
    12301 ccttgaccag cctgagacgt gtatctcggt tcttccactg actgatggag tcgaggagct
    12361 tcgcaggttg gatctgctgg aaatcaagag gactaactac cctcgcgatt cgagcgtggt
    12421 agatgtcttc cgcgaacaag ctgccgccaa cccagaggtt atcgctgtta ccgactcatc
    12481 ttctcgtctg acttatgcag agctggacaa taagtctgag ctgctctcac gatggcttcg
    12541 acgacgtaac ttgacgccag agacgctggt cagtgttctt gctccccggt cttgcgagac
    12601 tatcgttgcc tatgttggta tcctcaaggc gaacctggcg tatcttcctc ttgacgtgag
    12661 gtccccggtg actcgtatga aggacatctt gtcgagcgtg tctggaaaca ccatagttct
    12721 tatgggctct ggggtagagg atcctggctt tgacttaccg caactagagc tcgtacgcat
    12781 caccgacact ttcgatgaga ccatcgagga cgtgcaagac tctccccaac cgtctgccac
    12841 aagcctcgcc tacgtcgtct tcacatccgg ttcaactggt aaacccaagg gcgtcatgat
    12901 cgagcaccgg gccattgtgc gtctcgtcaa gagtgacaac tttcctggct tcccctcccc
    12961 cgctcgcatg tcaaatgtct tcaaccctgc cttcgacgga gccatctggg agatcaactg
    13021 gatgctcctg aacggcggaa cagtcgtctg catcgactac ctgaccaccc tggacggcaa
    13081 agagctcgct gctgtgttcg ccaaagagcg cgtcaacgcc gccttcttcg cacctgcgat
    13141 gctcaagctt tacctcgttg atgcgcgcga ggctttgaag aatcttgact tccttattgt
    13201 tggaggtgag aggttcgata ccaaggaagc cgtggaggcc atgccgcttg tgagaggcaa
    13261 gattgccaat atctatggtc cgactgaggc tggaataatc agcacgtgct ataacatccc
    13321 caaggatgag gcttacacca atggtgttcc cattggtgga agtatctaca actctggtgc
    13381 ctacgtcatg gatcctaacc agcaacttgt cggccttggc gtcatgggag agcttgttgt
    13441 taccggagac ggtgttggtc gaggctacac taaccccgag ctcaacaaga accgcttcat
    13501 cgacatcacc atcgagggca agactttcaa ggcttaccgt actggtgacc ggatgcgtgc
    13561 acgagtaggc gacggtctcc ttgagttctt cggccgcatg gacaaccagt tcaagatccg
    13621 cggcaaccgt atcgaagcag gggaagttga gtctgccatg ctcagcctca agaatgtcct
    13681 taacgccgcc attgtcgtcc gcgggggcgg agaagatgaa gggccactcg agatggtcgg
    13741 attcatcgtc gcggacgaca agaatgatac cacggaggag gaagagacag gcaaccaagt
    13801 tgagggctgg caggaccatt tcgagagtgg tatgtactcg gatatcagca ccgccgtgga
    13861 ccaatctgcc attggaaacg actttaaggg ctggacttct atgtacgatg gtaaggatat
    13921 cgacaagggc gagatgcagg agtggttgga cgacgctatt cacaccctgc ataacggcca
    13981 gatccctcgc gatgtcctcg agatcggtac cggtagtggt atgatcctgt tcaacctcaa
    14041 cccgggcctc aacagctacg ttggtcttga tccatccaag tcagcagtcg agttcgtcaa
    14101 cagagccgtc gagtcctctc ccaagttcgc aggaaaagcc aaggtccacg tcggcatggc
    14161 cacagacgtc aacaaactcg gcgaagtaca ccccgatctc gtggtcttca actccgttgt
    14221 tcaatacttc cccacacccg agtatctcgc cgaggtcatc gatggcctca ttgccatccc
    14281 cagcgtcaag cgcatcttcc ttggcgatat cagatcatat gctaccaacg gacacttcct
    14341 cgccgcacgc gccatccaca cgctcggcac caataacaac gccaccaagg atagggtgcg
    14401 ccagaagatc caggagctgg aggatcgaga ggaagagttt ctcgttgagc ctgccttctt
    14461 caccactctg aaggagcgac gtccagatgt tgtcaagcat gttgagatca tccccaagaa
    14521 catgaaggcc accaacgaac tcagcgccta tcgctacacg gctgttgtgc atctgcggga
    14581 tgaaacggac gaacctgtgt atcatattga gaaggatagc tgggttgact ttgaggcgaa
    14641 gcagatggac aagacggctc ttcttgacca cctgcgcctc tccaaggatg ctatgagtgt
    14701 ggcggttagc aacatcacct acgcccacac tgcctttgaa cgtcgtatcg ttgagtctct
    14761 cgatgaggat agcaaggatg acaccaaggg tacactcgat ggtgcagcct ggctctcagc
    14821 agttcgctcc gaagccgaaa accgcgcctc actcaccgtc cccgacatcc tggagatcgc
    14881 caaagaggct ggtttccgag ttgaagtcag cgctgctcgc cagtggtccc aaagtggtgc
    14941 tttagacgca gtcttccacc actttccacc ctccagcact gaccgcactc taatccagtt
    15001 ccccacggac aacgagcttc gatcatcact caccctcgcc aaccgccctc tccagaagct
    15061 gcagcgccgt cgtgccgctc tgcaagtccg cgagaagctc cagacgctgg tcccgtctta
    15121 catggttcct ccgaatatcg tggtgctgga cacgatgcct ctcaatacta acggcaagat
    15181 cgacagaaag gagcttacgc gtagagcacg aacactgccg aagcagcaga ctgcggcgcc
    15241 tgtgccggac ttccctatct ctgatatcga gatcacgctg tgcgaggagg caactgaggt
    15301 ctttggaatg aaggttgaaa tcagcgatca cttcttccag ctcggcggtc actctctcct
    15361 cgctacgaaa ctcatttctc gcatccagca ccgtctccat gtgcgggtta ctgtgaagga
    15421 cgtattcgac agccctgtct ttgccgatct ggcagtcatc atccgtcaag gacttgctat
    15481 gcagaaccct gttgctgaag gacaggacaa gcaaggctgg tcctcgagag ttgcccctcg
    15541 tacagaagtc gagaagatgc tgtgcgagga gttcgcagct ggtcttggtg tcccggttgg
    15601 tatcactgac aacttcttcg atctcggtgg tcactcgctc atggctacta agctagctgt
    15661 gcgaattggc cgtcgccttg ataccgccat cacagtcaag gacatcttcg actaccctgt
    15721 gcttttccaa ttggcgaaga agttggagtc ttcgcattcc aagagctacg aggagtctgg
    15781 cgacgatatc cagatggccg attacactgc attccagctc ctcgatctgg aagaccccca
    15841 agactttgtt cagtcccaga ttcggcctca actggactcc tgctacggca ccatccagga
    15901 tgtctacccg tctacgcaga tgcaaaaggc cttcctcttc gatcccacga ccggcgagcc
    15961 ccgaggtctt gtgcctttct atatcgactt ccccagcaat gcagatgccg agaccctcac
    16021 caaggctatc ggagctctag ttgacaagct cgatatgttc agaactgtct tcctggaggc
    16081 cgcaggcgat ctgtaccaag ttgtcgttga gcacctcaac ttgcctattg agaccatcga
    16141 gactgagaag aacgtcaaca ctgcaaccgg tgactacctg gatgttcatg gaaaggaccc
    16201 tgtccgtcta ggccacccgt gcatccaatt cgccatcctg aagactgcct cctctgtacg
    16261 tgttctcctg cgaatgtccc acgctctgta tgatggtttg agttttgagt acatcgtccg
    16321 tggtctccac gttctctaca gcggtagaaa cctcccccca ccaacacagt ttgcgcgata
    16381 catgcagtat gctgcacaca gtcgtgaaga aggttatccc ttctggcgcg aggttctgca
    16441 gaacgcgccc atgacagttc tacacgacac caataacggt atgtctgagc aagagatgcc
    16501 agcctccaag gcggtacacc tgtcagaggt cgtcaacgtt ccagcgcaag ctattcgaaa
    16561 cagtaccaac acccaagcga ccgtcttcaa caccgcctgt gcccttgtcc tagccaagga
    16621 atccggctca caggatgtcg tcttcggccg tattgtctct ggtcgacaag gtctaccagt
    16681 cgtctggcag gacatcatcg gcccctgcac aaacgccgtg cccgtccacg cacgcgtcga
    16741 cgatggaaac ccccaacgca tcatccgcga cctacgcgac caatacctcc gcactctgcc
    16801 cttcgaatcg cttggcttcg aggaaatcaa gcgtaactgc acggactggc ccgaagaatt
    16861 gaccaacttc tctgtctgcg tgacgtacca caacttcgag taccaccccg agagcgaagt
    16921 tgacaaccaa aaggttgaga tgggagtttt ggcaaagtat gttgagttga gtgaaaacga
    16981 gccgctgtac gatcttgcta ttgcgggaga ggttgaggcg gatggagtta acctgaaggt
    17041 cactgttgtt gcaaaggcaa ggctttacaa tgaggcgagg attagacatg tgcttgagga
    17101 agtttgcaaa actttcaatg gtttgaacga ggctttgtaa gtttaaacag tagatgccga
    17161 ccgggatcca cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat
    17221 cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat
    17281 acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac
    17341 aagaataaaa cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcattaa
    17401 tgcattggac ctcgcaaccc tagtacgccc ttcaggctcc ggcgaagcag aagaatagct
    17461 tagcagagtc tattttcatt ttcgggagac gagatcaagc agatcaacgg tcgtcaagag
    17521 acctacgaga ctgaggaatc cgctcttggc tccacgcggg cgcgcctcgg tcgctcactg
    17581 ttcctttacg gatatgtgta ctccggcaag ccgcggggtc gcggtcgttt gtacggcagc
    17641 ttctacttgc ttgcacaggg agctctctgg ggcttgacgt cttttggagt tgcgagggag
    17701 ttgatttcct acttctaagt ttggactgaa tccgtggtgt gattgaggtg attggcgatg
    17761 tttggctata ccagctatat gtaataatct ctactgtata ctactattca acgcatttta
    17821 ctatgcgtgc tgctagggtc ggcaatgaca atggcaatct gactgacgtg gtctatttct
    17881 ccatgtgcag cagggaatac gagctccaat ggacctcggg agtggcacag tcaatggcaa
    17941 ggaaactccg cctttgcagg tgtggctgaa ccccacgggt cggaggcgga gcaatccacc
    18001 cccgatgtgg ctggtgcgtg gaggggctcg cgatgatttt actgagcttg cttttcttgt
    18061 cgacattgaa cattgtcctt ggtcttcctt cagatttaag ggtcagtcac tgctacattt
    18121 ctcagtagta tccgcgcacg tctctggatt tacgaatcag ggtccaccag tcgaaacttc
    18181 gaactactct cattatacaa tcctctttcc attcccgcat taacccctcc atcaacacca
    18241 tgtcctccaa gtcgcaattg acctacactg cccgtgccag caagcatccc aatgctctgg
    18301 cgaagaggct gttcgagatt gccgaggcca agaagaccaa tgtgactgtc tcggctgacg
    18361 ttaccaccac taaggagcta ctagatcttg ctgaccgtag gccgacccgc tactctgcct
    18421 gattatgctg catgcaaact tattaacggt gataccggac tgcaggtctc ggtccctaca
    18481 ttgccgtgat caaaacccac atcgatatcc tctctgattt cagcaacgag accattgagg
    18541 gacttaaggc tctcgcgcag aagcacaact ttctcatctt cgaggaccgc aagttcattg
    18601 acatcggcaa cacggtccag aagcaatacc acggcggtac cctccgtatc tcggaatggg
    18661 cccacatcat caactgcagc attctccctg gtgagggtat cgtcgaggct ctcgctcaga
    18721 cggcgtctgc accggacttc gcctacggcc ccgaacgcgg tctgttgatc ttggcagaga
    18781 tgacctctaa gggctccttg gctaccggcc agtacactac ttcctcggtc gattatgccc
    18841 ggaaatacaa gaacttcgtt atgggattcg tgtcgacgcg cgcgttgggt gaggtgcagt
    18901 cggaagtcag ctctccttcg gatgaggagg actttgtggt cttcacgact ggtgtgaaca
    18961 tttcttccaa gggagataag cttggtcagc agtaccagac gcccggatcg gctatcggcc
    19021 ggggtgctga cttcattatc gcgggtcgcg gtatctacgc cgcgccggat cgatccggtg
    19081 caggctgcgc aacagtatca gaaggagggg tgggaagcct acctggcccg tgtcggcgga
    19141 aactaatact ataaaaggag gatcgaagtt ctgatggtta tgaatgatat agaaatgcaa
    19201 cttgccgcaa cggatacgga agcggaaacg gaccaatgtc gagcacgggt agtcagactg
    19261 cggcatcgga tgtccaaacg gtattgatcc tgcaggctac tatggtgtgg cacaaggatc
    19321 aatgcggtac gacgatttga tgcagataag caggctgcga agtagtaact cttgcgtaga
    19381 gaaaatggcg acgggtgggc tgataagggc ggtgataagc ttaattgtca tcgcagataa
    19441 gcactgctgt cttgcatcca agtcagcgtc agcagaaata cgggacttcc gaaagtatat
    19501 ggcaaaatta aagaacttga ctctccagca atgttttgcc ctgaccgtcg ctaaaacgtt
    19561 actaccccta tacccgtctg tttgtcccag cccgaggcat taggtctgac tgacagcacg
    19621 gcgccatgcg ggcttgggac gccatgtccg tcgcgtgata agggttgatc catgcagcta
    19681 ctatccttcc atcgttccat tcccatcctt gtcctatctc catccttgaa actttactag
    19741 tttagttgga tgctcgagct tgctctcggc tactccgtcg gcgcgccttg cggccgccac
    19801 cgcggtggag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    19861 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    19921 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    19981 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    20041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    20101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    20161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    20221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    20281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    20341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    20401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    20461 tttag
//
