LOCUS       sequence-201152-       20465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of ekivR, esyn1, luc using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 20465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS38.3
FEATURES             Location/Qualifiers
     source          1..20465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          2799..2914
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(2932..3549,3550..3555,3556..3591,3592..3594,3595..3630,
                     3631..3633,3634..3669,3670..3678)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(4026..4044,4045..4067,4068..4086,4087..4109,4110..4128,
                     4129..4151,4152..4170,4171..4193,4194..4212,4213..4235,
                     4236..4254,4255..4277,4278..4296)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          4366..4481
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             5881..5937
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             15343..15399
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(19821..19840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(19822..19840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(19850..19867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(19850..19866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19859..19881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      20008..20463
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(20095..20114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     20305..20326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcggagaa tatggagctt catcgaatca ccggcagtaa
     2281 gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc gaaatagcat gccattaacc
     2341 taggtacaga agtccaattg cttccgatct ggtaaaagat tcacgagata gtaccttctc
     2401 cgaagtaggt agagcgagta cccggcgcgt aagctcccta attggcccat ccggcatctg
     2461 tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg
     2521 ctcaggagct ggccagcggc gcagaccggg aacacaagct ggcagtcgac ccatccggtg
     2581 ctctgcactc gacctgctga ggtccctcag tccctggtag gcagctttgc cccgtctgtc
     2641 cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca gtccaacatt tgttgccata
     2701 ttttcctgct ctccccacca gctgctcttt tcttttctct ttcttttccc atcttcagta
     2761 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
     2821 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
     2881 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccgaattcac catgtctaga
     2941 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
     3001 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     3061 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     3121 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     3181 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     3241 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     3301 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     3361 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     3421 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     3481 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     3541 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     3601 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     3661 gacatgctcc ccgggtaact aagtaaggat ccactagtac agcagaagaa tctctctccg
     3721 ctgttgcttc agtgtctgcc atgcattaac ttcatcctac tgtcctaccc gcagtaccca
     3781 ttcacatttg cggcagatac caggttgttt tctattccct cggttcttca gttcttcaga
     3841 tattacatta ctcgagaaat tggcggcgat gcaggagttt ggttggtttt aattgtttca
     3901 gtctccttga cctgatttgt atgacatgca atgtttcgac ggaagactat ctcggtgaat
     3961 atacgtcatg aatcatgcgg ccgcgcgtat cacgaggccc tttcgtcttc actcgagttt
     4021 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     4081 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4141 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4201 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4261 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccccatc
     4321 ttcagtatat tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta
     4381 catccatact ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc
     4441 acttcatcgc agcttgacta acagctaccc cgcttgagca gacatcaccg tttaaacacc
     4501 atggaaaagc ctgtggcctc acaagtccac cccagctggc taacagctct tctacctgaa
     4561 tccagaatac cccccaagct gtttgcctgg tcctcagcca acatccgccc agcaagctcc
     4621 tccaagctcg gaggctctgc caccaacatc caccatgcag agtgttcaca tccaacccca
     4681 aactccacag caagctctga gcagcgcatc ttcatcgttg gtcccggcaa cataggtagg
     4741 ctctacgcaa gccacatggt ccgccgccca aatcccctcc ccatcactct cgtcgtcccc
     4801 agaaaggaac tcctatcgca atgggcaggc tgtgaaggcg tgggccttat agacccgggt
     4861 cgcggcaagg tcctcaaaag caagcgcttc accgtcgagt ggtggacgga gactagacca
     4921 cagtatggtc ctgtgagaga ggtggcagac ggaaagaagc tgcgcaacgt gttcatatcc
     4981 acaaaggcat ccgctgggct ggccgaggca gatcgtcttc gtcggtattt gggcaagtgt
     5041 agttctgtcg tctttgcgca gaacggagta tgcaagctgt ggcctcctca tgggccgtta
     5101 tacgtcgccg gtcgataccc tcctggtgat gctcctacct tctcatcctg cgtcgtgaat
     5161 catggcgttg catcagcggg gccttttctg agcattcacg cagctccggc agatgcatcc
     5221 atcggacctg tcctctggcc ttcccatccg atgccgcaca agaaacccct gaacgacttc
     5281 ttcacaagat acattgctac tacacccttc ctaaacacaa agcaagtttc ctctggtgag
     5341 ctctggctta ttcagctgga gaaacttgtt accaatgccg ccatcaaccc cctcacagct
     5401 ttgctacgct gcaaaacggg acagctgttc acaacccatg accccacaga ccctcttacg
     5461 cgcgtgctgg acaggttgct atggcaggcc agcgccgtga ttcagggtct tatcaaccac
     5521 gacgcgagtg tcgacatcgt cgcttcctac gccgagcacc tccaccagac cgaaccaagc
     5581 agcgactcaa gccttgtcgc agtacggacc aagttaacag agcggttctc ccagcctacc
     5641 ttgagagcga agctattcgc ctttggtcgc aagatatacg agcatcgcag ctcgatgctt
     5701 caggatgttg aggccggcag gaaaacggag ataagagatt tcaacgggtg ggttgtggat
     5761 atggcgagtt ttctggggac ggggctgaat gtgagcgtgc atcagagttt gattgtgctg
     5821 attgaacgcg gtgaggtttt gaacaaggat gagttggcta ggaggacatt aggctccggc
     5881 gccaccaact tctccctcct gaagcaggcc ggtgacgtcg aggagaaccc cggccccatg
     5941 tcactccaca ccccaagtga cggtcaacaa gacccagcgt tagcctccaa aaccctctgt
     6001 gagcaaatct cgcgagccct cggcctcggc caggacaaga ttgagaatat cttcccaggg
     6061 acacccttcc agcgggacgt catagactgc gccgcagacg acaagcagcg cgccgtgggc
     6121 cacgctgtct ttgaaattcc caaagatata gacgcggccc gcctggcggc tgcgtggaag
     6181 gagaccgtgc ttcacacccc tgctctgcgg acctgcacgt ttacgtccaa atctggggat
     6241 gtgcttcagg tggtcctgag agatagcttt gtcttttcgt ggatgtctgg gccgtctgtg
     6301 gatcttaagg aggcggttgt gcaagacgag gctgctgctg ctttggctgg cccgcgctgt
     6361 aaccgcttcg tccttcttga ggaccctgat acaaaggaac gtcagctcat ctggacgttc
     6421 agccatgctc tcgttgatag taccttccag gaacgtatcc ttcgacgagt cctcaaagct
     6481 tacaaggatg ccaatgacga acatcctcgc cagttcgaga caccagattc ttcccaggcc
     6541 acgcccgagg aggatttaca gcccaatcca tccaagatgc tcaagatccc tcaagccgca
     6601 gatatggacc gtgctgttga gttttggaag gatcatctaa gtggcctgaa tgcttctgcc
     6661 ttcccgcatt tgtcctctca tctgtctatg ccacatccag atgccaaggc agagcataga
     6721 atctcctact cgtcttcagc acagcaaaaa atgtccagtg ctactatttg ccgaactgcc
     6781 ctggcaattc tgttatctcg ttatacgcac tctcctgaag cactctttgg cattgtaact
     6841 gagcaaactc ctctactgga ggagcaactc atgcttgatg gcccaacacg tacagtcgta
     6901 ccaatccgcg tatcctgtgc ttctgaacag tcagtgtcag atatcatgag cacgattgac
     6961 tcctacgatc agaccatgcg gcagtttgcc cacgctggtc ttcgcaacat cgccagtgct
     7021 ggagacgatg aatctgccgc ctgcgggttc cagaccgttc tcttggtctc agatggagac
     7081 gcccagccag catctacttg ggaaattctc aagaaaactg aagagcctga gggtttcatc
     7141 ccctgtacga accgtgctct gcttctaagt tgccagatga ccagctctgg agcacaccta
     7201 accgccagat acgaccagag tatcatcgac gctgagcaaa tggcccgact cctgcgacaa
     7261 ctaggccatc tgatccaaaa tcttcaaacc tccacagatc tacccgtgga aaaggtggac
     7321 atgatgacgc aagaagactg gttagagatt gagaggtgga actcagactc catcgacgca
     7381 caagacacgc ttatccacag cgagatgttg aagtggactt ctcagtcgcc taacaaagct
     7441 gccgtcgccg cgtgggatgg agagtggacg tatgctgagc tggacaatgt atcttcgcgc
     7501 ctggctcagc atatcaactc tattgacctg ggcaaggagc acgcgatagt acccatctac
     7561 tttgagaaat cgaaatgggt cgttgcttcg atgctggctg tgctcaaggc tggccatgcg
     7621 tttactctca ttgaccccag tgatccacca gctcgaacag cacaggtcgt gcagcaaacc
     7681 tctgcgacag ttgctcttac ttccaagctc catcgcgaga ctgttcaatc aactgtcggg
     7741 cgctgcattg ttgtcgacga agagtttgtc aaatccctcc cccagtccag cgaactctca
     7801 gcgtctgtaa aggcacatga cctggcatat gtcatcttca cctcgggcag cacaggcatt
     7861 ccaaagggaa tcatgattga gcaccgctca ttttcatcct gcgccatcaa attcggtccc
     7921 gcgcttggaa taacctccga cacacgcgct cttcagtttg gatctcatgc ctttggcgcg
     7981 tgtatcctcg agatcatgac gacgctcatc cacggaggct gtgtctgtat cccctctgac
     8041 gatgaccgca tgaacaacgt ccttgagttc atcaaccgga ctaacgtcaa ctgggtcatg
     8101 gcaacgcctt cgtacatggg gacattccag ccagaggttg ttcctggttt gaagaccttg
     8161 gtattggtag gtgagcagat gtctgcttct gtgaatgagg tctgggctcc tcgtgttcaa
     8221 ctcctgaacg gttacggaca gagcgagagt tcttctatct gctgcgtagc caagatcagc
     8281 cctggttcat ctgagccaaa caatattggc cacgcagtag gcgcacattc ttggatcgtt
     8341 gaccctgagg atcccaatcg cttggctccc atcggtgccg ttggcgagct ggtcatcgag
     8401 agtgcaggta tcgcacgcga ttacatcgtt gcgccaactc aggataagtc tcctttcatt
     8461 aagactgctc ctacgtggta tccggccaag cagctcccgg acggcttcaa gatctacaga
     8521 acaggcgatc ttgcatgcta cgcatctgat ggcagtatag tgtgccttgg ccgtatggac
     8581 tcccaggtca agatcagagg acagcgtgtt gagctgggtg cagtggagac tcatcttcga
     8641 cagcagatgc ctgatgatat gaccatcgtt gttgaagctg tcaagttctc tgattcctcc
     8701 agtactacag ttttgacagc cttcttgatt ggtgcagggg agaagaacag tcatatcttg
     8761 gaccagcgtg ctacaaggga gatcaatgcc aagatggagc aagttcttcc tcgccattcc
     8821 atccccgcgt tctacatctc tatgaacaac ctcccccaaa cagccactgg taaggtcgat
     8881 cgacgaaaac ttcgtatcat gggtagcaag atactgagcc aaaagacgca ttccacccca
     8941 tctcagcaga gtcaggccgc catctcatct ggaaccgaca cagagaccaa gctggagagc
     9001 atctggatca caagcttgga cctcgagccc ggctctgcaa acatgtcagc tacattcttc
     9061 gagatgggcg gaaactccat catcgcaatc aagatggtca acatggcgcg ctccaatggc
     9121 atagagctta aggtctcgga catttaccag aaccccacgc tcgctggtct caaggctatt
     9181 gtcattggta cttcgctgcc atacagcctt attcccaagg ttacccgcca aggccctgtt
     9241 agcgagcagt cttatgcgca aaacagaatg tggttcctgg atcagttgtc tgagggtgct
     9301 tcatggtatc tgattccttt cgctgtgcgc atgcgaggtc cggtggatgt tgatgcgttg
     9361 acgcgtgctt tgctcgctct tgaacagcgt catgagacac tacgaactac atttgagaac
     9421 caagatggtg ttggagtcca gatcatccat gatagactct ccaaagagct acaagtcatc
     9481 gatgccttgg atggtgacga gggtggtctc aagacactct acaaagtaga gaccaccaca
     9541 ttcgacatca catccgaagc aggctggagc tcaaccctca tccgcctcgg caaagacgat
     9601 cacattctgt ctatcgtcat gcaccacatc atctccgacg gctggtccat cgacgttctc
     9661 cgccgcgaac tcatccaact ctacgccgcc gctctgcagg gcaaggatcc ttcctccgca
     9721 ctaacccccc tacccatcca gtacagcgac ttcgccgtgt ggcagaagca ggaggcccaa
     9781 gcagctgagc acgagaggca gctccagtac tggaagaagc aactcgcaga tagttcacct
     9841 gccaagatcc ctaccgactt cccccgtcca gatctcctgt ccggtgacgc aggcgttgtg
     9901 cccgttgcca tcgacggcga gctgtatcag aaactaaggg gcttctgcaa caagcacaac
     9961 agcactgcct tctccatcct gctcgctgct ttccgcgcgg cgcattaccg tctcacagcc
    10021 gttgacgacg ccgtgatcgg catccccatt gcaaaccgta accgctggga gctggagaac
    10081 atgattggtt tctttgtcaa cacgcagtgt atgcgcatcg ccgttgacga gacggataca
    10141 tttgagagtc tggtgcgcca ggtcagatct accactacag ctgcgtttgc gcacgaggat
    10201 gtccccttcg agcgtgtcgt ttcagcgctt cagcctggcc atagagatct ctcgcgaaca
    10261 ccgctggcac agataatgtt tgctgttcac tcgcagaagg accttggacg tttcgagctg
    10321 gagggtatcc agtctgagcc tatcgccagc aaggcctaca ccagattcga tgtcgagttc
    10381 catctgttcc aacaggcaga cggactgaag ggcagttgca actttgccac agatctcttc
    10441 aagcccgaaa ctatccagaa tgttgtcagc gtgtttttcc agattctacg ccatggcctt
    10501 gaccagcctg agacgtgtat ctcggttctt ccactgactg atggagtcga ggagcttcgc
    10561 aggttggatc tgctggaaat caagaggact aactaccctc gcgattcgag cgtggtagat
    10621 gtcttccgcg aacaagctgc cgccaaccca gaggttatcg ctgttaccga ctcatcttct
    10681 cgtctgactt atgcagagct ggacaataag tctgagctgc tctcacgatg gcttcgacga
    10741 cgtaacttga cgccagagac gctggtcagt gttcttgctc cccggtcttg cgagactatc
    10801 gttgcctatg ttggtatcct caaggcgaac ctggcgtatc ttcctcttga cgtgaggtcc
    10861 ccggtgactc gtatgaagga catcttgtcg agcgtgtctg gaaacaccat agttcttatg
    10921 ggctctgggg tagaggatcc tggctttgac ttaccgcaac tagagctcgt acgcatcacc
    10981 gacactttcg atgagaccat cgaggacgtg caagactctc cccaaccgtc tgccacaagc
    11041 ctcgcctacg tcgtcttcac atccggttca actggtaaac ccaagggcgt catgatcgag
    11101 caccgggcca ttgtgcgtct cgtcaagagt gacaactttc ctggcttccc ctcccccgct
    11161 cgcatgtcaa atgtcttcaa ccctgccttc gacggagcca tctgggagat caactggatg
    11221 ctcctgaacg gcggaacagt cgtctgcatc gactacctga ccaccctgga cggcaaagag
    11281 ctcgctgctg tgttcgccaa agagcgcgtc aacgccgcct tcttcgcacc tgcgatgctc
    11341 aagctttacc tcgttgatgc gcgcgaggct ttgaagaatc ttgacttcct tattgttgga
    11401 ggtgagaggt tcgataccaa ggaagccgtg gaggccatgc cgcttgtgag aggcaagatt
    11461 gccaatatct atggtccgac tgaggctgga ataatcagca cgtgctataa catccccaag
    11521 gatgaggctt acaccaatgg tgttcccatt ggtggaagta tctacaactc tggtgcctac
    11581 gtcatggatc ctaaccagca acttgtcggc cttggcgtca tgggagagct tgttgttacc
    11641 ggagacggtg ttggtcgagg ctacactaac cccgagctca acaagaaccg cttcatcgac
    11701 atcaccatcg agggcaagac tttcaaggct taccgtactg gtgaccggat gcgtgcacga
    11761 gtaggcgacg gtctccttga gttcttcggc cgcatggaca accagttcaa gatccgcggc
    11821 aaccgtatcg aagcagggga agttgagtct gccatgctca gcctcaagaa tgtccttaac
    11881 gccgccattg tcgtccgcgg gggcggagaa gatgaagggc cactcgagat ggtcggattc
    11941 atcgtcgcgg acgacaagaa tgataccacg gaggaggaag agacaggcaa ccaagttgag
    12001 ggctggcagg accatttcga gagtggtatg tactcggata tcagcaccgc cgtggaccaa
    12061 tctgccattg gaaacgactt taagggctgg acttctatgt acgatggtaa ggatatcgac
    12121 aagggcgaga tgcaggagtg gttggacgac gctattcaca ccctgcataa cggccagatc
    12181 cctcgcgatg tcctcgagat cggtaccggt agtggtatga tcctgttcaa cctcaacccg
    12241 ggcctcaaca gctacgttgg tcttgatcca tccaagtcag cagtcgagtt cgtcaacaga
    12301 gccgtcgagt cctctcccaa gttcgcagga aaagccaagg tccacgtcgg catggccaca
    12361 gacgtcaaca aactcggcga agtacacccc gatctcgtgg tcttcaactc cgttgttcaa
    12421 tacttcccca cacccgagta tctcgccgag gtcatcgatg gcctcattgc catccccagc
    12481 gtcaagcgca tcttccttgg cgatatcaga tcatatgcta ccaacggaca cttcctcgcc
    12541 gcacgcgcca tccacacgct cggcaccaat aacaacgcca ccaaggatag ggtgcgccag
    12601 aagatccagg agctggagga tcgagaggaa gagtttctcg ttgagcctgc cttcttcacc
    12661 actctgaagg agcgacgtcc agatgttgtc aagcatgttg agatcatccc caagaacatg
    12721 aaggccacca acgaactcag cgcctatcgc tacacggctg ttgtgcatct gcgggatgaa
    12781 acggacgaac ctgtgtatca tattgagaag gatagctggg ttgactttga ggcgaagcag
    12841 atggacaaga cggctcttct tgaccacctg cgcctctcca aggatgctat gagtgtggcg
    12901 gttagcaaca tcacctacgc ccacactgcc tttgaacgtc gtatcgttga gtctctcgat
    12961 gaggatagca aggatgacac caagggtaca ctcgatggtg cagcctggct ctcagcagtt
    13021 cgctccgaag ccgaaaaccg cgcctcactc accgtccccg acatcctgga gatcgccaaa
    13081 gaggctggtt tccgagttga agtcagcgct gctcgccagt ggtcccaaag tggtgcttta
    13141 gacgcagtct tccaccactt tccaccctcc agcactgacc gcactctaat ccagttcccc
    13201 acggacaacg agcttcgatc atcactcacc ctcgccaacc gccctctcca gaagctgcag
    13261 cgccgtcgtg ccgctctgca agtccgcgag aagctccaga cgctggtccc gtcttacatg
    13321 gttcctccga atatcgtggt gctggacacg atgcctctca atactaacgg caagatcgac
    13381 agaaaggagc ttacgcgtag agcacgaaca ctgccgaagc agcagactgc ggcgcctgtg
    13441 ccggacttcc ctatctctga tatcgagatc acgctgtgcg aggaggcaac tgaggtcttt
    13501 ggaatgaagg ttgaaatcag cgatcacttc ttccagctcg gcggtcactc tctcctcgct
    13561 acgaaactca tttctcgcat ccagcaccgt ctccatgtgc gggttactgt gaaggacgta
    13621 ttcgacagcc ctgtctttgc cgatctggca gtcatcatcc gtcaaggact tgctatgcag
    13681 aaccctgttg ctgaaggaca ggacaagcaa ggctggtcct cgagagttgc ccctcgtaca
    13741 gaagtcgaga agatgctgtg cgaggagttc gcagctggtc ttggtgtccc ggttggtatc
    13801 actgacaact tcttcgatct cggtggtcac tcgctcatgg ctactaagct agctgtgcga
    13861 attggccgtc gccttgatac cgccatcaca gtcaaggaca tcttcgacta ccctgtgctt
    13921 ttccaattgg cgaagaagtt ggagtcttcg cattccaaga gctacgagga gtctggcgac
    13981 gatatccaga tggccgatta cactgcattc cagctcctcg atctggaaga cccccaagac
    14041 tttgttcagt cccagattcg gcctcaactg gactcctgct acggcaccat ccaggatgtc
    14101 tacccgtcta cgcagatgca aaaggccttc ctcttcgatc ccacgaccgg cgagccccga
    14161 ggtcttgtgc ctttctatat cgacttcccc agcaatgcag atgccgagac cctcaccaag
    14221 gctatcggag ctctagttga caagctcgat atgttcagaa ctgtcttcct ggaggccgca
    14281 ggcgatctgt accaagttgt cgttgagcac ctcaacttgc ctattgagac catcgagact
    14341 gagaagaacg tcaacactgc aaccggtgac tacctggatg ttcatggaaa ggaccctgtc
    14401 cgtctaggcc acccgtgcat ccaattcgcc atcctgaaga ctgcctcctc tgtacgtgtt
    14461 ctcctgcgaa tgtcccacgc tctgtatgat ggtttgagtt ttgagtacat cgtccgtggt
    14521 ctccacgttc tctacagcgg tagaaacctc cccccaccaa cacagtttgc gcgatacatg
    14581 cagtatgctg cacacagtcg tgaagaaggt tatcccttct ggcgcgaggt tctgcagaac
    14641 gcgcccatga cagttctaca cgacaccaat aacggtatgt ctgagcaaga gatgccagcc
    14701 tccaaggcgg tacacctgtc agaggtcgtc aacgttccag cgcaagctat tcgaaacagt
    14761 accaacaccc aagcgaccgt cttcaacacc gcctgtgccc ttgtcctagc caaggaatcc
    14821 ggctcacagg atgtcgtctt cggccgtatt gtctctggtc gacaaggtct accagtcgtc
    14881 tggcaggaca tcatcggccc ctgcacaaac gccgtgcccg tccacgcacg cgtcgacgat
    14941 ggaaaccccc aacgcatcat ccgcgaccta cgcgaccaat acctccgcac tctgcccttc
    15001 gaatcgcttg gcttcgagga aatcaagcgt aactgcacgg actggcccga agaattgacc
    15061 aacttctctg tctgcgtgac gtaccacaac ttcgagtacc accccgagag cgaagttgac
    15121 aaccaaaagg ttgagatggg agttttggca aagtatgttg agttgagtga aaacgagccg
    15181 ctgtacgatc ttgctattgc gggagaggtt gaggcggatg gagttaacct gaaggtcact
    15241 gttgttgcaa aggcaaggct ttacaatgag gcgaggatta gacatgtgct tgaggaagtt
    15301 tgcaaaactt tcaatggttt gaacgaggct ttgggctccg gcgccaccaa cttctccctc
    15361 ctgaagcagg ccggtgacgt cgaggagaac cccggcccca tggaggacgc caagaacatc
    15421 aagaagggcc ccgccccctt ctaccccctc gaggacggca ccgccggcga gcagctccac
    15481 aaggccatga agcgctacgc cctcgtcccc ggcaccatcg ccttcgtagg tttcctccag
    15541 ctctcgcctc cagcacccga ggcacacctc gggcatcttc acaacaacag acactgacat
    15601 ctcattctca cagaccgacg cccacatcga ggtcaacatc acctacgccg agtacttcga
    15661 gatgtccgtc cgcctcgccg aggccatgaa gcgctacggc ctcaacacca accaccgcat
    15721 cgtcgtctgc tccgagaact ccctccagtt cttcatgccc gtcctcggcg ccctcttcat
    15781 cggcgtcgcc gtcgcccccg ccaacgacat ctacaacgag cgcgagctcc tcaactccat
    15841 gaacatctcc cagcccaccg tcgtcttcgt ctccaagaag ggcctccaga agatcctcaa
    15901 cgtccagaag aagctcccca tcatccagaa gatcatcatc atggactcca agaccgacta
    15961 ccagggcttc cagtccatgt acaccttcgt cacctcccac ctcccccccg gcttcagcga
    16021 gtacgacttc gtccccgagt ccttcgaccg cgacaagacc atcgccctca tcatgaactc
    16081 ctccggctcc accggcctcc ccaagggcgt cgccctcccc caccgcaccg cctgcgtccg
    16141 cttctcccac gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgccatcct
    16201 ctccgtcgtc cccttccacc acggcttcgg catgttcacc accctcggct acctcatctg
    16261 cggcttccgc gtcgtcctca tgtaccgctt cgaggaggag ctcttcctcc gctccctcca
    16321 ggactacaag atccagtccg ccctcctcgt ccccaccctc ttctccttct tcgccaagtc
    16381 caccctcatc gacaagtacg acctctccaa cctccacgag atcgcctccg gcggcgcccc
    16441 cctctccaag gaggtcggcg aggccgtcgc caagcgcttc cacctccccg gcatccgcca
    16501 gggctacggc ctcaccgaga ccacctccgc catcctcatc acccccgagg gcgacgacaa
    16561 gcccggcgcc gtcggcaagg tcgtcccctt cttcgaggcc aaggtcgtcg acctcgacac
    16621 cggcaagacc ctcggcgtca accagcgcgg cgagctctgc gtccgcggcc ccatgatcat
    16681 gtccggctac gtcaacaacc ccgaggccac caacgccctc atcgacaagg acggctggct
    16741 ccactccggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tcgaccgcct
    16801 caagtccctc atcaagtaca agggctacca ggtcgccccc gccgagctcg agtccatcct
    16861 cctccagcac cccaacatct tcgacgccgg cgtcgccggc ctccccgacg acgacgccgg
    16921 cgagctcccc gccgccgtcg tcgtcctcga gcacggcaag accatgaccg agaaggagat
    16981 cgtcgactac gtcgcctccc aggtcaccac cgccaagaag ctccgcggcg gcgtcgtctt
    17041 cgtcgacgag gtccccaagg gcctcaccgg caagctcgac gcccgcaaga tccgcgagat
    17101 cctcatcaag gccaagaagg gcggcaagtc caagctctaa gtttaaacag tagatgccga
    17161 ccgggatcca cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat
    17221 cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat
    17281 acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac
    17341 aagaataaaa cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcattaa
    17401 tgcattggac ctcgcaaccc tagtacgccc ttcaggctcc ggcgaagcag aagaatagct
    17461 tagcagagtc tattttcatt ttcgggagac gagatcaagc agatcaacgg tcgtcaagag
    17521 acctacgaga ctgaggaatc cgctcttggc tccacgcggg cgcgcctcgg tcgctcactg
    17581 ttcctttacg gatatgtgta ctccggcaag ccgcggggtc gcggtcgttt gtacggcagc
    17641 ttctacttgc ttgcacaggg agctctctgg ggcttgacgt cttttggagt tgcgagggag
    17701 ttgatttcct acttctaagt ttggactgaa tccgtggtgt gattgaggtg attggcgatg
    17761 tttggctata ccagctatat gtaataatct ctactgtata ctactattca acgcatttta
    17821 ctatgcgtgc tgctagggtc ggcaatgaca atggcaatct gactgacgtg gtctatttct
    17881 ccatgtgcag cagggaatac gagctccaat ggacctcggg agtggcacag tcaatggcaa
    17941 ggaaactccg cctttgcagg tgtggctgaa ccccacgggt cggaggcgga gcaatccacc
    18001 cccgatgtgg ctggtgcgtg gaggggctcg cgatgatttt actgagcttg cttttcttgt
    18061 cgacattgaa cattgtcctt ggtcttcctt cagatttaag ggtcagtcac tgctacattt
    18121 ctcagtagta tccgcgcacg tctctggatt tacgaatcag ggtccaccag tcgaaacttc
    18181 gaactactct cattatacaa tcctctttcc attcccgcat taacccctcc atcaacacca
    18241 tgtcctccaa gtcgcaattg acctacactg cccgtgccag caagcatccc aatgctctgg
    18301 cgaagaggct gttcgagatt gccgaggcca agaagaccaa tgtgactgtc tcggctgacg
    18361 ttaccaccac taaggagcta ctagatcttg ctgaccgtag gccgacccgc tactctgcct
    18421 gattatgctg catgcaaact tattaacggt gataccggac tgcaggtctc ggtccctaca
    18481 ttgccgtgat caaaacccac atcgatatcc tctctgattt cagcaacgag accattgagg
    18541 gacttaaggc tctcgcgcag aagcacaact ttctcatctt cgaggaccgc aagttcattg
    18601 acatcggcaa cacggtccag aagcaatacc acggcggtac cctccgtatc tcggaatggg
    18661 cccacatcat caactgcagc attctccctg gtgagggtat cgtcgaggct ctcgctcaga
    18721 cggcgtctgc accggacttc gcctacggcc ccgaacgcgg tctgttgatc ttggcagaga
    18781 tgacctctaa gggctccttg gctaccggcc agtacactac ttcctcggtc gattatgccc
    18841 ggaaatacaa gaacttcgtt atgggattcg tgtcgacgcg cgcgttgggt gaggtgcagt
    18901 cggaagtcag ctctccttcg gatgaggagg actttgtggt cttcacgact ggtgtgaaca
    18961 tttcttccaa gggagataag cttggtcagc agtaccagac gcccggatcg gctatcggcc
    19021 ggggtgctga cttcattatc gcgggtcgcg gtatctacgc cgcgccggat cgatccggtg
    19081 caggctgcgc aacagtatca gaaggagggg tgggaagcct acctggcccg tgtcggcgga
    19141 aactaatact ataaaaggag gatcgaagtt ctgatggtta tgaatgatat agaaatgcaa
    19201 cttgccgcaa cggatacgga agcggaaacg gaccaatgtc gagcacgggt agtcagactg
    19261 cggcatcgga tgtccaaacg gtattgatcc tgcaggctac tatggtgtgg cacaaggatc
    19321 aatgcggtac gacgatttga tgcagataag caggctgcga agtagtaact cttgcgtaga
    19381 gaaaatggcg acgggtgggc tgataagggc ggtgataagc ttaattgtca tcgcagataa
    19441 gcactgctgt cttgcatcca agtcagcgtc agcagaaata cgggacttcc gaaagtatat
    19501 ggcaaaatta aagaacttga ctctccagca atgttttgcc ctgaccgtcg ctaaaacgtt
    19561 actaccccta tacccgtctg tttgtcccag cccgaggcat taggtctgac tgacagcacg
    19621 gcgccatgcg ggcttgggac gccatgtccg tcgcgtgata agggttgatc catgcagcta
    19681 ctatccttcc atcgttccat tcccatcctt gtcctatctc catccttgaa actttactag
    19741 tttagttgga tgctcgagct tgctctcggc tactccgtcg gcgcgccttg cggccgccac
    19801 cgcggtggag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    19861 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    19921 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    19981 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    20041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    20101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    20161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    20221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    20281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    20341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    20401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    20461 tttag
//
