LOCUS       sequence-201153-        2687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LI promoter module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Wu D, Schandry N, Lahaye T
  TITLE     A modular toolbox for Golden-Gate-based plasmid assembly streamlines
            generation of Ralstonia solanacearum species complex knockout 
            strains and multi-cassette complementation constructs.
  JOURNAL   Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632.
  PUBMED    29077245
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632."
COMMENT     Sequence Label: LI A-B phrpB
FEATURES             Location/Qualifiers
     source          1..2687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(372..394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    383..399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(407..413,414..431,432..437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    452..473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(590..607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(761..1349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(841..860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1464..1469,1470..1486,1487..1492)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1662..2195
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     2282..2300
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2338..2360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2460..2479
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(2673..2687,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2687,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttcgagctcg gtacctcgcg aatgcatcta gatatcggat
       61 cccgggcccg tcgactgcag aggtacgggt ctcagcggag gtcaagggta cgctcaccta
      121 cagccagttg aaagagcagg tgaagcaagg attggtgggt gacggcacgt cgccaaaagc
      181 gaaaactcca cagtgaggat tgttcagggc aaggcgcagc tttgttgaga ttatttttcc
      241 ggcatttttc tacgatgact ccaggcagtt tgattgatgc ggcctgcatg tcgcgggctg
      301 gttcgactga cgcttctctg cgagacctct gcctgcatgc aagcttggcg taatcatggt
      361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1201 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaggcgc acaccgtgga
     1441 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca
     1501 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1561 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg
     1621 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1681 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca
     1741 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt
     1801 tcggtcgtga gttcggcgac gtagccacct actcccaaca tcagccggac tccgattacc
     1861 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg
     1921 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct
     1981 atatctatga tctcgcagtc tccggagagc accggaggca gggcattgcc accgcgctca
     2041 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag
     2101 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga
     2161 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct
     2221 tcccggccgc ggagttgttc ggtaaattgt cacaacgccg ctcatgacat taacctataa
     2281 aaataggcgt atcacgaggc ccttttgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     2341 ctgacacatg cagctcccgg agaaggtcac agcttgtctg taagcggatg ccgggagcag
     2401 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     2461 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     2521 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     2581 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     2641 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
